MAB8844) coated on a Clear Polystyrene Microplate (
DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994)." class="big_lightbox">

MAB8844) coated on a Clear Polystyrene Microplate (
DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994)." alt="Recombinant Human APRIL/TNFSF13 protein was serially diluted 2-fold and captured by Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (
MAB8844) coated on a Clear Polystyrene Microplate (
DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994)." class="big_thumb" />
AB-108-C, open histogram) followed by anti-Goat IgG PE-conjugated Secondary Antibody (
F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (
FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

AB-108-C, open histogram) followed by anti-Goat IgG PE-conjugated Secondary Antibody (
F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (
FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />