Ccl2 Products By Gene Name

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 25 µg/mL Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB2791) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

479-JE) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA). The ND₅₀ is typically 10-30 µg/mL." class="big_lightbox"> 479-JE) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse CCL2/JE/MCP-1 Polyclonal Antibody (Catalog # AB-479-NA). The ND₅₀ is typically 10-30 µg/mL." class="big_thumb" />

1774-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CCL2/JE/MCP-1 (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB28171). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_lightbox"> 1774-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CCL2/JE/MCP-1 (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB28171). The ND₅₀ is typically 0.5-2.0 µg/mL." alt="Recombinant Canine CCL2/JE/MCP-1 (Catalog # 1774-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CCL2/JE/MCP-1 (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB28171). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1774-MC) was serially diluted and captured by Mouse Anti-Canine CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB28171) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1774) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1774-MC) was serially diluted and captured by Mouse Anti-Canine CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB28171) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1774) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1774-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CCL2/JE/ MCP-1 (300 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine CCL2/JE/ MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1774). The ND₅₀ is typically 0.8-4.0 µg/mL." class="big_lightbox"> 1774-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CCL2/JE/ MCP-1 (300 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine CCL2/JE/ MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1774). The ND₅₀ is typically 0.8-4.0 µg/mL." alt="Recombinant Canine CCL2/JE/ MCP-1 (Catalog # 1774-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CCL2/JE/ MCP-1 (300 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine CCL2/JE/ MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1774). The ND₅₀ is typically 0.8-4.0 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1774-MC) was serially diluted and captured by Mouse Anti-Canine CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB28171) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1774) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1774-MC) was serially diluted and captured by Mouse Anti-Canine CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB28171) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine CCL2/JE/MCP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1774) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

279-MC) chemoattracts BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/ MCP-1 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL2/MCP-1 Polyclonal Antibody (Catalog # AB-279-NA). The ND₅₀ is typically 50-100 µg/mL." class="big_lightbox"> 279-MC) chemoattracts BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/ MCP-1 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL2/MCP-1 Polyclonal Antibody (Catalog # AB-279-NA). The ND₅₀ is typically 50-100 µg/mL." alt="Recombinant Human CCL2/ MCP-1 (Catalog # 279-MC) chemoattracts BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/ MCP-1 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL2/MCP-1 Polyclonal Antibody (Catalog # AB-279-NA). The ND₅₀ is typically 50-100 µg/mL." class="big_thumb" />

279-MC) was serially diluted 2-fold and captured by Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB679R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL2/JE/MCP-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF279) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> 279-MC) was serially diluted 2-fold and captured by Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB679R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL2/JE/MCP-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF279) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC) was serially diluted 2-fold and captured by Mouse Anti-Human CCL2/JE/MCP-1 Monoclonal Antibody (Catalog # MAB679R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL2/JE/MCP-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF279) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (75 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP-1 Monoclonal Antibody (Catalog # MAB679R). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_lightbox"> 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (75 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL2/MCP-1 Monoclonal Antibody (Catalog # MAB679R). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_thumb" />