5-hmC Antibody (13612) - Azide and BSA Free

Immunohistochemistry-Paraffin: Antibody (GT13612) - Detection of protein at nucleus on mouse testis. Dilution: 1:100

Immunohistochemistry-Paraffin: Antibody (GT13612) - Detection of protein at nucleus on mouse fore brain. Dilution 1:100

Immunohistochemistry-Paraffin: Antibody (GT13612) - Detection of protein at nucleus on mouse prostate. Dilution: 1:100

Dot Blot: Antibody (GT13612) - Analysis of antibody with the synthetic DNA controls. DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine.

• Reactivity: All-NA
• Applications: DB, IHC, IP, MS
• Clone: 13612
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

5-hmC Antibody (13612) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse 5-hmC Antibody (13612) - Azide and BSA Free (NBP2-42219) is a monoclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen used to generate this antibody corresponds to 5-HydroxymethylCytosine / 5-hmC
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Electron Microscopy
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
  • Methylated DNA Immunoprecipitation
• Application Notes: IHC-FR, DOT, EM, MEDIP-Assay dependent

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for 5-hmC Antibody (13612) - Azide and BSA Free

• 5'-hmC
• 5-hydroxymethylcytosine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.