CXCL8/IL-8: Novus Biologicals

DY208

Human IL-8/CXCL8 DuoSet ELISA, 15 Plate

(520 Publications)

1 Kit (for 5 Plates)

DY208

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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D8000C

Human IL-8/CXCL8 Quantikine ELISA Kit

(466 Publications)

1 Kit

D8000C

RT-qPCR for a set of cytokines (CSF2, IL6, IL8 and IL1B) and chemokines (CXCL1 and CXCL6) of total RNA samples derived from mono- and co-cultures in a transwell assay.The respective co- (+) or mono- (-) culture is indicated on the X-axis. The respective analyzed cytokine or chemokine is indicated in the header of each graph. Expression values are shown in arbitrary units (AU) and have been normalized to beta-2 microglobulin (B2M) mRNA copies. Experiments were performed in triplicates (n = 3). Statistical analysis was performed on the mean values by unpaired comparison of mono-cultured HDF and co-cultured HDF RNA samples by using Student’s t-test (**p<0.01, ***p<0.001; n.s.: not significant). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25919140), licensed under a CC-BY license. Not internally tested by R&D Systems.

Pathogen-associated molecular patterns (PAMPs) and damage-associated molecular pattern (DAMPs) induce cytokine expression in human breast cancer cells in vitro. a Relative expression of IL-6 and IL-8 mRNAs after 6 h stimulation with lipopolysaccharide 1 (LPS1) and LPS2, using quantitative real-time PCR (QPCR) on mRNA from the cell lines indicated; n = 6. Error bars standard error of the mean (SEM); ***P <0.001 (analysis of variance (ANOVA)). b Release of IL-6 and IL-8 after stimulation with LPS1 and cycloheximide for 6 h, using ELISA on supernatants from stimulated MDA-MB-231 cells; n = 3. c-e Relative release of IL-6 and IL-8 after stimulation with LPS1, LPS2, the DAMP HMGB1 or IL-1 beta for 6 h, using ELISA on supernatants from stimulated MDA-MB-231 cells (c), SUM-149 cells (d) and SUM-159 cells (e); n = 10. Error bars SEM; *P <0.05, ***P <0.001 (ANOVA). f Dual luciferase reporter assays of MDA-MB-231 cells transfected with an NF kappa B reporter. TK-Renilla was co-transfected as control (Ctrl). LPS1, LPS2, HMGB1 or IL-1 beta was added to stimulate NF kappa B activity as described in “Methods”; n = 14. Error bars SEM; ***P <0.001 (ANOVA). g The relative release of IL-6 and IL-8 after stimulation with the DAMP S100A9 for 20 h, using ELISA on supernatants from stimulated MDA-MB-231, SUM-149 and SUM-159 cells; n = 6. Error bars SEM. *P <0.05, ***P <0.001 (ANOVA). h Transient transfection of pDUO-MD2/hTLR4 for 72 h in MCF-7 cells induces release of both IL-6 and IL-8 measured by ELISA; n = 6. Error bars SEM; *P <0.05, ***P <0.001 (Students t test) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26392082), licensed under a CC-BY license. Not internally tested by R&D Systems.

