| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NBP2-24867 | |||
| BSA Free | NBP2-25186SS | |||
| Low Endotoxin, Azide and BSA Free | NBP2-24867SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9929-SP | |||
| Carrier Free | FAB9929V-100UG | |||
| Carrier Free | FAB9929U-100UG | |||
| Carrier Free | FAB9929T-100UG | |||
| Carrier Free | FAB9929S-100UG | |||
| Carrier Free | FAB9929R-100UG | |||
| Carrier Free | FAB9929N-100UG | |||
| Carrier Free | FAB9929G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54392-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80638 | |||
| BSA Free | NB100-2726SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11411-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54405-100ug | |||
![Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - FFPE human tonsil stained with CD3 antibody.](http://images.novusbio.com/fullsize/CD3-Antibody-SP7-Immunohistochemistry-Paraffin-NB600-1441-img0001.jpg "Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - FFPE human tonsil stained with CD3 antibody.")
![Immunohistochemistry-Frozen: CD3 Antibody (SP7) [NB600-1441] - T-cells and microglia in mouse spinal cord with acute EAE. Mouse was perfused with 4% paraformaldehyde. Spinal cord was post-fixed overnight, followed by cryoprotection with 30% sucrose for 24h. Spinal cord sections were treated with antigen-retrieval buffer (pH 6.0) at 60C for 10 min, then cooled down to room temperature (this step should not be omitted). Anti-CD3 antibody was diluted at 1:200. Incubation: room temperature overnight. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD3-Antibody-SP7-Immunohistochemistry-Frozen-NB600-1441-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - Mouse lung tissue stained against C3aR normal conditions (left) and knockdown conditions (right). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD3-Antibody-SP7-Immunohistochemistry-Paraffin-NB600-1441-img0003.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Alexa Fluor (R) 488 conjugate of NBP2-24867. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0009.jpg "Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Alexa Fluor (R) 488 conjugate of NBP2-24867. A cell surface stain was performed on hPBMCs with CD8 Alpha antibody (RPA-T8) NBP2-25195 (top image) and a matched isotype control NBP2-27287 (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PerCP. A co-stain was also performed using CD3 antibody NBP2-24867AF488.")
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using the Alexa Fluor(R) 700 conjugate of NBP2-24867. Staining of human PBMC. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0008.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using Azide Free version of NBP2-25186. CD3+ hPMBCs using the APC conjugate of this antibody. A cell surface stain was performed on CD3+ hPBMCs with V alpha 24 J alpha 18 TCR antibody (6B11) NBP2-00267PE (top image) and a matched isotype control](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0005.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis of CD3 expression in hPBMC's gated to lymphocyte population at 1 x 10^6 cells/mL. Cells were stained with NBP2-24867PE (orange) conjugate at a 1:500 dilution. Results are shown with mIgG (PE conjugate) isotype control (blue). Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0013.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis of PerCP conjugate of NBP2-25186. A surface stain was performed on Jurkat cells with CD3 (OKT3) antibody NBP2-24867PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0004.jpg)
![Flow Cytometry: CD3 Antibody (OKT3) [NBP2-25186] - Analysis using the Alexa Fluor(R) 488 conjugate of NBP2-24867. Detection of CD3 expression in hPBMc's gated to lymphocyte population at 1 x 10^6 cells/mL. Cells were stained with NBP2-24867AF488 (orange) conjugate at a 1:500 dilution. Results are shown with mIgG2 (AF488 conjugate) isotype control (blue). Image using the Low Endotoxin and Azide Free form of this antibody.](http://images.novusbio.com/fullsize/CD3-Antibody-OKT3-Flow-Cytometry-NBP2-25186-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CD3 Antibody (13) [NBP2-43674] - Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD3 protein stained by CD3 antibody [0013] diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/CD3-Antibody-13-Immunocytochemistry-Immunofluorescence-NBP2-43674-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD3 Antibody (13) [NBP2-43674] - Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD3 protein stained by CD3 antibody [0013] diluted at 1:200. Blue: Hoechst 33342 staining.")
![Flow Cytometry: CD3 Antibody (13) [NBP2-43674] - An intracellular stain was performed on Jurkat cells with CD3 Antibody (13) NBP2-43674F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/CD3-Antibody-13-Flow-Cytometry-NBP2-43674-img0004.jpg)
![Flow Cytometry: CD3 Antibody (13) [NBP2-43674] - Analysis of B6 / spleen. Blue: Isotype control (anti-mouse IgG-FITC) dilution: 1:300. Red: CD3 antibody dilution: 1:50.](http://images.novusbio.com/fullsize/CD3-Antibody-13-Flow-Cytometry-NBP2-43674-img0001.jpg)
![Flow Cytometry: CD3 Antibody (13) [NBP2-43674] - Analysis of Jurkat cell Black: Isotype control dilution: 1:50. Green: CD3 antibody dilution: 1:50.](http://images.novusbio.com/fullsize/CD3-Antibody-13-Flow-Cytometry-NBP2-43674-img0002.jpg)
![Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1308).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Western-Blot-NBP2-53387-img0005.jpg "Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1308).")
![Flow Cytometry: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Flow Cytometric Analysis of Jurkat cells using CD3 epsilon Antibody (C3e/1308) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Flow-Cytometry-NBP2-53387-img0004.jpg)
![Western Blot: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Western Blot of recombinant CD3e and Jurkat Cell Lysate using CD3e Monoclonal Antibody (C3e/1308)](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-C3e-1308-Western-Blot-NBP2-53387-img0002.jpg)
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody (C3e/1308) [NBP2-53387] - Staining of T cells in human FFPE tonsil tissue using AF647-conjugated CD3 epsilon Antibody (Catalog # NBP2-54392AF647). Antigen Retrieval at pH 9. Antibody incubation over night at 4C. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-53387_mouse-monoclonal-cd3-epsilon-antibody-c3e-1308-17720241003230.jpg)
![Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E (red) in FFPE lung carcinoma by IHC-IF. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Immunocytochemistry-Immunofluorescence-NBP2-76424-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E (red) in FFPE lung carcinoma by IHC-IF. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).")
![Western Blot: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E by WB of Jurkat, MOLT-4, K-562, SK-MEL-28 (SK), and HeLa lysate Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Actin.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Western-Blot-NBP2-76424-img0003.jpg)
![Immunohistochemistry: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E in FFPE tonsil by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Immunohistochemistry-NBP2-76424-img0001.jpg)
![Flow Cytometry: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E in Jurkat cells by flow cytometry. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424) (red shaded) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Flow-Cytometry-NBP2-76424-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E in FFPE MOLT-4 cells (left) and Ramos cells (right) by ICC. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Immunocytochemistry-NBP2-76424-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E (red) in FFPE appendix by IHC-IF. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] NBP2-76424. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Immunocytochemistry-Immunofluorescence-NBP2-76424-img0004.jpg)
![Immunoprecipitation: CD3 epsilon Antibody (BL-298-5D12) [NBP2-76424] - Detection of human CD3E by WB of immunoprecipitates from Jurkat lysate. Antibodies: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (NBP2-76424).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-BL-298-5D12-Immunoprecipitation-NBP2-76424-img0007.jpg)
![Western Blot: CD3 epsilon Antibody (UCHT1) [NB100-2726] - Western blot shows cell lysates MOLT-4, Jurket, Raji, and THP-1. Membrane was probed with 2 ug/mL of Mouse Anti-CD3 epsilon Monoclonal Antibody (Catalog # NB100-2726) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated) in the first 2 cell lysates but was absent from the later 2. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-UCHT1-Western-Blot-NB100-2726-img0003.jpg "Western Blot: CD3 epsilon Antibody (UCHT1) [NB100-2726] - Western blot shows cell lysates MOLT-4, Jurket, Raji, and THP-1. Membrane was probed with 2 ug/mL of Mouse Anti-CD3 epsilon Monoclonal Antibody (Catalog # NB100-2726) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD3 epsilon at approximately 21 kDa (as indicated) in the first 2 cell lysates but was absent from the later 2. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. Internally validated Western blot.")
