MMP-9: Novus Biologicals

NBP2-59699

MMP-9 Antibody (S51-82) - BSA Free

(1 Publication)

100 μg

NBP2-59699

Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699] - Analysis using Mouse Anti-MMP9 Monoclonal Antibody, Clone S51-82 . Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) MMP9 Antibody (D) Composite.

MMP-9-Antibody-S51-82-Immunocytochemistry-Immunofluorescence-NBP2-59699-img0003.jpg

Identification of MET-like structures within human fetal membrane tissues infected with GBS ex vivo. Fetal membrane tissues were excised from healthy, term placental tissues from women undergoing routine cesarean section. (A and B) Fetal membrane tissues were then infected with GBS on the choriodecidual surface for 48 h prior to fixation and immunohistochemistry (A) or confocal microscopy (B). (A) Fetal membrane tissues were stained with hematoxylin and eosin (H & E), and representative images are shown at a magnification of ×4. The area within the red box is shown in sections stained with anti-GBS, anti-CD163, or anti-histone H3 antibodies and visualized by immunohistochemistry. GBS cells are able to invade from the choriodecidual surface (CD) toward the amnion epithelium (AE). Macrophages are shown in the area of GBS infection, and macrophages with extracellular histone staining (rightmost image, insert with ×40 magnification) are demonstrated in an area that is also stained with the macrophage marker CD163 (red boxes). Bars represent 100 μm. (B) Fixed and paraffin-embedded fetal membranes were stained with conjugated primary antibodies against CD163, histone H3, and MMP-9. CD163-positive cells within the membrane tissue are seen extruding contents that stain positive for histones and MMP-9 consistent with METs (white arrowheads). Bars represent 20 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30459195), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-59699AF350
Alexa Fluor 405	NBP2-59699AF405
Alexa Fluor 488	NBP2-59699AF488
Alexa Fluor 532	NBP2-59699AF532
Alexa Fluor 594	NBP2-59699AF594
Alexa Fluor 647	NBP2-59699AF647
Alexa Fluor 700	NBP2-59699AF700
Alexa Fluor 750	NBP2-59699AF750
Alexa Fluor Plus 405	NBP2-59699AFP405
Alexa Fluor Plus 488	NBP2-59699AFP488
Alexa Fluor Plus 555	NBP2-59699AFP555
Alexa Fluor Plus 594	NBP2-59699AFP594
Alexa Fluor Plus 647	NBP2-59699AFP647
Alexa Fluor Plus 680	NBP2-59699AFP680
Allophycocyanin	NBP2-59699APC
Biotin	NBP2-59699B
CoraFluor 1	NBP2-59699CL1
DyLight 350	NBP2-59699UV
DyLight 405	NBP2-59699V
DyLight 488	NBP2-59699G
DyLight 550	NBP2-59699R
DyLight 594	NBP2-59699DL594
DyLight 650	NBP2-59699C
DyLight 680	NBP2-59699FR
DyLight 755	NBP2-59699IR
FITC	NBP2-59699F
HRP	NBP2-59699H
Janelia Fluor 525	NBP2-59699JF525
Janelia Fluor 549	NBP2-59699JF549
Janelia Fluor 646	NBP2-59699JF646
Janelia Fluor 669	NBP2-59699JF669
PE	NBP2-59699PE
PerCP	NBP2-59699PCP
mFluor Violet 450 SE	NBP2-59699MFV450
mFluor Violet 500 SE	NBP2-59699MFV500
mFluor Violet 610 SE	NBP2-59699MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-59699-25ug

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DY6718

Mouse Total MMP-9 DuoSet ELISA, 15 Plate

(14 Publications)

1 Kit (for 15 Plates)

DY6718

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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AB911

MMP-9 Antibody [Unconjugated]

(6 Publications)

1 mg

AB911

Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

MMP‑9 was detected in immersion fixed MMP-9 transfected NS0 mouse myeloma cell line using Goat Anti-Human MMP‑9 Polyclonal Antibody (Catalog # AB911) at 5 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green). Specific labeling was localized to the cytoplasm. View our protocol for <a class=NoLineLink href=

Fluorescent ICC Staining of Cells on Coverslips." alt="MMP‑9 was detected in immersion fixed MMP-9 transfected NS0 mouse myeloma cell line using Goat Anti-Human MMP‑9 Polyclonal Antibody (Catalog # AB911) at 5 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green). Specific labeling was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-9 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human MMP-9 Polyclonal Antibody (Catalog # AB911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Neutrophil degranulation is increased in response to C3d treatment. Neutrophils isolated from HC individuals were incubated at 37 °C and remained unstimulated (Con) or were stimulated with C3d (2–40 µg/mL) or TNF-alpha (10 ng / 2 × 107 cells) (A–C). (A) Cell-free supernatants were collected at 10 min and immunoblotted for markers of tertiary granule (MMP-9), secondary granule (hCAP-18), or primary granule (BPI) release. Densitometry of MMP-9 (B), hCAP-18 (C), and BLPI (D) immunobands was performed. Western blot analyses of whole cell lysates demonstrated equal expression levels of beta -actin, confirming the use of equal cell numbers per reaction. C3d-challenged neutrophils released significantly greater levels of all three granule types when compared to untreated controls (Con) (n = 5 biological repeats, ANOVA followed by Bonferroni post-hoc test for selected groups). All results are expressed as relative densitometry units (DU), with representative Western blots presented. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34944741), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP2-41233

MMP-9 Antibody - BSA Free

(2 Publications)

0.1 mg

NBP2-41233

Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody - BSA Free [NBP2-41233] - Immunofluorescence of MMP-9 in human lung tissue with MMP-9 antibody at 20 ug/mL.

