| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02957-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4037-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54771-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26391-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26392-50ul | |||
![Western Blot: Smad2 Antibody [NBP3-02957] - Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (NBP3-02957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.](http://images.novusbio.com/fullsize/Smad2-Antibody-Western-Blot-NBP3-02957-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Smad2 Antibody [NBP3-02957] - Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (NBP3-02957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.")
![Western Blot: Smad2 [p Ser467] Antibody [NBP2-54771] - Analysis of lysed extracts from HeLa cells untreated or treated with PMA using Smad2 (Phospho-Ser467).](http://images.novusbio.com/fullsize/Smad2-[p-Ser467]-Antibody-Western-Blot-NBP2-54771-img0001.jpg "Western Blot: Smad2 [p Ser467] Antibody [NBP2-54771] - Analysis of lysed extracts from HeLa cells untreated or treated with PMA using Smad2 (Phospho-Ser467).")
![Western Blot: Smad2 [p Ser250] Antibody (S02-0D1) [NBP3-19828] - Western blot detection of Smad2 (phospho-Ser250) in Hela, CHO-K1, C6 using NBP3-19828 (1:1000 diluted)](http://images.novusbio.com/fullsize/Smad2-p-Ser250-Antibody-S02-0D1-Western-Blot-NBP3-19828-img0001.jpg "Western Blot: Smad2 [p Ser250] Antibody (S02-0D1) [NBP3-19828] - Western blot detection of Smad2 (phospho-Ser250) in Hela, CHO-K1, C6 using NBP3-19828 (1:1000 diluted)")
![Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP2-44217] - Lane 1: MDA-MB-231 cells. Lane 2: MDA-MB-231 cells treated with TGF-beta1 for 1h. Load: 20 Ug per lane.](http://images.novusbio.com/fullsize/Smad2-3-[p-Ser-465]-Antibody-Western-Blot-NBP2-44217-img0001.jpg "Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP2-44217] - Lane 1: MDA-MB-231 cells. Lane 2: MDA-MB-231 cells treated with TGF-beta1 for 1h. Load: 20 Ug per lane.")
![Western Blot: Smad2 [p Ser255, p Ser245, p Ser250] Antibody [NBP3-13246] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad2 (phospho Ser245/Ser250/Ser255) antibody (NBP3-13246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Smad2-[p-Ser255--p-Ser245--p-Ser250]-Antibody-Western-Blot-NBP3-13246-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Smad2 [p Ser255, p Ser245, p Ser250] Antibody [NBP3-13246] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad2 (phospho Ser245/Ser250/Ser255) antibody (NBP3-13246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![ELISA: Rat Smad2 ELISA Kit (Colorimetric) [NBP2-76619] - Samples were spiked with high concentrations of Rat Smad2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76619_rat-smad2-elisa-kit-colorimetric-132202416215363.jpg "ELISA: Rat Smad2 ELISA Kit (Colorimetric) [NBP2-76619] - Samples were spiked with high concentrations of Rat Smad2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Smad2 ELISA Kit (Colorimetric) [NBP2-76619] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76619_rat-smad2-elisa-kit-colorimetric-432024932652.jpg)
![ELISA: Human Smad2 ELISA Kit (Colorimetric) [NBP2-76620] - Samples were spiked with high concentrations of Human Smad2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76620_human-smad2-elisa-kit-colorimetric-132202416215311.jpg "ELISA: Human Smad2 ELISA Kit (Colorimetric) [NBP2-76620] - Samples were spiked with high concentrations of Human Smad2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Smad2 ELISA Kit (Colorimetric) [NBP2-76620] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76620_human-smad2-elisa-kit-colorimetric-4320249151649.jpg)
![Western Blot: SMAD2 Overexpression Lysate (Adult Normal) [NBL1-16220] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMAD2.](http://images.novusbio.com/fullsize/SMAD2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16220-img0002.jpg "Western Blot: SMAD2 Overexpression Lysate (Adult Normal) [NBL1-16220] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMAD2.")
![Western Blot: SMAD2 Overexpression Lysate (Adult Normal) [NBL1-16221] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMAD2.](http://images.novusbio.com/fullsize/SMAD2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16221-img0002.jpg "Western Blot: SMAD2 Overexpression Lysate (Adult Normal) [NBL1-16221] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMAD2.")
![Immunocytochemistry/Immunofluorescence: Smad2 [p Ser255] Antibody (2A12) [NBP3-26391] - Staining of A549 cells with Smad2 [p Ser255] Antibody (2A12) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26391_rabbit-smad2-p-ser-255-mab-2a12-282202412475143.jpg "Immunocytochemistry/Immunofluorescence: Smad2 [p Ser255] Antibody (2A12) [NBP3-26391] - Staining of A549 cells with Smad2 [p Ser255] Antibody (2A12) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).")
![Western Blot: Smad2 [p Ser250] Antibody (4D12) [NBP3-26392] - Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate. All lanes: Smad2 [p Ser250] Antibody at 1.07ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58 KDa Observed band size: 58 KDa](http://images.novusbio.com/fullsize/nbp3-26392_rabbit-smad2-p-ser-250-mab-4d12-282202411413615.jpg "Western Blot: Smad2 [p Ser250] Antibody (4D12) [NBP3-26392] - Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate. All lanes: Smad2 [p Ser250] Antibody at 1.07ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58 KDa Observed band size: 58 KDa")