| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22205-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8965-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-82120-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP3-11951-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP3-11950-25ul | |||
![Western Blot: p70 S6 Kinase/S6K Antibody [NBP2-38448] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-Western-Blot-NBP2-38448-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p70 S6 Kinase/S6K Antibody [NBP2-38448] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K Antibody [NBP2-38448] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38448-img0004.jpg)
![Immunohistochemistry: p70 S6 Kinase/S6K Antibody [NBP2-38448] - Staining of human bone marrow shows distinct nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-Immunohistochemistry-NBP2-38448-img0002.jpg)
![Western Blot: p70 S6 Kinase/S6K Antibody [NBP2-38448] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-Western-Blot-NBP2-38448-img0003.jpg)
![Western Blot: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Western blot analysis using S6K S6K mAb against human RPS6KB1 (AA: 295-524) recombinant protein. (Expected MW is 59 kDa)](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-5G9-Western-Blot-NBP2-22205-img0005.jpg "Western Blot: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Western blot analysis using S6K S6K mAb against human RPS6KB1 (AA: 295-524) recombinant protein. (Expected MW is 59 kDa)")
![Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Immunohistochemical analysis of paraffin-embedded muscle tissues using S6K mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-5G9-Immunohistochemistry-Paraffin-NBP2-22205-img0003.jpg)
![Flow Cytometry: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Flow cytometric analysis of Jurkat cells using S6K mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-5G9-Flow-Cytometry-NBP2-22205-img0001.jpg)
![Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using S6K mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-5G9-Immunohistochemistry-Paraffin-NBP2-22205-img0002.jpg)
![ELISA: p70 S6 Kinase/S6K Antibody (5G9) [NBP2-22205] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-Antibody-5G9-ELISA-NBP2-22205-img0004.jpg)
![Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using NB100-82120 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Ser411]-Antibody-Immunohistochemistry-NB100-82120-img0006.jpg "Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using NB100-82120 (left) or the same antibody preincubated with blocking peptide(right).")
![<b>Biological Strategies Validation. </b>Western Blot: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Analysis of extracts from MCF cells untreated or treated with EGF using NB100-82120](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Ser411]-Antibody-Western-Blot-NB100-82120-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K [p Ser411] Antibody [NB100-82120] - Staining of methanol-fixed MCF7 cells using NB100-82120.](http://images.novusbio.com/fullsize/nb100-82120_rabbit-polyclonal-p70-s6-kinase-s6k-p-ser411-antibody-11320251555919.jpg)
![Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32783) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32783_rabbit-p70-s6-kinase-s6k-p-thr389-mab-psh02-23-29820241492679.jpg "Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32783) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Immunocytochemistry analysis of MCF7 cells treated with or without 20% FBS overnight then add 100nM Calyculin A for 30 minutes labeling p70 S6 Kinase/S6K with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32783_rabbit-p70-s6-kinase-s6k-p-thr389-mab-psh02-23-292024111483.jpg)
![Western Blot: p70 S6 Kinase/S6K [p Thr389] Antibody (PSH02-23) [NBP3-32783] - Western blot analysis of p70 S6 Kinase/S6K on different lysates with Rabbit anti-p70 S6 Kinase/S6K antibody (NBP3-32783) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MCF7 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 20% FBS overnight then add 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 59 kDa Observed band size: 70/85 kDa Exposure time: Lane 1-4 (left): 1 minute 2 seconds; Lane 1-4 (right): 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32783_rabbit-p70-s6-kinase-s6k-p-thr389-mab-psh02-23-3920248453540.jpg)
![Western Blot: p70 S6 Kinase/S6K [p Thr 449] Antibody [NBP3-11951] - Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53 kDa p70 S6 Kinase/S6K phosphorylated at Thr449 in the first lane (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide in the third lane (NP).](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-Thr-449]-Antibody-Western-Blot-NBP3-11951-img0001.jpg "Western Blot: p70 S6 Kinase/S6K [p Thr 449] Antibody [NBP3-11951] - Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53 kDa p70 S6 Kinase/S6K phosphorylated at Thr449 in the first lane (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide in the third lane (NP).")
![Western Blot: p70 S6 Kinase/S6K [p ser 398] Antibody [NBP3-11950] - Western blot of Drosophila S2 cell lysate showing specific labeling of the ~70 kDa p70 S6 Kinase/S6K protein phosphorylated at Thr398 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).](http://images.novusbio.com/fullsize/p70-S6-Kinase-S6K-[p-ser-398]-Antibody-Western-Blot-NBP3-11950-img0001.jpg "Western Blot: p70 S6 Kinase/S6K [p ser 398] Antibody [NBP3-11950] - Western blot of Drosophila S2 cell lysate showing specific labeling of the ~70 kDa p70 S6 Kinase/S6K protein phosphorylated at Thr398 in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).")
![Western Blot: S6K Overexpression Lysate (Adult Normal) [NBL1-15574] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for S6K.](http://images.novusbio.com/fullsize/S6K-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15574-img0002.jpg "Western Blot: S6K Overexpression Lysate (Adult Normal) [NBL1-15574] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for S6K.")
![SDS-Page: Recombinant Human p70 S6 Kinase/S6K Isoform a Protein [NBP2-61989] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-p70-S6-Kinase-S6K-Isoform-a-Protein-SDS-Page-NBP2-61989-img0002.jpg "SDS-Page: Recombinant Human p70 S6 Kinase/S6K Isoform a Protein [NBP2-61989] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")