145-CR) was serially diluted and captured by Mouse Anti-Human/Primate Cripto 1 Monoclonal Antibody (Catalog # MAB2772) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Primate Cripto 1 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human Cripto 1 (Catalog # 145-CR) was serially diluted and captured by Mouse Anti-Human/Primate Cripto 1 Monoclonal Antibody (Catalog # MAB2772) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Primate Cripto 1 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
![Western Blot: Cripto1 Overexpression Lysate (Adult Normal) [NBL1-16798] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cripto1.](http://images.novusbio.com/fullsize/Cripto1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16798-img0002.jpg "Western Blot: Cripto1 Overexpression Lysate (Adult Normal) [NBL1-16798] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cripto1.")
![Western blot analysis of NCCIT cell lysates with NBP3-32220 at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32220 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32220_-western-blot-639204595238361115.jpg "Western blot analysis of NCCIT cell lysates with NBP3-32220 at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32220 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
