| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97872-0.2mg | |||
| BSA Free | NBP1-97872-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89951-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AP-100-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59339-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61122-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46806-25ul | |||
![Western Blot: Hsp70 / Hsc70 Antibody [NBP1-97872] - Analysis of HSC70/HSP70, pAb Lane 1: MW Marker , Lane 2: 3T3 Cell Lysate Lane 3: 3T3 Cell Lysate (heat shocked) Lane 4: PC-12 Cell Lysate , Lane 5: PC-12 Cell Lysate (heat shocked) Lane 6: HeLa Cell Lysate Lane 7: HeLa Cell Lysate (heat shocked), Lane 8: HSP70/HSP72 (human), (recombinant) Lane 9: HSC70/HSP73 (bovine), (recombinant).](http://images.novusbio.com/fullsize/Hsp70---Hsc70-Antibody-Western-Blot-NBP1-97872-img0003.jpg "Western Blot: Hsp70 / Hsc70 Antibody [NBP1-97872] - Analysis of HSC70/HSP70, pAb Lane 1: MW Marker , Lane 2: 3T3 Cell Lysate Lane 3: 3T3 Cell Lysate (heat shocked) Lane 4: PC-12 Cell Lysate , Lane 5: PC-12 Cell Lysate (heat shocked) Lane 6: HeLa Cell Lysate Lane 7: HeLa Cell Lysate (heat shocked), Lane 8: HSP70/HSP72 (human), (recombinant) Lane 9: HSC70/HSP73 (bovine), (recombinant).")
![Immunohistochemistry-Paraffin: HSP70 / HSC70 Antibody [NBP1-97872] - Analysis of human spleen, neutrophils tissue stained with HSC70/HSP70, pAb at 10ug/ml.](http://images.novusbio.com/fullsize/HSP70---HSC70-Antibody-Immunohistochemistry-Paraffin-NBP1-97872-img0001.jpg)
![Western Blot: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A: A549 Whole Cell LysateLane B: HCT116 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:70 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Western-Blot-NBP2-89951-img0005.jpg "Western Blot: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A: A549 Whole Cell LysateLane B: HCT116 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:70 kDaObserved band size:70 kDa(We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of HSPA1A in cynomolgus macaque testis with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89951-img0003.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Analysis of Human HSPA1A expression on HeLa cells. The cells were treated, stained with purified anti-Human HSPA1A, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Flow-Cytometry-NBP2-89951-img0004.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of HSPA1A in human hepatoma with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunohistochemistry-Paraffin-NBP2-89951-img0002.jpg)
![Immunoprecipitation: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Lane A:0.5 mg Hela Whole Cell Lysate. 2 uL anti-HSPA1A rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HSPA1A rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-001-Immunoprecipitation-NBP2-89951-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (001) [NBP2-89951] - Staining of Human HSPA1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89951 antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89951_rabbit-monoclonal-hsp70-hspa1a-antibody-001-235202521525031.png)
![Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Western-Blot-NBP2-59339-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis on non-permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate showing cell membrane staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: None. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin Texas red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Immunocytochemistry-Immunofluorescence-NBP2-59339-img0006.jpg)
![Flow (Cell Surface): HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - A cell surface stain was performed on HeLa cells with Hsp70 / Hsc70 Antibody (1H11) NBP2-59339PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cell-Surface-NBP2-59339-img0007.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Hsp70 / Hsc70 Antibody (1H11) [NBP2-59339] - Clone 1H11: FITC conjugate. Tissue: Jurkat E6.1. Species: Human. Unfixed/non-permeablized cells were incubated with (A) Anti-HSP70 control antibody (not cell-surface-specific), or (B) Anti-HSP70 antibody, clone 1H11 for 40 min at 4 degrees C. Fixed/permeabilized cells were incubated with (C) Anti-HSP70 control antibody, or (D) Anti-HSP70 antibody, clone 1H11 or 40 min at 4 degrees C. Analysis shows that non-permeabilized cells show cell surface binding with 1H11 clone (NBP2-59339) but not with the control antibody, and the permeabilized cells show strong intracellular binding with the control antibody but much less with 1H11 clone (NBP2-59339). Courtesy of: Dr. Elyse Ireland, Institute of Medicine, University of Chester.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-1H11-Flow-Cytometry-NBP2-59339-img0002.jpg)
![Western Blot: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70/HSPA1A protein using Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[ac-Lys77]-Antibody-Western-Blot-NBP2-61122-img0004.jpg "Western Blot: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70/HSPA1A protein using Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70/HSPA1A Polyclonal Antibody (NBP2-61122) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 uM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[ac-Lys77]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61122-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP70/HSPA1A [ac Lys77] Antibody [NBP2-61122] - Tissue: Colon Cancer. Species: Human. Primary Antibody: Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody (NBP2-61122) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-[ac-Lys77]-Antibody-Immunohistochemistry-Paraffin-NBP2-61122-img0003.jpg)
![Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Western blot analysis of extracts of various cell lines, using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5U9A0-Western-Blot-NBP3-16482-img0003.jpg "Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Western blot analysis of extracts of various cell lines, using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Immunofluorescence analysis of NIH-3T3 cells using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5U9A0-Immunocytochemistry-Immunofluorescence-NBP3-16482-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Immunofluorescence analysis of HeLa cells using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-5U9A0-Immunocytochemistry-Immunofluorescence-NBP3-16482-img0001.jpg)
![Western Blot: HSP70/HSPA1A Antibody (S01-6H6) [NBP3-19657] - Western blot detection of HSP70/HSPA1A in Hela cell lysates using NBP3-19657 (1:1000 diluted). Predicted band size: 70kDa. Observed band size: 70kDa.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-S01-6H6-Western-Blot-NBP3-19657-img0002.jpg "Western Blot: HSP70/HSPA1A Antibody (S01-6H6) [NBP3-19657] - Western blot detection of HSP70/HSPA1A in Hela cell lysates using NBP3-19657 (1:1000 diluted). Predicted band size: 70kDa. Observed band size: 70kDa.")
![Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (S01-6H6) [NBP3-19657] - Immunohistochemistry of HSP70/HSPA1A in paraffin-embedded Human tonsil using NBP3-19657 at dilution 1/50](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-S01-6H6-Immunohistochemistry-Paraffin-NBP3-19657-img0001.jpg)
![Western Blot: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445] - Western blot analysis of HSP70/HSPA1A on different lysates with Rabbit anti-HSP70/HSPA1A antibody (NBP3-32445) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: A431 cell lysate Lane 5: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32445) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32445_rabbit-hsp70-hspa1a-mab-pd01-65-278202416331233.jpg "Western Blot: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445] - Western blot analysis of HSP70/HSPA1A on different lysates with Rabbit anti-HSP70/HSPA1A antibody (NBP3-32445) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: A431 cell lysate Lane 5: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32445) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HSP70/HSPA1A antibody (NBP3-32445) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32445) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32445_rabbit-hsp70-hspa1a-mab-pd01-65-298202413585585.jpg)
![Recombinant Human HSP70/HSPA1A Protein [NBC1-18367]](http://images.novusbio.com/fullsize/Recombinant-Human-HSP70-HSPA1A-Protein-NBC1-18367-img0001.jpg "Recombinant Human HSP70/HSPA1A Protein [NBC1-18367]")