![Flow Cytometry: CD3 epsilon Antibody (UCHT1) [NB100-2726] - Analysis of PE/Cy5 conjugate of NB100-2726. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy5 (green) or Anti-Human CD3 PE-Cy5 (blue). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-UCHT1-Flow-Cytometry-NB100-2726-img0002.jpg)
![Flow Cytometry: CD3 epsilon Antibody (UCHT1) [NB100-2726] - Staining of human peripheral blood lymphocytes with Mouse anti-Human CD3: RPE.](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-UCHT1-Flow-Cytometry-NB100-2726-img0001.jpg)
![Dual RNAscope ISH-IHC: CD3 epsilon Antibody (UCHT1) [NB100-2726] - CD40 mRNA (red) and CD3 epsilon protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human malignant lymph node. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD40 and CD3 epsilon antibody (UCHT1) at 1:300 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-UCHT1-Dual-RNAscope-ISH-IHC-NB100-2726-img0004.jpg)
![Flow Cytometry: CD3 Antibody (SK7) [NBP3-09140] - Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (SK7) purified antibody (concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/CD3-Antibody-SK7-Flow-Cytometry-NBP3-09140-img0002.jpg "Flow Cytometry: CD3 Antibody (SK7) [NBP3-09140] - Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (SK7) purified antibody (concentration in sample 4 ug/ml) GAM APC.")
![Flow Cytometry: CD3 Antibody (SK7) [NBP3-09140] - Surface staining pattern of human peripheral whole blood stained using anti-human CD3 (SK7) purified antibody (concentration in sample 4 ug/ml) GAM APC](http://images.novusbio.com/fullsize/CD3-Antibody-SK7-Flow-Cytometry-NBP3-09140-img0001.jpg)
![Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Jurkat Cell lysate using CD3 epsilon antibody (PC3/188A).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-PC3-188A-Western-Blot-NBP2-53386-img0007.jpg "Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Jurkat Cell lysate using CD3 epsilon antibody (PC3/188A).")
![Immunohistochemistry-Paraffin: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD3 epsilon Monoclonal Antibody (PC3/188A).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-PC3-188A-Immunohistochemistry-Paraffin-NBP2-53386-img0001.jpg)
![Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Thymus lysate using Purified CD3 epsilon Antibody (PC3/188A).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-PC3-188A-Western-Blot-NBP2-53386-img0005.jpg)
![Western Blot: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (PC3/188A).](http://images.novusbio.com/fullsize/CD3-epsilon-Antibody-PC3-188A-Western-Blot-NBP2-53386-img0006.jpg)
![Flow Cytometry: CD3 epsilon Antibody (PC3/188A) [NBP2-53386] - Analysis with canine whole blood cells. From a verified customer review.](http://images.novusbio.com/fullsize/nbp2-53386_mouse-monoclonal-cd3-epsilon-antibody-pc3-188a-177202495940.jpg)
![Immunohistochemistry-Paraffin: CD3 Antibody (N26-R) [NBP1-79054] - CD3 positivity in T-lymphocytes of the normal lymph node, stained with anti-CD3 antibody. FFPE human tissue (4 um section) stained.](http://images.novusbio.com/fullsize/CD3-Antibody-N26-R-Immunohistochemistry-Paraffin-NBP1-79054-img0003.jpg "Immunohistochemistry-Paraffin: CD3 Antibody (N26-R) [NBP1-79054] - CD3 positivity in T-lymphocytes of the normal lymph node, stained with anti-CD3 antibody. FFPE human tissue (4 um section) stained.")