Toll-like receptor 4 (TLR4) silencing decreases endogenous levels of pro-inflammatory cytokines. a Effect of TLR2/4 silencing in breast cancer cells transfected with negative control (nc) siRNA, or siRNA directed against TLR2 mRNA (si#1 and si#2) or TLR4 mRNA (si#1 and si#2) was analyzed using quantitative real-time PCR; ***P <0.001 (analysis of variance (ANOVA)). b IL-6 (left) and IL-8 (right) ELISA on supernatants from MDA-MB-231 breast cancer cells transfected with nc siRNA, or siRNA directed against TLR2 mRNA (si#1 and si#2) or TLR4 mRNA (si#1 and si#2); n = 4. Error bars standard error of the mean (SEM); *P <0.05, **P <0.01, ***P <0.001 (ANOVA). c Boyden chamber migration assays. Migration of primary human myeloid cells towards supernatants from different cell lines indicated. Human primary peripheral blood mononuclear cells were isolated as previously described [48] and allowed to migrate through a Costar Transwell® Permeable Support 8.0-μm 24-well plate (Corning) to the supernatants of breast cancer supernatants cultured under serum-free conditions. Percentage of migrated CD11b+ cells was analyzed using a flow cytometer and CD11b-APC antibodies (BD Sciences); n = 4. Error bars SEM; *P <0.05, **P <0.01, ***P <0.001 (ANOVA). d Matrigel invasion assays. Invasion of lipopolysaccharide (LPS)-stimulated/un-stimulated MDA-MB-231 cells into matrigel invasion chambers (BD Sciences) as indicated: 25 × 103 MDA-MB-231 cells were stimulated or not with LPS and allowed to invade from 72 h. Amount of invaded cells was analyzed using crystal violet staining and manual counting in four separate experiments; n = 4. Error bars SEM; *P <0.05, **P <0.01, ***P <0.001 (Student’s t test) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26392082), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	PD8000C
HRP	S8000C

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MAB208

CXCL8/IL-8 Antibody (6217) [Unconjugated]

(1 Review)

(204 Publications)

100 μg

MAB208

HAF018). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 3 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 3 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

<b>Biological Strategies Validation. </b>IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

<b>Biological Strategies Validation. </b>Simple Western lane view shows human PBMC conditioned media untreated (-) or treated (+) with 1 μg/mL LPS for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) . This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). The ND₅₀ is typically 0.08-0.4 µg/mL." class="big_lightbox">

Recombinant Human IL-8/CXCL8 (Catalog # <a class=

208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). The ND₅₀ is typically 0.08-0.4 µg/mL." class="big_thumb" />

CDK1/CDC14B-dependent phosphorylation of USP9X at serine 2563 promotes mitotic survival via WT1 and IL-8. e Immunoblot analysis confirming reversal of mitotic apoptosis following exogenous reconstitution of IL-8 in CXCL8-depleted cells. Experiment was performed as in a, with addition of exogenous IL-8 for the last 48 h. Cells were treated with nocodazole for 8 h. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32152317), licensed under a CC-BY license. Not internally tested by R&D Systems.

CDK1/CDC14B-dependent phosphorylation of USP9X at serine 2563 promotes mitotic survival via WT1 and IL-8. f Immunoblot analysis detecting induction of mitotic apoptosis in response to WT1, CXCL8, or WT1 and CXCL8 knockdown compared to control knockdown in U2OS cells that were arrested in mitosis using nocodazole (8 h). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32152317), licensed under a CC-BY license. Not internally tested by R&D Systems.

