Periostin/OSF-2: Novus Biologicals

NBP1-30042

Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free

(2 Reviews)

(38 Publications)

0.1 ml

NBP1-30042

Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Analysis of periostin in rabbit periosteum. Image courtesy of product review submitted by Samer Zaky.

Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.

Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Western-Blot-NBP1-30042-img0007.jpg

Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-30042-img0006.jpg

<b>Genetic Strategies Validation. </b>Periostin-OSF-2 Antibody - C-terminus - Azide and BSA Free-Immunohistochemistry-NBP1-30042-img0010.jpg

<b>Genetic Strategies Validation. </b>Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Knockout-Validated-NBP1-30042-img0005.jpg

$Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age.(A) Evaluation of cardiac function by transthoracic echocardiographic analyses. Left ventricular ejection fractions (LVEFs) are shown (n = 8–9). (B) H&E staining of cryosections from cardiac muscle. (C) Immunostaining of laminin ɑ2 (green) & periostin (red) with DAPI (blue) from cardiac muscle. (D) Western blot analyses of periostin. The graph shows the quantification of periostin levels normalized to GAPDH (n = 3). (E) qPCR analyses of Tgfb2 (TGF beta 2), Postn (periostin), & Fn1 (fibronectin) mRNA levels in cardiac muscle (n = 4). (F) qPCR analyses of Nppa (natriuretic peptide A) & Nppb (natriuretic peptide B) mRNA levels in cardiac muscle (n = 4). In qPCR analyses, data were normalized by Gapdh mRNA & expressed as fold increases from WT mice. ***P<0.001 compared with WT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31430335), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - TGFBI & periostin are induced in the heart after injury.(A) Western blot analysis of periostin & TGFBI in isolated adult cardiomyocytes & fibroblasts. Sarcomeric alpha -actin was used as a control for cardiomyocyte purity & GAPDH was used as a loading control. (B) Quantitative real time PCR for Postn & Tgfbi from 1 week sham or MI-operated hearts. mRNA levels were normalized to 18s ribosomal RNA. *p<0.05 for both genes in MI-operated animals compared to sham animals using an unpaired Student’s T-test. n = 3 animals. (C) Western blot analysis for periostin & TGFBI in the infarcted areas isolated from hearts 24 hours, 7 & 14 days after MI surgery. GAPDH was used as a loading control. Each lane corresponds to protein from one mouse. (D & E) Quantification of TGFBI (D) & periostin (E) protein levels from the conditions shown in panel C except that 3 hearts were analyzed in total each. (F) Immunohistochemistry for the indicated markers/proteins on sham or MI-operated hearts after 1 week. Images were taken at 400x magnification. Scale = 20 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0181945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - PostnMCM allele activity in vivo.(a) Schematic representation of the Postn genetic locus with a tamoxifen-regulated MerCreMer cDNA cassette inserted into exon 1 (E1), which was crossed with Rosa26 reporter mice (R26-eGFP) containing loxP sites flanking a stop cassette upstream of eGFP to allow for Cre-dependent lineage tracing. (b) Experimental scheme whereby PostnMCM/+; R26-eGFP mice were given tamoxifen for 8 weeks before harvesting at 16 weeks. (c) Representative histological section from the heart of mice described in a & b, which show exceptionally rare labelling of interstitial cells at baseline (arrow) with 8 weeks of tamoxifen. Nuclei are stained in blue. Inset shows alpha -actin stained cardiomyocytes (red) surrounding the one eGFP-labelled interstitial cell (green) (n=4 mice). (d) Experimental scheme whereby PostnMCM/+; R26-eGFP mice were MI injured or subjected to a sham procedure, then given tamoxifen for 1 week before harvesting. (e) Western blot analysis for periostin, eGFP, Cre (MerCreMer protein) & GAPDH as a control at day 0 before injury or day 7 after MI injury with 1 week of tamoxifen (n=3 mice per condition). (f) Representative histological sections showing eGFP-labelled interstitial cells in hearts of PostnMCM/+; R26-eGFP after MI injury with 7 days of tamoxifen labelling, but not with a sham procedure (n=6 mice for MI & n=3 for sham). (g) Whole-mount fluorescent images of hearts from PostnMCM/+; R26-eGFP mice for direct eGFP fluorescence over the given time course shown. A no tamoxifen control 7 days after MI is also shown (n=3 mice per time point & condition). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27447449), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ch, Bv, Ca, Eq, Fe, Av-Bi, Gt, Gp, Ha, Pm, Rb, Sh, Vo

Species Glossary

Reactivity: Hu, Mu, Rt, Ch, Bv, Ca, Eq, Fe, Av-Bi, Gt, Gp, Ha, Pm, Rb, Sh, Vo

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP1-30042SS

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AF2955

Periostin/OSF-2 Antibody [Unconjugated]

(1 Review)

(32 Publications)

100 μg

AF2955

2955-F2), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Periostin/OSF-2 (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Periostin/ OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox">

Recombinant Mouse Periostin/OSF-2 (<a class=

2955-F2), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Periostin/OSF-2 (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Periostin/ OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955). The ND₅₀ is typically 1-5 µg/mL." alt="Recombinant Mouse Periostin/OSF-2 (2955-F2), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Periostin/OSF-2 (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Periostin/ OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Periostin/OSF-2 was detected in immersion fixed rat mesenchymal stem cells differentiated to osteoblasts using Goat Anti-Mouse Periostin/OSF-2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

AAV8-MD1 delivery significantly reduces collagen VI and periostin expression in the diaphragm of D2.mdx mice. Male Dba2/J control and D2.mdx mice were treated with a single IV injection of either saline or AAV8-MD1 at 4 x 1012 vg/mouse at 6 weeks of age. (A) Representative images of collagen VI deposition in mouse diaphragms for each treatment group are shown as whole diaphragm sections (top row; scale bar: 500 µm) as well as a random close-up of each section (bottom row; scale bar: 100 µm). (B) Collagen VI-positive area within an entire diaphragm section was quantified and expressed as percentage of the total diaphragm section area. (C) Western blot analysis displaying periostin protein expression and (D) subsequent quantification of the level of periostin, normalized to alpha -tubulin, n = 4–5. In (B,D), data shown as ± SEM, n = 4–5. Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple comparisons test: p < 0.0001 (****), p < 0.001 (***), p < 0.001 (**), ns: not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37175881), licensed under a CC-BY license. Not internally tested by R&D Systems.

Analysis of the heterogeneity of the fibroblast subpopulations.(A) t-SNE plot of the 10 cardiac fibroblast subpopulations in the high-fat diet (HFD)/streptozotocin (STZ)-treated and control mice (n = 3428 fibroblasts from healthy control and n = 2988 fibroblasts from 6 diabetic mice). (B) Correlation heatmap of the gene-expression signatures of each fibroblast subpopulation. Color differences indicate subpopulations that were resolved through multiscale bootstrapping. (C) Heatmap showing the top 5 marker genes in each fibroblast subpopulation. The red color indicates high expression, while green color indicates low expression. (D) Heatmap showing the enriched Gene Ontology (GO) terms associated with cardiac fibrosis in each fibroblast population. (E, F) Representative immunofluorescence images of Hrc (E) and Postn (F) in mouse heart (n = 6 mice per group), scale bar = 100 µm. The details of the 10 transcriptionally distinct fibroblast subpopulations are listed in Supplementary file 15. The details of distinct signatures of each fibroblast subpopulation in the heart are listed in Supplementary file 16. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37010266), licensed under a CC-BY license. Not internally tested by R&D Systems.

vivoPMO significantly reduced mPostn exon 17 expression and induced beneficial effect on muscles. RT-qPCR analysis of e17+ (A) and total (B) Postn mRNA expression represented as fold change compared to wild type (N = 4–6). (C) Full-length protein expression assessment using Western blot (D) analysis. (E) DIA hydroxyproline analysis (indicative of collagen level). (F) Forelimb grip strength was measured as the average of 5 discrete measurements of maximum force normalised to body weight. Outliers were identified via the ROUT method and omitted from the statistical analysis. The data were analysed by one-way ANOVA with Tukey post hoc tests and significance was recorded as * = p < 0.05; ** = p < 0.01; **** = p < 0.0001. Error bars = +/−SD; qPCR was performed in triplicate. Legend: ‘wt’ = Dba.2J wild type; ‘mdx’ = D2.mdx + Scramble vivoPMO; ‘mdx + pmo’ = D2.mdx + Postn exon 17 skipping vivoPMO. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/25/11/6113), licensed under a CC-BY license. Not internally tested by R&D Systems.

