360-MP), Recombinant Mouse CCL20/MIP-3a (Catalog #
760-M3) and Recombinant Rat CCL20/MIP-3a (Catalog #
540-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
HAF007). A specific band was detected for CCL20/MIP-3a at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_lightbox">

360-MP), Recombinant Mouse CCL20/MIP-3a (Catalog #
760-M3) and Recombinant Rat CCL20/MIP-3a (Catalog #
540-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
HAF007). A specific band was detected for CCL20/MIP-3a at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." alt="Western blot shows 100 ng of Recombinant Human CCL20/MIP-3a (Catalog #
360-MP), Recombinant Mouse CCL20/MIP-3a (Catalog #
760-M3) and Recombinant Rat CCL20/MIP-3a (Catalog #
540-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
HAF007). A specific band was detected for CCL20/MIP-3a at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_thumb" />
360-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog #
AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP-3a (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB360). The ND
50 is typically 0.2-2 µg/mL." class="big_lightbox">

360-MP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog #
AR002). Chemotaxis elicited by Recombinant Human CCL20/MIP-3a (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB360). The ND
50 is typically 0.2-2 µg/mL." class="big_thumb" />