CDK1/CDC14B-dependent phosphorylation of USP9X at serine 2563 promotes mitotic survival via WT1 and IL-8.a. i Immunoblot analysis revealing decreased mitotic apoptosis after CDC14B knockdown in USP9XWT but not in USP9XMut U2OS cells. Before lysis cells were transfected with control or CDC14B-directed siRNA, arrested in mitosis, and collected for analysis by western blot. Throughout this figure, mean and standard deviations as error bars are displayed. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32152317), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cytokines regulated by RanBP1 in lung cancer. (A) Identification of secreted factors regulated by RanBP1 using cytokine arrays. A549 cells are used, and the expression of RanBP1 is suppressed using si-RNA. (B) Comparison of the gene expression of cytokines regulated by RanBP1. (C) Analysis of cytokines regulated by RanBP1 using WB. (D) Comparison of the sphere formation ability after each neutralizing antibody treatment. The p value is less than 0.0001 versus the IgG Ab value. (E) Comparison of migration and invasion ability of cells after each neutralizing antibody treatment. Experiments performed in triplicate. (F) CSC marker protein analysis in A549 after treatment with IL-18 neutralizing antibody. (G) Confirmation of the expression levels of EMT marker proteins after treatment with the IL-18 neutralizing antibody. (H) Expression analysis of RanBP1 regulated by IL-18 using neutralizing antibodies. Error bars represent mean ± SD. Triplicate samples. * p < 0.05, ** p < 0.001, versus control. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37047826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Interaction between osteosarcoma and macrophages increased IL-8 production. A Cytokine arrays of 143B-Luc cells, human macrophages, and co-culture CM. B IL-8 production during the co-culture of OS cells (143B-Luc, SJSA-1) and macrophages (THP-1 Mφ, HMDMs). C IL-8 expression in macrophages stimulated with OS-CM. D IL-8 expression in OS cells stimulated with macrophage CM. E UMAP plot of OS lung metastases. F UMAP plot of Iba1, CD163, and IL-8 expression in myeloid cell clusters. G Violin plot depicting IL-8 expression in Iba1+/− or CD163+/− myeloid cell clusters. CM conditioned medium, HMDMs human monocyte-derived macrophages, OS osteosarcoma, THP-1 Mφ THP-1-derived macrophage. Data are presented as mean ± standard deviation. **p < 0.01. All data were obtained from at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of IL-8 in co-culture CM on proliferation, migration, and invasion of osteosarcoma cells.A Cell viability assay for OS cells treated with or without recombinant IL-8 (rIL-8, 10 ng/mL) using Cell Counting Kit-8. B, C Scratch and invasion assays for OS cells treated with or without co-culture CM. The scratch assay was performed for 12 h, and the invasion assay was conducted for 24 h. Scale bars represent 500 μm. D Cell viability assay for OS cells treated with or without co-culture CM and with or without anti-IL-8 antibodies (IL-8 Abs, 1 µg/mL) using Cell Counting Kit-8. E, F Scratch and invasion assays for OS cells treated with or without co-culture CM and with or without IL-8 Abs (100 ng/mL). The scratch assay was performed for 12 h, and the invasion assay was conducted for 24 h. Scale bars represent 500 μm. CM: conditioned medium, OS: osteosarcoma, ns: not significant. Data are presented as mean ± standard deviation. *p < 0.05 and **p < 0.01. All data were obtained from at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of IL-8 in co-culture CM on lung metastasis. A Schematic showing the experimental design for OS tail vein injection. 143B-Luc cells were treated with DMEM or co-culture CM for 12 h prior to tail vein injection; 143B-Luc cells (1 × 106/mouse) were injected into the tail vein of 6–7 week-old BALB/c nu/nu mice. B IVIS imaging and quantification of lung metastasis 14 days after the injection of tumors pretreated with or without co-culture CM. C Hematoxylin and eosin staining of lung sections with or without co-culture CM and quantification of lung colonies. Scale bars represent 1 mm and 500 µm. D Immunohistochemistry of Ki-67 positive cells in lung colonies with or without co-culture CM and quantification of the Ki-67 labeling index. Two colonies per sample were randomly selected, and quantification was performed for 10 colonies. Ki-67-positive cells in the colonies were counted and shown as the Ki-67 labeling index. Scale bars represent 100 µm. E Schematic showing the experimental design for OS tail vein injection using anti-IL-8 antibody. F IVIS imaging and quantification of lung metastasis 14 days after the injection of tumors pretreated with co-culture CM with or without IL-8 antibodies. G Hematoxylin and eosin staining of lung sections with co-culture CM with or without IL-8 Abs and quantification of lung colonies. Scale bars represent 1 mm and 500 µm. H Immunohistochemistry of Ki-67-positive cells in lung colonies with co-culture CM with or without IL-8 Abs and quantification of Ki-67 labeling index. Scale bars represent 100 µm. CM conditioned medium, DMEM Dulbecco’s modified Eagle’s medium, IVIS, in vivo imaging system, OS osteosarcoma. Data are presented as mean ± standard deviation; *p < 0.05, **p < 0.01. Each group contained five animals. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of IL-8 in co-culture CM on primary growth. A Schematic showing the experimental design for subcutaneous transplantation of OS cells. 143B-Luc cells (2 × 106/mouse) were subcutaneously transplanted in 6–7-week-old BALB/c nu/nu mice, and DMEM or co-culture CM was injected into the para-tumor thrice per week. B Tumor volume with or without co-culture CM measured thrice per week after tumor transplantation. C, D Tumor weight and image of the excised tumor with or without co-cultured CM 14 days after tumor transplantation. E, F Immunohistochemistry and quantification of Ki-67 and phospho-FAK in tumor sections with or without co-culture CM 14 days after tumor transplantation. Two fields of view per sample were randomly selected, and quantification was performed in 10 fields. E Ki-67-positive cells in the field were counted and indicated as Ki-67 labeling index. Scale bars represent 50 μm. G Schematic showing the experimental design for subcutaneous OS transplantation using anti-IL-8 antibodies (IL-8 Abs). H Tumor volume after pretreatment with co-culture CM with or without IL-8 Abs (10 µg/mouse) measured thrice per week after tumor transplantation. I and J Weight and image of the excised tumor pretreated with co-culture CM with or without IL-8 Abs 14 days after tumor transplantation. K, L Immunohistochemistry and quantification of Ki-67 and phospho-FAK in tumor sections with co-culture CM with or without IL-8 Abs 14 days after tumor transplantation. Scale bars represent 50 µm. CM conditioned medium, DMEM Dulbecco’s modified Eagle’s medium, FAK focal adhesion kinase, OS osteosarcoma. Data are presented as mean ± standard deviation; *p < 0.05, **p < 0.01. Each group contained five animals. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

