| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-75266-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11737-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11737 | |||
| Azide and BSA Free | NBP2-11737-0.025mg | |||
![Western Blot: Integrin alpha 3/CD49c Antibody (A7H2) [NBP3-32475] - Western blot analysis of Integrin alpha 3/CD49c on different lysates with Mouse anti-Integrin alpha 3/CD49c antibody (NBP3-32475) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 ug/Lane) Lane 2: HUVEC cell lysate (10 ug/Lane) Lane 3: A431 cell lysate (10 ug/Lane) Lane 4: Human kidney tissue lysate (20 ug/Lane) Predicted band size: 117 kDa Observed band size: 130 kDa Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32475) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32475_mouse-integrin-alpha-3-cd49c-mab-a7h2-278202415212430.jpg "Western Blot: Integrin alpha 3/CD49c Antibody (A7H2) [NBP3-32475] - Western blot analysis of Integrin alpha 3/CD49c on different lysates with Mouse anti-Integrin alpha 3/CD49c antibody (NBP3-32475) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 ug/Lane) Lane 2: HUVEC cell lysate (10 ug/Lane) Lane 3: A431 cell lysate (10 ug/Lane) Lane 4: Human kidney tissue lysate (20 ug/Lane) Predicted band size: 117 kDa Observed band size: 130 kDa Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32475) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Integrin alpha 3/CD49c Antibody (A7H2) [NBP3-32475] - Immunocytochemistry analysis of U-87 MG cells labeling Integrin alpha 3/CD49c with Mouse anti-Integrin alpha 3/CD49c/CD49c antibody (NBP3-32475) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Integrin alpha 3/CD49c antibody (NBP3-32475) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32475_mouse-integrin-alpha-3-cd49c-mab-a7h2-2920241114845.jpg)
![Western Blot: Integrin alpha 3 Overexpression Lysate (Adult Normal) [NBL1-12063] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Integrin alpha 3.](http://images.novusbio.com/fullsize/Integrin-alpha-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12063-img0002.jpg "Western Blot: Integrin alpha 3 Overexpression Lysate (Adult Normal) [NBL1-12063] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Integrin alpha 3.")
![Western Blot: Integrin alpha 3 Overexpression Lysate (Adult Normal) [NBP2-10464] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Integrin alpha 3.](http://images.novusbio.com/fullsize/Integrin-alpha-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10464-img0001.jpg "Western Blot: Integrin alpha 3 Overexpression Lysate (Adult Normal) [NBP2-10464] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Integrin alpha 3.")