Oim−/− mice display a severe PDL phenotype which can be partially improved by alendronate treatment. Representative images of Sirius red staining (A), immunofluorescent staining of periostin (B) and FITC staining (C). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39747677), licensed under a CC-BY license. Not internally tested by R&D Systems.

2955-F2) was serially diluted and captured by Rat Anti-Mouse Periostin/OSF‑2 Monoclonal Antibody (Catalog # MAB2955) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Periostin/OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse Periostin/OSF‑2 (Catalog # <a class=

2955-F2) was serially diluted and captured by Rat Anti-Mouse Periostin/OSF‑2 Monoclonal Antibody (Catalog # MAB2955) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Periostin/OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2955U-100UG
Alexa Fluor 405	AF2955V-100UG
Alexa Fluor 488	AF2955G-100UG
Alexa Fluor 532	AF2955X-100UG
Alexa Fluor 594	AF2955T-100UG
Alexa Fluor 647	AF2955R-100UG
Alexa Fluor 700	AF2955N-100UG
Alexa Fluor 750	AF2955S-100UG
Alexa Fluor Plus 405	AF2955AFP405-100UG
Alexa Fluor Plus 488	AF2955AFP488-100UG
Alexa Fluor Plus 555	AF2955AFP555-100UG
Alexa Fluor Plus 594	AF2955AFP594-100UG
Alexa Fluor Plus 647	AF2955AFP647-100UG
Alexa Fluor Plus 680	AF2955AFP680-100UG
Allophycocyanin	AF2955A
Biotin	BAF2955
CoraFluor 1	AF2955CL1
DyLight 350	AF2955D
DyLight 405	AF2955E
DyLight 488	AF2955K
DyLight 550	AF2955L
DyLight 594	AF2955M
DyLight 650	AF2955W
DyLight 680	AF2955Y
DyLight 755	AF2955Z
FITC	AF2955F
HRP	AF2955H
Janelia Fluor 525	AF2955JF525
Janelia Fluor 549	AF2955I
Janelia Fluor 646	AF2955J
Janelia Fluor 669	AF2955JF669
PE	AF2955P
PerCP	AF2955C
mFluor Violet 450 SE	AF2955MFV450
mFluor Violet 500 SE	AF2955MFV500
mFluor Violet 610 SE	AF2955MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2955-SP

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3548-F2

Recombinant Human Periostin/OSF-2 Protein, CF

(32 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP1-82472

Periostin/OSF-2 Antibody - BSA Free

(8 Publications)

0.1 ml

NBP1-82472

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining of human pancreas shows low expression as expected.

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody [NBP1-82472] - Staining of human skin shows high expression.

<b>Genetic Strategies Validation. </b>Periostin-OSF-2-Antibody-Knockdown-Validated-NBP1-82472-img0009.jpg

nbp1-82472_rabbit-periostin-osf-2-pab-20920231742123.jpg

Serial sections of non-small cell lung carcinoma (NSCLC) immunostained for (A) periostin (POSTN), (B) alpha -smooth muscle actin ( alpha -SMA), (C) podoplanin (D2-40), and (D) vimentin in cancer-associated fibroblasts (CAFs). Magnification 50×. Bar = 200 µm.

Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] - The efficacy of shRNA lentiviral particles transfection. (A) Silencing of periostin (POSTN) expression was confirmed by Western blot analysis. beta -actin was used as an internal control. (B) Confocal images & fluorescence analysis showing the expression of POSTN in control A549.CTRL cells & A549.shRNA cells (*** p < 0.001). Objective 60×/1.40 Oil; pinhole airy 1.25. Bar = 20 µm. (C) The expression of POSTN mRNA in control cells (A549.CTRL) & the cells representing the loss-of-function phenotype (A549.shRNA). Relative expression (RQ) of the POSTN gene was normalized against the expression of ACTB (*** p < 0.001). RQ, relative quantification. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35163164), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Periostin/OSF-2 Antibody [NBP1-82472] - Characteristics of lung cancer cell lines according to periostin (POSTN) expression. (A) Comparison of POSTN expression levels detected by Western blot & (B) by confocal microscopy (fluorescence intensity) in different lung cancer cell lines (A549, NCI-H522, NCI-H1703), (p > 0.05). Objective 60×/1.40 Oil; pinhole airy 1.25. Bar = 20 µm. (C) Immunohistochemical expression level of periostin (POSTN) in the tissue of particular non-small cell lung carcinoma (NSCLC) subtypes: squamous cell carcinoma (SCC) & adenocarcinoma (AC) (p > 0.05). IRS, immunoreactive score. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35163164), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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2955-F2

Recombinant Mouse Periostin/OSF-2 Protein, CF

(19 Publications)

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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MAB3548

Periostin/OSF-2 Antibody (345613) [Unconjugated]

(11 Publications)

100 μg

MAB3548

Periostin/OSF-2 was detected in immersion fixed human osteocytes using Rat Anti-Human/Mouse Periostin/OSF-2 Monoclonal Antibody (Catalog # MAB3548) at 10 µg/mL for 3 hours at room temperature. Cells were stained red and counterstained with DAPI (blue).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3548U-100UG
Alexa Fluor 405	FAB3548V-100UG
Alexa Fluor 488	FAB3548G-100UG
Alexa Fluor 532	FAB3548X-100UG
Alexa Fluor 594	FAB3548T-100UG
Alexa Fluor 647	FAB3548R-100UG
Alexa Fluor 700	FAB3548N-100UG
Alexa Fluor 750	FAB3548S-100UG
Alexa Fluor Plus 405	FAB3548AFP405-100UG
Alexa Fluor Plus 488	FAB3548AFP488-100UG
Alexa Fluor Plus 555	FAB3548AFP555-100UG
Alexa Fluor Plus 594	FAB3548AFP594-100UG
Alexa Fluor Plus 647	FAB3548AFP647-100UG
Alexa Fluor Plus 680	FAB3548AFP680-100UG
Allophycocyanin	FAB3548A
Biotin	FAB3548B
CoraFluor 1	FAB3548CL1
DyLight 350	FAB3548D
DyLight 405	FAB3548E
DyLight 488	FAB3548K
DyLight 550	FAB3548L
DyLight 594	FAB3548M
DyLight 650	FAB3548W
DyLight 680	FAB3548Y
DyLight 755	FAB3548Z
FITC	FAB3548F
HRP	FAB3548H
Janelia Fluor 525	FAB3548JF525
Janelia Fluor 549	FAB3548I
Janelia Fluor 646	FAB3548J
Janelia Fluor 669	FAB3548JF669
PE	FAB3548P
PerCP	FAB3548C
mFluor Violet 450 SE	FAB3548MFV450
mFluor Violet 500 SE	FAB3548MFV500
mFluor Violet 610 SE	FAB3548MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3548-SP

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AF3548

Periostin/OSF-2 Antibody [Unconjugated]

(7 Publications)

100 μg

AF3548

DYC002) and incubated with 3 µg Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) or Normal Goat IgG Control (AB-108-C) plus 30 µL Protein G beads overnight. Immunoprecipitated Periostin/OSF-2 was detected by Western blot under reducing conditions using 1 µg/mL Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548). View our recommended buffer recipes for immunoprecipitation." class="big_lightbox">

Human Periostin/OSF-2 was immunoprecipitated from human milk samples diluted in 1X Sample Diluent Concentrate 2 (<a class=

DYC002) and incubated with 3 µg Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) or Normal Goat IgG Control (AB-108-C) plus 30 µL Protein G beads overnight. Immunoprecipitated Periostin/OSF-2 was detected by Western blot under reducing conditions using 1 µg/mL Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548). View our recommended buffer recipes for immunoprecipitation." alt="Human Periostin/OSF-2 was immunoprecipitated from human milk samples diluted in 1X Sample Diluent Concentrate 2 (DYC002) and incubated with 3 µg Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) or Normal Goat IgG Control (AB-108-C) plus 30 µL Protein G beads overnight. Immunoprecipitated Periostin/OSF-2 was detected by Western blot under reducing conditions using 1 µg/mL Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548). View our recommended buffer recipes for immunoprecipitation." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Periostin/OSF-2 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Periostin/OSF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF017). A specific band was detected for Periostin/OSF‑2 at approximately ~75kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of Human Breast Cancer. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Periostin/OSF‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3548) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Periostin/OSF‑2 at approximately ~75kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3548U-100UG
Alexa Fluor 405	AF3548V-100UG
Alexa Fluor 488	AF3548G-100UG
Alexa Fluor 532	AF3548X-100UG
Alexa Fluor 594	AF3548T-100UG
Alexa Fluor 647	AF3548R-100UG
Alexa Fluor 700	AF3548N-100UG
Alexa Fluor 750	AF3548S-100UG
Alexa Fluor Plus 405	AF3548AFP405-100UG
Alexa Fluor Plus 488	AF3548AFP488-100UG
Alexa Fluor Plus 555	AF3548AFP555-100UG
Alexa Fluor Plus 594	AF3548AFP594-100UG
Alexa Fluor Plus 647	AF3548AFP647-100UG
Alexa Fluor Plus 680	AF3548AFP680-100UG
Allophycocyanin	AF3548A
Biotin	BAF3548
CoraFluor 1	AF3548CL1
DyLight 350	AF3548D
DyLight 405	AF3548E
DyLight 488	AF3548K
DyLight 550	AF3548L
DyLight 594	AF3548M
DyLight 650	AF3548W
DyLight 680	AF3548Y
DyLight 755	AF3548Z
FITC	AF3548F
HRP	AF3548H
Janelia Fluor 525	AF3548JF525
Janelia Fluor 549	AF3548I
Janelia Fluor 646	AF3548J
Janelia Fluor 669	AF3548JF669
PE	AF3548P
PerCP	AF3548C
mFluor Violet 450 SE	AF3548MFV450
mFluor Violet 500 SE	AF3548MFV500
mFluor Violet 610 SE	AF3548MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3548-SP

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MOSF20

Mouse Periostin/OSF-2 Quantikine ELISA Kit

(8 Publications)

1 Kit

MOSF20

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NBP2-45371

Periostin/OSF-2 Antibody (OTI2B2)

0.1 ml

NBP2-45371

Immunohistochemistry: Periostin/OSF-2 Antibody (OTI2B2) [NBP2-45371] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: Periostin/OSF-2 Antibody (OTI2B2) [NBP2-45371] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73336AF350
Alexa Fluor 405	NBP2-73336AF405
Alexa Fluor 488	NBP2-73336AF488
Alexa Fluor 532	NBP2-73336AF532
Alexa Fluor 594	NBP2-73336AF594
Alexa Fluor 647	NBP2-73336AF647
Alexa Fluor 700	NBP2-73336AF700
Alexa Fluor 750	NBP2-73336AF750
Alexa Fluor Plus 405	NBP2-73336AFP405
Alexa Fluor Plus 488	NBP2-73336AFP488
Alexa Fluor Plus 555	NBP2-73336AFP555
Alexa Fluor Plus 594	NBP2-73336AFP594
Alexa Fluor Plus 647	NBP2-73336AFP647
Alexa Fluor Plus 680	NBP2-73336AFP680
Allophycocyanin	NBP2-73336APC
Biotin	NBP2-73336B
CoraFluor 1	NBP2-73336CL1
DyLight 350	NBP2-73336UV
DyLight 405	NBP2-73336V
DyLight 488	NBP2-73336G
DyLight 550	NBP2-73336R
DyLight 594	NBP2-73336DL594
DyLight 650	NBP2-73336C
DyLight 680	NBP2-73336FR
DyLight 755	NBP2-73336IR
FITC	NBP2-73336F
HRP	NBP2-73336H
Janelia Fluor 525	NBP2-73336JF525
Janelia Fluor 549	NBP2-73336JF549
Janelia Fluor 646	NBP2-73336JF646
Janelia Fluor 669	NBP2-73336JF669
PE	NBP2-73336PE
PerCP	NBP2-73336PCP
mFluor Violet 450 SE	NBP2-73336MFV450
mFluor Violet 500 SE	NBP2-73336MFV500
mFluor Violet 610 SE	NBP2-73336MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73336

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NBP3-15128

Periostin/OSF-2 Antibody (CL12087) - BSA Free

100 ul

NBP3-15128

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Staining of human astrocytoma shows moderate positivity in tumor cells.

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Staining of human breast cancer shows strong positivity in extracellular matrix.

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Staining of human fallopian tube shows moderate to strong positivity in the extracellular matrix.

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: Periostin/OSF-2 Antibody (CL12087) [NBP3-15128] - Staining of human stomach shows strong positivity in extracellular matrix, as well as moderate positivity in glandular cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44358AF350
Alexa Fluor 405	NBP3-44358AF405
Alexa Fluor 488	NBP3-44358AF488
Alexa Fluor 532	NBP3-44358AF532
Alexa Fluor 594	NBP3-44358AF594
Alexa Fluor 647	NBP3-44358AF647
Alexa Fluor 700	NBP3-44358AF700
Alexa Fluor 750	NBP3-44358AF750
Alexa Fluor Plus 405	NBP3-44358AFP405
Alexa Fluor Plus 488	NBP3-44358AFP488
Alexa Fluor Plus 555	NBP3-44358AFP555
Alexa Fluor Plus 594	NBP3-44358AFP594
Alexa Fluor Plus 647	NBP3-44358AFP647
Alexa Fluor Plus 680	NBP3-44358AFP680
Allophycocyanin	NBP3-44358APC
Biotin	NBP3-44358B
CoraFluor 1	NBP3-44358CL1
DyLight 350	NBP3-44358UV
DyLight 405	NBP3-44358V
DyLight 488	NBP3-44358G
DyLight 550	NBP3-44358R
DyLight 594	NBP3-44358DL594
DyLight 650	NBP3-44358C
DyLight 680	NBP3-44358FR
DyLight 755	NBP3-44358IR
FITC	NBP3-44358F
HRP	NBP3-44358H
Janelia Fluor 525	NBP3-44358JF525
Janelia Fluor 549	NBP3-44358JF549
Janelia Fluor 646	NBP3-44358JF646
Janelia Fluor 669	NBP3-44358JF669
PE	NBP3-44358PE
PerCP	NBP3-44358PCP
mFluor Violet 450 SE	NBP3-44358MFV450
mFluor Violet 500 SE	NBP3-44358MFV500
mFluor Violet 610 SE	NBP3-44358MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-15128-25ul
Azide and BSA Free	NBP3-44358

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MAB2955

Periostin/OSF-2 Antibody (345616)

(3 Publications)

500 μg

MAB2955

2955-F2) was serially diluted and captured by Rat Anti-Mouse Periostin/OSF‑2 Monoclonal Antibody (Catalog # MAB2955) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Periostin/OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

2955-F2) was serially diluted and captured by Rat Anti-Mouse Periostin/OSF‑2 Monoclonal Antibody (Catalog # MAB2955) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Periostin/OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse Periostin/OSF‑2 (Catalog # 2955-F2) was serially diluted and captured by Rat Anti-Mouse Periostin/OSF‑2 Monoclonal Antibody (Catalog # MAB2955) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Periostin/OSF‑2 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2955) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2955U-100UG
Alexa Fluor 405	FAB2955V-100UG
Alexa Fluor 488	FAB2955G-100UG
Alexa Fluor 532	FAB2955X-100UG
Alexa Fluor 594	FAB2955T-100UG
Alexa Fluor 647	FAB2955R-100UG
Alexa Fluor 700	FAB2955N-100UG
Alexa Fluor 750	FAB2955S-100UG
Alexa Fluor Plus 405	FAB2955AFP405-100UG
Alexa Fluor Plus 488	FAB2955AFP488-100UG
Alexa Fluor Plus 555	FAB2955AFP555-100UG
Alexa Fluor Plus 594	FAB2955AFP594-100UG
Alexa Fluor Plus 647	FAB2955AFP647-100UG
Alexa Fluor Plus 680	FAB2955AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2955-SP

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DY2955

Mouse Periostin/OSF-2 DuoSet ELISA, 15 Plate

(2 Publications)

1 Kit (for 15 Plates)

DY2955

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Postn Products By Gene Name

Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free

Periostin/OSF-2 Antibody [Unconjugated]

Recombinant Human Periostin/OSF-2 Protein, CF

Periostin/OSF-2 Antibody - BSA Free

Recombinant Mouse Periostin/OSF-2 Protein, CF

Periostin/OSF-2 Antibody (345613) [Unconjugated]

Periostin/OSF-2 Antibody [Unconjugated]

Mouse Periostin/OSF-2 Quantikine ELISA Kit

Periostin/OSF-2 Antibody (OTI2B2)

Periostin/OSF-2 Antibody (CL12087) - BSA Free

Periostin/OSF-2 Antibody (345616)

Mouse Periostin/OSF-2 DuoSet ELISA, 15 Plate