208-IL) was serially diluted and captured by Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

208-IL) was serially diluted and captured by Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC208U-100UG
Alexa Fluor 405	IC208V-100UG
Alexa Fluor 488	IC208G-100UG
Alexa Fluor 532	FAB208X
Alexa Fluor 594	IC208T-100UG
Alexa Fluor 647	IC208R-100UG
Alexa Fluor 700	IC208N-100UG
Alexa Fluor 750	IC208S-100UG
Alexa Fluor Plus 405	FAB208AFP405-100UG
Alexa Fluor Plus 488	FAB208AFP488-100UG
Alexa Fluor Plus 555	FAB208AFP555-100UG
Alexa Fluor Plus 594	FAB208AFP594-100UG
Alexa Fluor Plus 647	FAB208AFP647-100UG
Alexa Fluor Plus 680	FAB208AFP680-100UG
Allophycocyanin	FAB208A
Biotin	FAB208B
CoraFluor 1	FAB208CL1
DyLight 350	FAB208D
DyLight 405	FAB208E
DyLight 488	FAB208K
DyLight 550	FAB208L
DyLight 594	FAB208M
DyLight 650	FAB208W
DyLight 680	FAB208Y
DyLight 755	FAB208Z
Fluorescein	IC208F
HRP	FAB208H
Janelia Fluor 525	FAB208JF525
Janelia Fluor 549	FAB208I
Janelia Fluor 646	FAB208J
Janelia Fluor 669	FAB208JF669
PerCP	FAB208C
Phycoerythrin	IC208P
mFluor Violet 450 SE	FAB208MFV450
mFluor Violet 500 SE	FAB208MFV500
mFluor Violet 610 SE	FAB208MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC208F
Carrier Free	IC208P
Carrier Free	MAB208-SP
Carrier Free	MAB208-500
Carrier Free	IC208V-100UG
Carrier Free	IC208U-100UG
Carrier Free	IC208T-100UG
Carrier Free	IC208S-100UG
Carrier Free	IC208R-100UG
Carrier Free	IC208N-100UG
Carrier Free	IC208G-100UG

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208-IL

Recombinant Human IL-8/CXCL8 Protein

(129 Publications)

10 μg

208-IL

1 μg/lane of Recombinant Human IL-8/CXCL8 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	208-IL-010/CF
Carrier Free	208-IL-050/CF

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AF-208-NA

CXCL8/IL-8 Antibody [Unconjugated]

(4 Reviews)

(26 Publications)

100 μg

AF-208-NA

HAF017). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates and conditioned media of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates and conditioned media of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_lightbox">

<b>Biological Strategies Validation. </b>Simple Western lane view shows PBMC conditioned media untreated (-) or treated (+) with 1 μg/mL LPS for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_thumb" />

HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_lightbox">

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hrs and 10ug/mL LPS for 3 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_thumb" />

208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_lightbox">

208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. " class="big_lightbox">

IL-8/CXCL8 was detected in immersion fixed paraffin-embedded sections of human tonsil (surgically removed due to severe EBV-induced mononucleosis) using Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑208‑NA) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for <a class=

Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. " class="big_thumb" />

208-IL) was serially diluted and captured by Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

208-IL) was serially diluted and captured by Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-208-NAU-100UG
Alexa Fluor 405	AF-208-NAV-100UG
Alexa Fluor 488	AF-208-NAG-100UG
Alexa Fluor 532	AF-208-NAX-100UG
Alexa Fluor 594	AF-208-NAT-100UG
Alexa Fluor 647	AF-208-NAR-100UG
Alexa Fluor 700	AF-208-NAN-100UG
Alexa Fluor 750	AF-208-NAS-100UG
Alexa Fluor Plus 405	AF-208-NAAFP405
Alexa Fluor Plus 488	AF-208-NAAFP488
Alexa Fluor Plus 555	AF-208-NAAFP555
Alexa Fluor Plus 594	AF-208-NAAFP594
Alexa Fluor Plus 647	AF-208-NAAFP647
Alexa Fluor Plus 680	AF-208-NAAFP680
Allophycocyanin	AF-208-NAA
Biotin	BAF208
CoraFluor 1	AF-208-NACL1
DyLight 350	AF-208-NAD
DyLight 405	AF-208-NAE
DyLight 488	AF-208-NAK
DyLight 550	AF-208-NAL
DyLight 594	AF-208-NAM
DyLight 650	AF-208-NAW
DyLight 680	AF-208-NAY
DyLight 755	AF-208-NAZ
FITC	AF-208-NAF
HRP	AF-208-NAH
Janelia Fluor 525	AF-208-NAJF525
Janelia Fluor 549	AF-208-NAI
Janelia Fluor 646	AF-208-NAJ
Janelia Fluor 669	AF-208-NAJF669
PE	AF-208-NAP
PerCP	AF-208-NAC
mFluor Violet 450 SE	AF-208-NAMFV450
mFluor Violet 500 SE	AF-208-NAMFV500
mFluor Violet 610 SE	AF-208-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-208-SP

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HS800

Human IL-8/CXCL8 Quantikine HS ELISA Kit

(39 Publications)

1 Kit

HS800

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	PHS800
HRP	SS800

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	PHS800
Carrier Free	SS800

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P8000

Porcine IL-8/CXCL8 Quantikine ELISA Kit

(35 Publications)

1 Kit

P8000

Reactivity:

Po

Species Glossary

Reactivity: Po

Applications:

Applications:

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NBP2-33819

CXCL8/IL-8 Antibody - BSA Free

(5 Publications)

0.1 ml

NBP2-33819

Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human rectum shows moderate positivity in plasma.

Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human fallopian tube shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human lymph node shows weak to moderate cytoplasmic positivity in a subset of non-germinal center cells.

Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human placenta shows moderate positivity in plasma.

(A, B) mRNA expression changes of acne inflammatory markers in sqRT-PCR. The reduction of inflammatory markers was large in the PDT group in which FDT gel and LED irradiation were applied together, in particular, it was greatest in the group in which LED irradiation was applied for 15 minutes. (C, D) Protein expression changes of acne inflammatory markers as indicated by Western blot analysis. The reduction of inflammatory markers was large in the PDT group in which FDT gel and LED irradiation were applied together; in particular, the greatest change was in the group in which LED irradiation was applied for 15 minutes. (B, D) * indicates p<0.05 statistical significance.IL: interleukin, TNF: tumor necrosis factor, TLR: Toll-like receptor, MMP: matrix metalloproteinase. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39623608), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-33819-25ul

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AB-208-NA

CXCL8/IL-8 Antibody [Unconjugated]

(1 Review)

(13 Publications)

