NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Syndecan-4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Syndecan-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2918) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2918-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90700-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90173-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50315-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90701-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90699-50ul | |||
![Western Blot: Syndecan-4 Antibody [NBP2-24630] - Analysis of Syndecan 4 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) Raw cell lysate using NBP2-24630 at 1.0 ug/ml.](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-Western-Blot-NBP2-24630-img0003.jpg "Western Blot: Syndecan-4 Antibody [NBP2-24630] - Analysis of Syndecan 4 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) Raw cell lysate using NBP2-24630 at 1.0 ug/ml.")
![ELISA: Mouse Syndecan-4 ELISA Kit (Colorimetric) [NBP2-76612] - Samples were spiked with high concentrations of Mouse Syndecan-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76612_mouse-syndecan-4-elisa-kit-colorimetric-132202416204098.jpg "ELISA: Mouse Syndecan-4 ELISA Kit (Colorimetric) [NBP2-76612] - Samples were spiked with high concentrations of Mouse Syndecan-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Syndecan-4 ELISA Kit (Colorimetric) [NBP2-76612] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76612_mouse-syndecan-4-elisa-kit-colorimetric-4320248582588.jpg)
![Immunohistochemistry-Paraffin: Syndecan-4 Antibody (047) [NBP2-90700] - Staining of mouse SDC4 in mouse intestine with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-047-Immunohistochemistry-Paraffin-NBP2-90700-img0003.jpg "Immunohistochemistry-Paraffin: Syndecan-4 Antibody (047) [NBP2-90700] - Staining of mouse SDC4 in mouse intestine with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: Syndecan-4 Antibody (047) [NBP2-90700] - Staining of mouse SDC4 in mouse stomach with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-047-Immunohistochemistry-Paraffin-NBP2-90700-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Syndecan-4 Antibody (047) [NBP2-90700] - Immunofluorescence staining of Syndecan-4 in mouse splenocytes. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90700 (1:60) at 4 overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-90700_rabbit-monoclonal-syndecan-4-antibody-047-235202514331535.png)
![N/A Syndecan-4 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Syndecan4_DY2918_ELISA_1896.jpg "N/A Syndecan-4 [Biotin]")
![Immunohistochemistry-Paraffin: Syndecan-4 Antibody [NBP1-90173] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90173-img0005.jpg "Immunohistochemistry-Paraffin: Syndecan-4 Antibody [NBP1-90173] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.")
![Western Blot: Syndecan-4 Antibody (V60P2C2/F2) [NBP2-50315] - Analysis of Syndecan-4 in Hela cell lysate.](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-V60P2C2-F2-Western-Blot-NBP2-50315-img0001.jpg "Western Blot: Syndecan-4 Antibody (V60P2C2/F2) [NBP2-50315] - Analysis of Syndecan-4 in Hela cell lysate.")
![Western Blot: Syndecan-4 Antibody (112) [NBP2-90701] - Lane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-112-Western-Blot-NBP2-90701-img0003.jpg "Western Blot: Syndecan-4 Antibody (112) [NBP2-90701] - Lane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa")
![Flow Cytometry: Syndecan-4 Antibody (112) [NBP2-90701] - Analysis of Mouse SDC4 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse SDC4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-112-Flow-Cytometry-NBP2-90701-img0002.jpg)
![ELISA: Rat Syndecan-4 ELISA Kit (Colorimetric) [NBP2-76613] - Samples were spiked with high concentrations of Rat Syndecan-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76613_rat-syndecan-4-elisa-kit-colorimetric-132202416221034.jpg "ELISA: Rat Syndecan-4 ELISA Kit (Colorimetric) [NBP2-76613] - Samples were spiked with high concentrations of Rat Syndecan-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Syndecan-4 ELISA Kit (Colorimetric) [NBP2-76613] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76613_rat-syndecan-4-elisa-kit-colorimetric-432024903535.jpg)
![Western Blot: Syndecan 4 Overexpression Lysate (Adult Normal) [NBP2-04238] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Syndecan 4.](http://images.novusbio.com/fullsize/Syndecan-4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04238-img0001.jpg "Western Blot: Syndecan 4 Overexpression Lysate (Adult Normal) [NBP2-04238] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Syndecan 4.")
