IFNG Products By Gene Name

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IFN-gamma was detected in immersion fixed rat splenocytes treated with PMA and calcium ionomycin using Mouse Anti-Rat IFN-gamma Monoclonal Antibody (Catalog # MAB5851) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

585-IF) was serially diluted and captured by Mouse Anti-Rat IFN‑ gamma Monoclonal Antibody (Catalog # MAB5851) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 585-IF) was serially diluted and captured by Mouse Anti-Rat IFN‑ gamma Monoclonal Antibody (Catalog # MAB5851) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-485-NA). The ND₅₀ is typically 0.2-1.0 µg/mL." class="big_lightbox"> 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-485-NA). The ND₅₀ is typically 0.2-1.0 µg/mL." alt="Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-485-NA). The ND₅₀ is typically 0.2-1.0 µg/mL." class="big_thumb" />

485-MI) was serially diluted and captured by Rat Anti-Mouse IFN‑ gamma Monoclonal Antibody (Catalog # MAB785) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-485-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 485-MI) was serially diluted and captured by Rat Anti-Mouse IFN‑ gamma Monoclonal Antibody (Catalog # MAB785) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-485-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

781-CG) reduces the Vesticular Stomatitis Virus (VSV)-induced cytopathy in the A-72 canine fibroma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of VSV activity elicited by Recombinant Canine IFN-gamma (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF781). The ND₅₀ is typically 0.2-1 µg/mL." class="big_lightbox"> 781-CG) reduces the Vesticular Stomatitis Virus (VSV)-induced cytopathy in the A-72 canine fibroma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of VSV activity elicited by Recombinant Canine IFN-gamma (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF781). The ND₅₀ is typically 0.2-1 µg/mL." alt="Recombinant Canine IFN-gamma (Catalog # 781-CG) reduces the Vesticular Stomatitis Virus (VSV)-induced cytopathy in the A-72 canine fibroma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of VSV activity elicited by Recombinant Canine IFN-gamma (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF781). The ND₅₀ is typically 0.2-1 µg/mL." class="big_thumb" />

1586-HG) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Equine IFN-gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586). The ND₅₀ is typically 0.25-1.25 µg/mL." class="big_lightbox"> 1586-HG) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Equine IFN-gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586). The ND₅₀ is typically 0.25-1.25 µg/mL." alt="Recombinant Equine IFN-gamma (Catalog # 1586-HG) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Equine IFN-gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586). The ND₅₀ is typically 0.25-1.25 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1586-HG) was serially diluted and captured by Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1586-HG) was serially diluted and captured by Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1586-HG) was serially diluted and captured by Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1586-HG) was serially diluted and captured by Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="IFN-gamma was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Feline IFN-gamma Monoclonal Antibody (Catalog # MAB764) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

961-RM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rhesus Macaque IFN-gamma (200 ng/mL) is neutralized (green line) by increasing concentrations of Rhesus Macaque IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961). The ND₅₀ is typically 1.5-5.0 µg/mL." class="big_lightbox"> 961-RM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rhesus Macaque IFN-gamma (200 ng/mL) is neutralized (green line) by increasing concentrations of Rhesus Macaque IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961). The ND₅₀ is typically 1.5-5.0 µg/mL." alt="Recombinant Rhesus Macaque IFN-gamma (Catalog # 961-RM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rhesus Macaque IFN-gamma (200 ng/mL) is neutralized (green line) by increasing concentrations of Rhesus Macaque IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961). The ND₅₀ is typically 1.5-5.0 µg/mL." class="big_thumb" />

961-RM) was serially diluted and captured by Goat Anti-Rhesus Macaque IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rhesus Macaque IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 961-RM) was serially diluted and captured by Goat Anti-Rhesus Macaque IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rhesus Macaque IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

961-RM) was serially diluted and captured by Goat Anti-Rhesus Macaque IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rhesus Macaque IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 961-RM) was serially diluted and captured by Goat Anti-Rhesus Macaque IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rhesus Macaque IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF961) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

585-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Rat IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat IFN-gamma Monoclonal Antibody (Catalog # MAB585). The ND₅₀ is typically 0.5-1.5 µg/mL." class="big_lightbox"> 585-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Rat IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat IFN-gamma Monoclonal Antibody (Catalog # MAB585). The ND₅₀ is typically 0.5-1.5 µg/mL." alt="Recombinant Rat IFN-gamma (Catalog # 585-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Rat IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat IFN-gamma Monoclonal Antibody (Catalog # MAB585). The ND₅₀ is typically 0.5-1.5 µg/mL." class="big_thumb" />

485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB485R). The ND₅₀ is typically 0.075-0.3 µg/mL." class="big_lightbox"> 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB485R). The ND₅₀ is typically 0.075-0.3 µg/mL." alt="Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB485R). The ND₅₀ is typically 0.075-0.3 µg/mL." class="big_thumb" />

MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />