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![Immunocytochemistry/Immunofluorescence: Syndecan-4 Antibody (008) [NBP2-90699] - Immunofluorescence staining of mSDC4 in mouse spleen cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-mouse mSDC4 monoclonal antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to .](http://images.novusbio.com/fullsize/Syndecan-4-Antibody-008-Immunocytochemistry-Immunofluorescence-NBP2-90699-img0001.jpg "Immunocytochemistry/Immunofluorescence: Syndecan-4 Antibody (008) [NBP2-90699] - Immunofluorescence staining of mSDC4 in mouse spleen cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-mouse mSDC4 monoclonal antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to .")
![Western Blot: Syndecan-4 Antibody (PSH0-72) [NBP3-32987] - Western blot analysis of Syndecan-4 on different lysates with Rabbit anti-Syndecan-4 antibody (NBP3-32987) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: U-87 MG cell lysate Lane 5: A549 cell lysate Lane 6: MCF7 cell lysate Lane 7: MDA-MB-231 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32987) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32987_rabbit-syndecan-4-mab-psh0-72-288202412493463.jpg "Western Blot: Syndecan-4 Antibody (PSH0-72) [NBP3-32987] - Western blot analysis of Syndecan-4 on different lysates with Rabbit anti-Syndecan-4 antibody (NBP3-32987) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: U-87 MG cell lysate Lane 5: A549 cell lysate Lane 6: MCF7 cell lysate Lane 7: MDA-MB-231 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32987) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![SDS-Page: Recombinant Human Syndecan-4 Protein [NBP1-72277] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-Syndecan-4-Protein-SDS-Page-NBP1-72277-img0001.jpg "SDS-Page: Recombinant Human Syndecan-4 Protein [NBP1-72277] - 15% SDS-PAGE (3ug)")