459-MO) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog #
375-TL) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (20 ng/mL) activity elicited by Recombinant Mouse OPG/TNFRSF11B Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse OPG/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459). The ND
50 is typically 0.15-0.9 µg/mL." class="big_lightbox">

459-MO) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog #
375-TL) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (20 ng/mL) activity elicited by Recombinant Mouse OPG/TNFRSF11B Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse OPG/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459). The ND
50 is typically 0.15-0.9 µg/mL." alt="In the presence of the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Mouse OPG/TNFRSF11B Fc Chimera (Catalog #
459-MO) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog #
375-TL) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (20 ng/mL) activity elicited by Recombinant Mouse OPG/TNFRSF11B Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse OPG/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459). The ND
50 is typically 0.15-0.9 µg/mL." class="big_thumb" />
459-MO) was serially diluted and captured by Rat Anti-Mouse Osteoprotegerin/TNFRSF11B Monoclonal Antibody (Catalog #
MAB4591) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Mouse Osteoprotegerin/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">

459-MO) was serially diluted and captured by Rat Anti-Mouse Osteoprotegerin/TNFRSF11B Monoclonal Antibody (Catalog #
MAB4591) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Mouse Osteoprotegerin/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />