| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97521-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20109-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89884-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26650-50ul | |||
![Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.](http://images.novusbio.com/fullsize/APOJ-Clusterin-Antibody-CLI-9-Western-Blot-NBP1-97521-img0002.jpg "Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.")
![N/A Clusterin/APOJ [Biotin]](http://images.novusbio.com/fullsize2/DATA_Clusterin_DY2747_ELISA_1882.jpg "N/A Clusterin/APOJ [Biotin]")
![Western Blot: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - HeLa(A) (0.03ug/ml), MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml), human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-43B-8 (NBP3-20109) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924288.png "Western Blot: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - HeLa(A) (0.03ug/ml), MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml), human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-43B-8 (NBP3-20109) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-43B-8 (NBP3-20109) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20109, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924248.png)
![Flow Cytometry: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-43B-8 (NBP3-20109, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924262.png)
![Flow Cytometry: Clusterin/APOJ Antibody (013) [NBP2-89884] - Analysis of Human CLU on A549 cells. Cells were treated, stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-013-Flow-Cytometry-NBP2-89884-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Clusterin/APOJ Antibody (013) [NBP2-89884] - Analysis of Human CLU on A549 cells. Cells were treated, stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (013) [NBP2-89884] - Staining of Human CLU in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89884 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89884_rabbit-monoclonal-clusterin-apoj-antibody-013-235202514371330.png)
![Western Blot: Clusterin/APOJ Antibody (PSH04-33) [NBP3-32196] - Western blot analysis of Clusterin/APOJ on different lysates with Rabbit anti-Clusterin/APOJ antibody (NBP3-32196) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: A431 cell lysate Lane 4: PANC-1 cell lysate Lane 5: MCF7 cell lysate Lane 6: T-47D cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 35-80 kDa Exposure time: 1 minute 50 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32196) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32196_rabbit-clusterin-apoj-mab-psh04-33-27820241521244.jpg "Western Blot: Clusterin/APOJ Antibody (PSH04-33) [NBP3-32196] - Western blot analysis of Clusterin/APOJ on different lysates with Rabbit anti-Clusterin/APOJ antibody (NBP3-32196) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: A431 cell lysate Lane 4: PANC-1 cell lysate Lane 5: MCF7 cell lysate Lane 6: T-47D cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 35-80 kDa Exposure time: 1 minute 50 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32196) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Clusterin/APOJ Antibody (PSH04-33) [NBP3-32196] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Clusterin/APOJ antibody (NBP3-32196) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32196) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32196_rabbit-clusterin-apoj-mab-psh04-33-29820241492623.jpg)
![Immunohistochemistry-Paraffin: Clusterin/APOJ Antibody (210) [NBP2-89885] - Staining of human CLU in human appendix with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-210-Immunohistochemistry-Paraffin-NBP2-89885-img0003.jpg "Immunohistochemistry-Paraffin: Clusterin/APOJ Antibody (210) [NBP2-89885] - Staining of human CLU in human appendix with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: Clusterin/APOJ Antibody (210) [NBP2-89885] - Staining of human CLU in human pancreas with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-210-Immunohistochemistry-Paraffin-NBP2-89885-img0001.jpg)
![Immunohistochemistry-Paraffin: Clusterin/APOJ Antibody (210) [NBP2-89885] - Staining of human CLU in human ovarian cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-210-Immunohistochemistry-Paraffin-NBP2-89885-img0002.jpg)
![Immunohistochemistry: Clusterin/APOJ Antibody (2A1) [NBP3-26650] - Image of Clusterin/APOJ Antibody (2A1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26650_rabbit-clusterin-apoj-mab-2a1-262202414471524.jpg "Immunohistochemistry: Clusterin/APOJ Antibody (2A1) [NBP3-26650] - Image of Clusterin/APOJ Antibody (2A1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Clusterin/APOJ Antibody (2A1) [NBP3-26650] - Image of Clusterin/APOJ Antibody (2A1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26650_rabbit-clusterin-apoj-mab-2a1-262202415475621.jpg)
![ELISA: Rat Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-75388] - Samples were spiked with high concentrations of Rat Clusterin/APOJ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75388_rat-clusterin-apoj-elisa-kit-colorimetric-13220241681482.jpg "ELISA: Rat Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-75388] - Samples were spiked with high concentrations of Rat Clusterin/APOJ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Clusterin/APOJ ELISA Kit (Colorimetric) [NBP2-75388] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75388_rat-clusterin-apoj-elisa-kit-colorimetric-432024973638.jpg)
![Western Blot: APOJ / Clusterin Overexpression Lysate (Adult Normal) [NBP2-04577] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for APOJ / Clusterin.](http://images.novusbio.com/fullsize/APOJ-Clusterin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04577-img0001.jpg "Western Blot: APOJ / Clusterin Overexpression Lysate (Adult Normal) [NBP2-04577] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for APOJ / Clusterin.")