MLKL in macrophages affects myofibroblasts activities through PGE2. (A) The mRNA and protein expression of VEGF, MMP-9 in MLKL+/+ myofibroblasts treated with MLKL−/− M1ø CM or with PGE2 supplement. (B) The mRNA and protein expression of VEGF, MMP-9 in MLKL+/+ myofibroblasts treated with MLKL−/− M2ø CM or with PGE2 supplement. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean +/- SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test (n = 4–6). *P < 0.05, **P < 0.01, ***P < 0.001. ns not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40253554), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

NLRP3 is involved in tissue growth related factors expression in wound site (A-D) Skin wound tissues from wild-type and NLRP3−/− mice were subjected to immunofluorescence staining for MMP-9 (green), ER alpha (red), EGF (green), VEGF (red), and nuclei (DAPI, blue) on days 3, 5, and 7 post-injury. The merged images represent the composite pictures of the target protein and nuclei. Immunofluorescence staining was visualized using fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean fluorescence intensity was quantified (a.u., arbitrary units). The mRNA expression of MMP-9, ER alpha , EGF, and VEGF in wild-type and NLRP3−/− skin wound tissues was quantified using RT-PCR. GAPDH serves as internal reference. Results are expressed as mean +/- SD from multiple independent experiments and were analyzed using Student t test (n = 5). Statistical significance is indicated as follows: *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar = 20 μm Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39881348), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Rb

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-41233AF350
Alexa Fluor 405	NBP2-41233AF405
Alexa Fluor 488	NBP2-41233AF488
Alexa Fluor 532	NBP2-41233AF532
Alexa Fluor 594	NBP2-41233AF594
Alexa Fluor 647	NBP2-41233AF647
Alexa Fluor 700	NBP2-41233AF700
Alexa Fluor 750	NBP2-41233AF750
Alexa Fluor Plus 405	NBP2-41233AFP405
Alexa Fluor Plus 488	NBP2-41233AFP488
Alexa Fluor Plus 555	NBP2-41233AFP555
Alexa Fluor Plus 594	NBP2-41233AFP594
Alexa Fluor Plus 647	NBP2-41233AFP647
Alexa Fluor Plus 680	NBP2-41233AFP680
Allophycocyanin	NBP2-41233APC
Biotin	NBP2-41233B
CoraFluor 1	NBP2-41233CL1
DyLight 350	NBP2-41233UV
DyLight 405	NBP2-41233V
DyLight 488	NBP2-41233G
DyLight 550	NBP2-41233R
DyLight 594	NBP2-41233DL594
DyLight 650	NBP2-41233C
DyLight 680	NBP2-41233FR
DyLight 755	NBP2-41233IR
FITC	NBP2-41233F
HRP	NBP2-41233H
Janelia Fluor 525	NBP2-41233JF525
Janelia Fluor 549	NBP2-41233JF549
Janelia Fluor 646	NBP2-41233JF646
Janelia Fluor 669	NBP2-41233JF669
PE	NBP2-41233PE
PerCP	NBP2-41233PCP
mFluor Violet 450 SE	NBP2-41233MFV450
mFluor Violet 500 SE	NBP2-41233MFV500
mFluor Violet 610 SE	NBP2-41233MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-41233-0.025mg

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5427-MM

Recombinant Rat MMP-9 Protein, CF

(1 Review)

(4 Publications)

10 μg

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

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NBP2-22181

MMP-9 Antibody (5G3)

(1 Publication)

0.1 ml

NBP2-22181

Immunohistochemistry-Paraffin: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of tissues using MMP9 mouse mAb with DAB staining.

Flow Cytometry: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of Hela cells using MMP9 mouse mAb (blue) and negative control (red).

Western Blot: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis using MMP9 mAb against HEK293 (1), MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate.