1 mg

AB-208-NA

208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-8/CXCL8 Polyclonal Antibody (Catalog # AB-208-NA). The ND₅₀ is typically 5-20 µg/mL." class="big_lightbox">

208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-8/CXCL8 Polyclonal Antibody (Catalog # AB-208-NA). The ND₅₀ is typically 5-20 µg/mL." alt="Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-8/CXCL8 Polyclonal Antibody (Catalog # AB-208-NA). The ND₅₀ is typically 5-20 µg/mL." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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DY535

Porcine IL-8/CXCL8 DuoSet ELISA, 15 Plate

(17 Publications)

1 Kit (for 15 Plates)

DY535

Reactivity:

Po

Species Glossary

Reactivity: Po

Applications:

Applications:

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Q8000B

Human IL-8/CXCL8 QuantiGlo ELISA Kit

(14 Publications)

1 Kit

Q8000B

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB5351

CXCL8/IL-8 Antibody (105105) [Unconjugated]

(9 Publications)

100 μg

MAB5351

535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351). The ND₅₀ is typically 1-6 µg/mL." class="big_lightbox">

Recombinant Porcine IL-8/CXCL8 (Catalog # <a class=

535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351). The ND₅₀ is typically 1-6 µg/mL." alt="Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351). The ND₅₀ is typically 1-6 µg/mL." class="big_thumb" />

535-IN) was serially diluted and captured by Mouse Anti-Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF535) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

535-IN) was serially diluted and captured by Mouse Anti-Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF535) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Po

Species Glossary

Reactivity: Po

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB5351U-100UG
Alexa Fluor 405	FAB5351V-100UG
Alexa Fluor 488	FAB5351G-100UG
Alexa Fluor 532	FAB5351X-100UG
Alexa Fluor 594	FAB5351T-100UG
Alexa Fluor 647	FAB5351R-100UG
Alexa Fluor 700	FAB5351N-100UG
Alexa Fluor 750	FAB5351S-100UG
Alexa Fluor Plus 405	FAB5351AFP405-100UG
Alexa Fluor Plus 488	FAB5351AFP488-100UG
Alexa Fluor Plus 555	FAB5351AFP555-100UG
Alexa Fluor Plus 594	FAB5351AFP594-100UG
Alexa Fluor Plus 647	FAB5351AFP647-100UG
Alexa Fluor Plus 680	FAB5351AFP680-100UG
Allophycocyanin	FAB5351A
Biotin	FAB5351B
CoraFluor 1	FAB5351CL1
DyLight 350	FAB5351D
DyLight 405	FAB5351E
DyLight 488	FAB5351K
DyLight 550	FAB5351L
DyLight 594	FAB5351M
DyLight 650	FAB5351W
DyLight 680	FAB5351Y
DyLight 755	FAB5351Z
FITC	FAB5351F
HRP	FAB5351H
Janelia Fluor 525	FAB5351JF525
Janelia Fluor 549	FAB5351I
Janelia Fluor 646	FAB5351J
Janelia Fluor 669	FAB5351JF669
PE	FAB5351P
PerCP	FAB5351C
mFluor Violet 450 SE	FAB5351MFV450
mFluor Violet 500 SE	FAB5351MFV500
mFluor Violet 610 SE	FAB5351MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB5351-SP
Carrier Free	MAB5351-500

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

CXCL8 Products By Gene Name

Human IL-8/CXCL8 DuoSet ELISA, 15 Plate

Human IL-8/CXCL8 Quantikine ELISA Kit

CXCL8/IL-8 Antibody (6217) [Unconjugated]

Recombinant Human IL-8/CXCL8 Protein

CXCL8/IL-8 Antibody [Unconjugated]

Human IL-8/CXCL8 Quantikine HS ELISA Kit

Porcine IL-8/CXCL8 Quantikine ELISA Kit

CXCL8/IL-8 Antibody - BSA Free

CXCL8/IL-8 Antibody [Unconjugated]

Porcine IL-8/CXCL8 DuoSet ELISA, 15 Plate

Human IL-8/CXCL8 QuantiGlo ELISA Kit

CXCL8/IL-8 Antibody (105105) [Unconjugated]