HAF007). A specific band was detected for SOCS-3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for SOCS-3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, U937 human histiocytic lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse SOCS-3 Monoclonal Antibody (Catalog # MAB5696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS-3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB5696) with an ED₅₀ of 0.150-1.50 ng/mL." class="big_lightbox"> MAB5696) with an ED₅₀ of 0.150-1.50 ng/mL." alt="Measured by its binding ability in a functional ELISA. Recombinant Human SOCS‑3 His-tag Protein (Catalog # 11817-S3) binds to Human/Mouse SOCS-3 Antibody (MAB5696) with an ED₅₀ of 0.150-1.50 ng/mL." class="big_thumb" />