ELISA: MMP-9 Antibody (5G3) [NBP2-22181] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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MAB9092

MMP-9 Antibody (116134) [Unconjugated] - Pro form

(5 Publications)

500 μg

MAB9092

909-MM) was serially diluted and captured by Rat Anti-Mouse Pro MMP‑9 Monoclonal Antibody (Catalog # MAB9092) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Pro MMP‑9 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox">

Recombinant Mouse MMP‑9 (Catalog # <a class=

909-MM) was serially diluted and captured by Rat Anti-Mouse Pro MMP‑9 Monoclonal Antibody (Catalog # MAB9092) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Pro MMP‑9 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Mouse MMP‑9 (Catalog # 909-MM) was serially diluted and captured by Rat Anti-Mouse Pro MMP‑9 Monoclonal Antibody (Catalog # MAB9092) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Pro MMP‑9 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB9092U-100UG
Alexa Fluor 405	FAB9092V-100UG
Alexa Fluor 488	FAB9092G-100UG
Alexa Fluor 532	FAB9092X-100UG
Alexa Fluor 594	FAB9092T-100UG
Alexa Fluor 647	FAB9092R-100UG
Alexa Fluor 700	FAB9092N-100UG
Alexa Fluor 750	FAB9092S-100UG
Alexa Fluor Plus 405	FAB9092AFP405-100UG
Alexa Fluor Plus 488	FAB9092AFP488-100UG
Alexa Fluor Plus 555	FAB9092AFP555-100UG
Alexa Fluor Plus 594	FAB9092AFP594-100UG
Alexa Fluor Plus 647	FAB9092AFP647-100UG
Alexa Fluor Plus 680	FAB9092AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB9092-SP

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NBP1-85575

MMP-9 Antibody - BSA Free

(2 Publications)

0.1 ml

NBP1-85575

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.

Staining of human kidney shows no positivity in cells in tubules as expected.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry analysis in human bone marrow and skeletal muscle tissues using HPA001238 antibody. Corresponding MMP9 RNA-seq data are presented for the same tissues.

Staining of human cell line U-2 OS shows localization to cytosol.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85575-25ul

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DY8174-05

Rat Total MMP-9 DuoSet ELISA, 5 Plate

(4 Publications)

1 Kit (for 5 Plates)

DY8174-05

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

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DY909

Mouse Pro-MMP-9 DuoSet ELISA, 15 Plate

(3 Publications)

1 Kit (for 15 Plates)

DY909

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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WBC018

Recombinant Human MMP-9 Western Blot Standard Protein

(3 Publications)

1 Vial

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-52948

MMP-9 Antibody (CL0538) - BSA Free

0.1 ml

NBP2-52948

Immunohistochemistry: MMP-9 Antibody (CL0538) [NBP2-52948] - Staining of human duodenum shows strong cytoplasmic positivity in a subset of lymphoid cells.

Immunohistochemistry-Paraffin: MMP-9 Antibody (CL0538) [NBP2-52948] - Staining of human lung shows strong positivity in lymphoid cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44015AF350
Alexa Fluor 405	NBP3-44015AF405
Alexa Fluor 488	NBP3-44015AF488
Alexa Fluor 532	NBP3-44015AF532
Alexa Fluor 594	NBP3-44015AF594
Alexa Fluor 647	NBP3-44015AF647
Alexa Fluor 700	NBP3-44015AF700
Alexa Fluor 750	NBP3-44015AF750
Alexa Fluor Plus 405	NBP3-44015AFP405
Alexa Fluor Plus 488	NBP3-44015AFP488
Alexa Fluor Plus 555	NBP3-44015AFP555
Alexa Fluor Plus 594	NBP3-44015AFP594
Alexa Fluor Plus 647	NBP3-44015AFP647
Alexa Fluor Plus 680	NBP3-44015AFP680
Allophycocyanin	NBP3-44015APC
Biotin	NBP3-44015B
CoraFluor 1	NBP3-44015CL1
DyLight 350	NBP3-44015UV
DyLight 405	NBP3-44015V
DyLight 488	NBP3-44015G
DyLight 550	NBP3-44015R
DyLight 594	NBP3-44015DL594
DyLight 650	NBP3-44015C
DyLight 680	NBP3-44015FR
DyLight 755	NBP3-44015IR
FITC	NBP3-44015F
HRP	NBP3-44015H
Janelia Fluor 525	NBP3-44015JF525
Janelia Fluor 549	NBP3-44015JF549
Janelia Fluor 646	NBP3-44015JF646
Janelia Fluor 669	NBP3-44015JF669
PE	NBP3-44015PE
PerCP	NBP3-44015PCP
mFluor Violet 450 SE	NBP3-44015MFV450
mFluor Violet 500 SE	NBP3-44015MFV500
mFluor Violet 610 SE	NBP3-44015MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44015
BSA Free	NBP2-52948-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Mmp9 Products By Gene Name

MMP-9 Antibody (S51-82) - BSA Free

Mouse Total MMP-9 DuoSet ELISA, 15 Plate

MMP-9 Antibody [Unconjugated]

MMP-9 Antibody - BSA Free

Recombinant Rat MMP-9 Protein, CF

MMP-9 Antibody (5G3)

MMP-9 Antibody (116134) [Unconjugated] - Pro form

MMP-9 Antibody - BSA Free

Rat Total MMP-9 DuoSet ELISA, 5 Plate

Mouse Pro-MMP-9 DuoSet ELISA, 15 Plate

Recombinant Human MMP-9 Western Blot Standard Protein

MMP-9 Antibody (CL0538) - BSA Free