Il12b Products By Gene Name

839-B1) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human IL-12/IL-23 p40 Subunit (Catalog # 309-IL) binds with an ED₅₀ of 1-6 ng/mL." class="big_lightbox"> 839-B1) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human IL-12/IL-23 p40 Subunit (Catalog # 309-IL) binds with an ED₅₀ of 1-6 ng/mL." alt="When Recombinant Human IL-12 R beta 1 Fc Chimera (Catalog # 839-B1) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human IL-12/IL-23 p40 Subunit (Catalog # 309-IL) binds with an ED₅₀ of 1-6 ng/mL." class="big_thumb" />

1954-FL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1954). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_lightbox"> 1954-FL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1954). The ND₅₀ is typically 0.5-2.0 µg/mL." alt="Recombinant Feline IL-12 (Catalog # 1954-FL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1954). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF019). A specific band was detected for IL-12/IL-23 p40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF019). A specific band was detected for IL-12/IL-23 p40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of Hs 294T human melanoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL-12/IL-23 p40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

1290-IL) induces IL-17 secretion in the mouse splenocytes in a dose-dependent manner (orange line), as measured by Mouse IL-17 Quantikine kit. IL-17 Secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-12/IL-23 p40 Anti-gen Affinity-purified Polyclonal Antibody (Catalog # AF309). The ND₅₀ is typically 0.004-0.02 µg/mL." class="big_lightbox"> 1290-IL) induces IL-17 secretion in the mouse splenocytes in a dose-dependent manner (orange line), as measured by Mouse IL-17 Quantikine kit. IL-17 Secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-12/IL-23 p40 Anti-gen Affinity-purified Polyclonal Antibody (Catalog # AF309). The ND₅₀ is typically 0.004-0.02 µg/mL." class="big_thumb" />

499-ML) was serially diluted and captured by Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB499) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑12/IL‑23 p40 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 499-ML) was serially diluted and captured by Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB499) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑12/IL‑23 p40 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Mouse IL‑12/IL‑23 p40 Homodimer (Catalog # 499-ML) was serially diluted and captured by Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB499) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑12/IL‑23 p40 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

309-IL) was serially diluted and captured by Mouse Anti-Human IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB609) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 309-IL) was serially diluted and captured by Mouse Anti-Human IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB609) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human IL‑12/IL‑23 p40 Monomer (Catalog # 309-IL) was serially diluted and captured by Mouse Anti-Human IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB609) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2118-CL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Canine IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1969). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_lightbox"> 2118-CL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Canine IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1969). The ND₅₀ is typically 0.1-0.4 µg/mL." alt="Recombinant Canine IL-12 (2118-CL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Canine IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1969). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_thumb" />