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![Western Blot: B7-H3/CD276 Antibody (6A1) [NBP2-37657] - Western blot analysis using CD276 mAb against HEK293 (1) and CD276(AA: 30-130)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-6A1-Western-Blot-NBP2-37657-img0001.jpg "Western Blot: B7-H3/CD276 Antibody (6A1) [NBP2-37657] - Western blot analysis using CD276 mAb against HEK293 (1) and CD276(AA: 30-130)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (6A1) [NBP2-37657] - Analysis of cervical cancer tissues (left) and ovarian cancer tissues (right) using CD276 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-6A1-Immunohistochemistry-NBP2-37657-img0003.jpg)
![Flow Cytometry: B7-H3/CD276 Antibody (6A1) [NBP2-37657] - Analysis of PC-3 cells using CD276 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-6A1-Flow-Cytometry-NBP2-37657-img0002.jpg)
![N/A B7-H3/CD276 [Biotin]](http://images.novusbio.com/fullsize2/DATA_B7H3_DY194905_ELISA_2379.jpg "N/A B7-H3/CD276 [Biotin]")
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB Counterstain: IHC hematoxylin](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunohistochemistry-NBP2-32251-img0002.jpg "Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB Counterstain: IHC hematoxylin")
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody [NBP2-32251] - Samples: FFPE sections of MCF-7 cells (endogenous expression) and CD276 transfected Hek293 cells (over-expression). Antibody: Affinity purified rabbit anti-B7-H3/CD276 (NBP2-32251). Detection: DAB](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunocytochemistry-NBP2-32251-img0003.jpg)
![Immunoprecipitation: B7-H3/CD276 Antibody [NBP2-32251] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-CD276 antibody NBP2-32251 used for IP at 6 ug per reaction. CD276 was also immunoprecipitated by rabbit anti-CD276 antibody BL16383. For blotting immunoprecipitated CD276 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-Immunoprecipitation-NBP2-32251-img0001.jpg)
![Western Blot: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Western blot analysis of B7-H3/CD276 on different lysates with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: LoVo cell lysate Lane 5: U-2 OS cell lysate Lane 6: LNCaP cell lysate Lane 7: SH-SY5Y cell lysate Lane 8: THP-1 cell lysate Lane 9: HCT 116 cell lysate Lane 10: Raji cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32139) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32139_rabbit-b7-h3-cd276-mab-psh02-46-278202415212492.jpg "Western Blot: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Western blot analysis of B7-H3/CD276 on different lysates with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: LoVo cell lysate Lane 5: U-2 OS cell lysate Lane 6: LNCaP cell lysate Lane 7: SH-SY5Y cell lysate Lane 8: THP-1 cell lysate Lane 9: HCT 116 cell lysate Lane 10: Raji cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 57 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32139) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Immunocytochemistry analysis of THP-1 cells labeling B7-H3/CD276 with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-B7-H3/CD276 antibody (NBP3-32139) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32139_rabbit-b7-h3-cd276-mab-psh02-46-2920241048482.jpg)
![Flow Cytometry: B7-H3/CD276 Antibody (PSH02-46) [NBP3-32139] - Flow cytometric analysis of THP-1 cells labeling B7-H3/CD276. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32139, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32139_rabbit-b7-h3-cd276-mab-psh02-46-392024884623.jpg)
![Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (RM335) [NBP2-77415] - Staining of human colon cancer tissue section using NBP2-77415.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-RM335-Immunohistochemistry-NBP2-77415-img0001.jpg "Immunohistochemistry-Paraffin: B7-H3/CD276 Antibody (RM335) [NBP2-77415] - Staining of human colon cancer tissue section using NBP2-77415.")
![Western Blot: B7-H3/CD276 Antibody (RM335) [NBP2-77415] - Western Blot of LNCaP cell lysate using Clone RM335 at a 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-77415_rabbit-monoclonal-b7-h3-cd276-antibody-rm335-24620241245910.png)
![Flow Cytometry: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of MJ18 (NBP2-75195) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75195, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-MJ18-Chimeric-Flow-Cytometry-NBP2-75195-img0002.jpg "Flow Cytometry: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Immunofluorescence staining of fixed mouse splenocytes with anti-B7-H3 (CD276) antibody MJ18. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of MJ18 (NBP2-75195) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing weak membrane staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75195, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody")
![ELISA: B7-H3/CD276 Antibody (MJ18) - Chimeric [NBP2-75195] - Binding curve of anti-B7-H3 antibody MJ18 (NBP2-75195) to mouse B7-H3-Fc fusion protein ELISA Plate coated with mouse B7-H3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using NBP2-75195. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-MJ18-Chimeric-ELISA-NBP2-75195-img0001.jpg)
![Immunohistochemistry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138] - Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-B7-H3/CD276 antibody (NBP3-32138) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32138) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32138_rabbit-b7-h3-cd276-mab-pd00-36-2982024143520.jpg "Immunohistochemistry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138] - Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue with Rabbit anti-B7-H3/CD276 antibody (NBP3-32138) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32138) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Flow Cytometry: B7-H3/CD276 Antibody (PD00-36) [NBP3-32138] - Flow cytometric analysis of HEK-293 cells labeling B7-H3/CD276. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32138, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32138_rabbit-b7-h3-cd276-mab-pd00-36-29202416453234.jpg)
![Western Blot: B7-H3/CD276 Antibody (MM0104-20J12) [NBP2-12048] - Lysates of human gastric cancer cells](http://images.novusbio.com/fullsize/B7-H3-CD276-Antibody-MM0104-20J12-Western-Blot-NBP2-12048-img0001.jpg "Western Blot: B7-H3/CD276 Antibody (MM0104-20J12) [NBP2-12048] - Lysates of human gastric cancer cells")
![SDS-Page: Recombinant Human B7-H3/CD276 Protein [NBP2-59914] - 15% SDS-PAGE](http://images.novusbio.com/fullsize/Recombinant-Human-B7-H3-CD276-Protein-SDS-Page-NBP2-59914-img0001.jpg "SDS-Page: Recombinant Human B7-H3/CD276 Protein [NBP2-59914] - 15% SDS-PAGE")
![Western Blot: B7-H3/CD276 Overexpression Lysate [NBP2-06214] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD276.](http://images.novusbio.com/fullsize/CD276-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06214-img0001.jpg "Western Blot: B7-H3/CD276 Overexpression Lysate [NBP2-06214] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD276.")