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![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Immunoblot analyses of protein expression for Nrf2 and antioxidant enzymes. Representative immunoblots of cytosolic extracts from the hearts of young and old mice under basal conditions and following EES. Protein blots were probed with anti-HO1, NQO1, GCLM, GCLC, Catalase, SOD1, SOD2, GSR, G6PD, GPX1 and GAPDH. Individual lanes indicate a single animal. Densitometry analysis of respective protein signals was performed using Image-J and expressed as relative intensity units calculated as mean values of young and old, *p<0.05. Individual lanes indicate each animal (n?=?6). #p<0.05-between basal and EES. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0045697), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NB100-236-img0009.jpg "Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Immunoblot analyses of protein expression for Nrf2 and antioxidant enzymes. Representative immunoblots of cytosolic extracts from the hearts of young and old mice under basal conditions and following EES. Protein blots were probed with anti-HO1, NQO1, GCLM, GCLC, Catalase, SOD1, SOD2, GSR, G6PD, GPX1 and GAPDH. Individual lanes indicate a single animal. Densitometry analysis of respective protein signals was performed using Image-J and expressed as relative intensity units calculated as mean values of young and old, *p<0.05. Individual lanes indicate each animal (n?=?6). #p<0.05-between basal and EES. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0045697), licensed under a CC-BY licence.")
![<b>Biological Strategies Validation. </b>Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of Human and Mouse G6PD by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G6PD antibody NB100-236 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NB100-236-img0007.jpg)
![Immunoprecipitation: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of mouse G6PD by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-G6PD antibody NB100-236 (lot 2) used for IP at 6 ug per reaction. G6PD was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated G6PD, NB100-236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunoprecipitation-NB100-236-img0008.jpg)
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysates.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-(2H7)-Western-Blot-NBP2-22125-img0003.jpg "Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysates.")
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of paraffin-embedded kidney cancer tissues using Glucose 6 Phosphate Dehydrogenase mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-(2H7)-Immunohistochemistry-Paraffin-NBP2-22125-img0006.jpg)
![Flow Cytometry: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of Jurkat cells using Glucose 6 Phosphate Dehydrogenase mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-(2H7)-Flow-Cytometry-NBP2-22125-img0004.jpg)
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis using Glucose 6 Phosphate Dehydrogenase mAb against human Glucose 6 Phosphate Dehydrogenase (AA: 275-515) recombinant protein. (Expected MW is 53.1 kDa)](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-(2H7)-Western-Blot-NBP2-22125-img0002.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Analysis of paraffin-embedded breast cancer tissues using Glucose 6 Phosphate Dehydrogenase mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-(2H7)-Immunohistochemistry-Paraffin-NBP2-22125-img0005.jpg)
![ELISA: Glucose 6 Phosphate Dehydrogenase Antibody (2H7) [NBP2-22125] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-(2H7)-ELISA-NBP2-22125-img0001.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Analysis in human testis and skeletal muscle tissues. Corresponding G6PD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89804-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Analysis in human testis and skeletal muscle tissues. Corresponding G6PD RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Analysis in human cell lines A-549 and HEK293 using anti-G6PD antibody. Corresponding G6PD RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NBP1-89804-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Analysis using Anti-G6PD antibody NBP1-89804 (A) shows similar pattern to independent antibody NBP1-89805 (B).](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NBP1-89804-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human cell line U-2 OS shows positivity in cytoplasm. Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89804-img0012.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89804-img0021.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89804-img0017.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human spleen shows moderate cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89804-img0018.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human colon shows moderate cytoplasmic positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89804-img0019.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89804] - Staining of human liver shows moderate to strong cytoplasmic positivity in Kupffer cells.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89804-img0020.jpg)
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Analysis using Anti-G6PD antibody NBP1-89805 (A) shows similar pattern to independent antibody NBP1-89804 (B).](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NBP1-89805-img0021.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Analysis using Anti-G6PD antibody NBP1-89805 (A) shows similar pattern to independent antibody NBP1-89804 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89805-img0006.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89805-img0020.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Analysis in human cell lines A-549 and HEK293 using anti-G6PD antibody. Corresponding G6PD RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NBP1-89805-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Staining in human testis and skeletal muscle tissues.. Corresponding G6PD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89805-img0016.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89805-img0017.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NBP1-89805] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-89805-img0018.jpg)
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954] - Analysis of Human Testis lysate (35ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Western-Blot-NB100-60954-img0006.jpg "Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954] - Analysis of Human Testis lysate (35ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954] - Staining of paraffin embedded Human Spleen. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NB100-60954-img0008.jpg)
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-60954] - Staining of paraffin embedded Human Testis. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NB100-60954-img0005.jpg)
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (S02-6B6) [NBP3-14935] - Western blot detection of Glucose 6 Phosphate Dehydrogenase in K562, C6, Hela cell lysates using Glucose 6 Phosphate Dehydrogenase Rabbit mAb (1:1000 diluted). Predicted band size: 59kDa. Observed band size: 59kDa.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-(S02-6B6)-Western-Blot-NBP3-14935-img0001.jpg "Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (S02-6B6) [NBP3-14935] - Western blot detection of Glucose 6 Phosphate Dehydrogenase in K562, C6, Hela cell lysates using Glucose 6 Phosphate Dehydrogenase Rabbit mAb (1:1000 diluted). Predicted band size: 59kDa. Observed band size: 59kDa.")
![Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (S02-6B6) [NBP3-14935] - Immunohistochemistry of Glucose 6 Phosphate Dehydrogenase in paraffin-embedded Human tonsil using Glucose 6 Phosphate Dehydrogenase Rabbit mAb at dilution 1/100](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-(S02-6B6)-Immunohistochemistry-Paraffin-NBP3-14935-img0002.jpg)
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2F6) [NBP1-30358] - Lane 1. MCF cell lysate, Lane 2. HeLa cell lysate, Lane 3. Jurkat cell lysate.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-(2F6)-Western-Blot-NBP1-30358-img0004.jpg "Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (2F6) [NBP1-30358] - Lane 1. MCF cell lysate, Lane 2. HeLa cell lysate, Lane 3. Jurkat cell lysate.")
![Immunocytochemistry/Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody (2F6) [NBP1-30358] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human G6PD antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-(2F6)-Immunocytochemistry-Immunofluorescence-NBP1-30358-img0005.jpg)
![SDS-Page: Recombinant Human Glucose 6 Phosphate Dehydrogenase Protein [NBP1-98994] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize2/Recombinant-Human-Glucose-6-Phosphate-Dehydrogenase-Protein-SDS-Page-NBP1-98994-img0001.jpg "SDS-Page: Recombinant Human Glucose 6 Phosphate Dehydrogenase Protein [NBP1-98994] - 15% SDS-PAGE (3ug)")
![Western Blot: Glucose 6 Phosphate Dehydrogenase Protein [NBC1-19064] - 55.7kDa (491 aa) on SDS - PAGE](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Protein-Western-Blot-NBC1-19064-img0001.jpg "Western Blot: Glucose 6 Phosphate Dehydrogenase Protein [NBC1-19064] - 55.7kDa (491 aa) on SDS - PAGE")
![Western Blot: Glucose 6 Phosphate Dehydrogenase Overexpression Lysate (Adult Normal) [NBL1-10899] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glucose 6 Phosphate Dehydrogenase.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBL1-10899-img0002.jpg "Western Blot: Glucose 6 Phosphate Dehydrogenase Overexpression Lysate (Adult Normal) [NBL1-10899] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glucose 6 Phosphate Dehydrogenase.")
![Western Blot: Glucose 6 Phosphate Dehydrogenase Overexpression Lysate (Adult Normal) [NBL1-10900] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glucose 6 Phosphate Dehydrogenase.](http://images.novusbio.com/fullsize/Glucose-6-Phosphate-Dehydrogenase-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBL1-10900-img0002.jpg "Western Blot: Glucose 6 Phosphate Dehydrogenase Overexpression Lysate (Adult Normal) [NBL1-10900] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glucose 6 Phosphate Dehydrogenase.")
![Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (6V8O9) [NBP3-15359] - Western blot analysis of extracts of various cell lines, using Glucose 6 Phosphate Dehydrogenase Rabbit mAb (NBP3-15359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize2/Glucose-6-Phosphate-Dehydrogenase-Antibody-(6V8O9)-Western-Blot-NBP3-15359-img0001.jpg "Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (6V8O9) [NBP3-15359] - Western blot analysis of extracts of various cell lines, using Glucose 6 Phosphate Dehydrogenase Rabbit mAb (NBP3-15359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")