Il3 Products By Gene Name

BT-003-GMP, BT-003-AFL, 203-IL; 50 ng/mL), SCF (Catalog #s BT-SCF-GMP, BT-SCF-AFL, BT-SCF; 100 ng/mL), TPO (Catalog #s BT-TPO-GMP, BT-TPO-AFL, BT-TPO; 100 ng/mL), FLT-3L (Catalog # BT-FT3L-GMP, BT-FT3L-AFL, BT-FT3L; 100 ng/mL), and SR1 (Catalog # 7086, 1 µM). After 7 days, frequency (%) of CD34+ cells was analyzed by flow cytometry with an anti-human CD34 monoclonal antibody (Catalog # MAB7227). CD34+ Cell Expansion is calculated by multiplying Total Nucleated Cell (TNC) fold expansion by the percent CD34+ cells. Cell viability on Day 7 was greater than 90% for all conditions." class="big_lightbox"> BT-003-GMP, BT-003-AFL, 203-IL; 50 ng/mL), SCF (Catalog #s BT-SCF-GMP, BT-SCF-AFL, BT-SCF; 100 ng/mL), TPO (Catalog #s BT-TPO-GMP, BT-TPO-AFL, BT-TPO; 100 ng/mL), FLT-3L (Catalog # BT-FT3L-GMP, BT-FT3L-AFL, BT-FT3L; 100 ng/mL), and SR1 (Catalog # 7086, 1 µM). After 7 days, frequency (%) of CD34+ cells was analyzed by flow cytometry with an anti-human CD34 monoclonal antibody (Catalog # MAB7227). CD34+ Cell Expansion is calculated by multiplying Total Nucleated Cell (TNC) fold expansion by the percent CD34+ cells. Cell viability on Day 7 was greater than 90% for all conditions." class="big_thumb" />

403-ML) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-403-NA). The ND₅₀ is typically 0.01-0.03 µg/mL." class="big_lightbox"> 403-ML) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-403-NA). The ND₅₀ is typically 0.01-0.03 µg/mL." alt="Recombinant Mouse IL‑3 (Catalog # 403-ML) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-403-NA). The ND₅₀ is typically 0.01-0.03 µg/mL." class="big_thumb" />

403-ML) was serially diluted and captured by Rat Anti-Mouse IL‑3 Monoclonal Antibody (Catalog # MAB403) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-403-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 403-ML) was serially diluted and captured by Rat Anti-Mouse IL‑3 Monoclonal Antibody (Catalog # MAB403) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-403-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL‑3 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) (positive staining) and HL‑60 human acute promyelocytic leukemia cell line (negative staining) using Goat Anti-Human IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-203-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-3 Antigen Affinity-purified Polyclonal Anti-body (Catalog # AF-203-NA). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_lightbox"> 203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-3 Antigen Affinity-purified Polyclonal Anti-body (Catalog # AF-203-NA). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_thumb" />

203-IL) was serially diluted and captured by Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-203-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 203-IL) was serially diluted and captured by Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-203-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

203-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB203). The ND₅₀ is typically 0.03-0.08 µg/mL." class="big_lightbox"> 203-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB203). The ND₅₀ is typically 0.03-0.08 µg/mL." alt="Recombinant Human IL‑3 (Catalog # 203-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB203). The ND₅₀ is typically 0.03-0.08 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Monoclonal Antibody (Catalog # MAB603). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_lightbox"> 203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Monoclonal Antibody (Catalog # MAB603). The ND₅₀ is typically 0.1-0.3 µg/mL." alt="Recombinant Human IL-3 (Catalog # 203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Monoclonal Antibody (Catalog # MAB603). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_thumb" />

203-IL) was serially diluted and captured by Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-203-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 203-IL) was serially diluted and captured by Mouse Anti-Human IL‑3 Monoclonal Antibody (Catalog # MAB603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-203-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-3 was detected in immersion fixed rat splenocytes using Goat Anti-Rat IL-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2524) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

2524-RL) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2524). The ND₅₀ is typically 0.75-3 µg/mL." class="big_lightbox"> 2524-RL) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2524). The ND₅₀ is typically 0.75-3 µg/mL." class="big_thumb" />

203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-3 Polyclonal Antibody (Catalog # AB-203-NA). The ND₅₀ is typically 0.4-2 µg/mL." class="big_lightbox"> 203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-3 Polyclonal Antibody (Catalog # AB-203-NA). The ND₅₀ is typically 0.4-2 µg/mL." alt="Recombinant Human IL-3 (Catalog # 203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-3 Polyclonal Antibody (Catalog # AB-203-NA). The ND₅₀ is typically 0.4-2 µg/mL." class="big_thumb" />