| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4670-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1866-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-31106-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47812-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32031-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08976 | |||
![<b>Biological Strategies Validation. </b>Increased CD47 expression in aging and replicative senescence. (A) Quantification of relative CD47 mRNA levels by qPCR. Expression levels of senescent cells were normalized to the respective proliferating control (white bar). Data are representative of three independent experiments. All values are means ± SEM. **P < 0.005, ***P < 0.0005, ****P < 0.0001. Statistically significant differences were determined by unpaired Student’s t test. (B) Representative immunofluorescence images from indicated cell types stained for CD47 (green) and Hoechst (blue). Scale bar: 20 µm, except for 3T3 cells: 50 µm. (C) Whole-cell lysates from indicated cell types were isolated and analyzed by Western blotting for the indicated proteins. Senescence was induced by chemical treatment (palbociclib, except for A549: etopsoside). Shown are representative blots of three independent experiments. GAPDH images are derived from the same blot as Fig. S3 (for Panc1 cells the same GAPDH loading control is additionally used in Fig. 8 G). (D) Representative immunofluorescence images of proliferating or senescent small epithelial cells (SAEC) stained for CD47 (green) and Hoechst (blue). scale bars indicate 20 µm. n = 2–3. (E) Western blot analysis of CD47 expression in SAEC cells. Senescence was induced by chemical treatment (palbociclib). GAPDH was used as loading control. n = 2. GAPDH images are derived from the same blot as Fig. S3. (F) Representative immunofluorescence images of primary lung fibroblast (proliferative or replicative senescent by serially passaging; normal healthy [NHLF] or IPF-derived [IPF]) showing CD47 (green) and Hoechst (blue) staining. scale bars indicate 20 µm. n = 2. (G) Whole-cell lysates from primary NHLF or IPF lung fibroblasts were isolated and analyzed by Western blotting for the indicated proteins. Senescence was induced by replicative passing. Shown are representative blots of three to four independent experiments. Source data are available for this figure: SourceData F7. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36459066), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af4670_human-cd47-affinity-purified-polyclonal-ab-4120241240512.jpg)
![<b>Biological Strategies Validation. </b>Increased CD47 expression in aging and replicative senescence. (A) Quantification of relative CD47 mRNA levels by qPCR. Expression levels of senescent cells were normalized to the respective proliferating control (white bar). Data are representative of three independent experiments. All values are means ± SEM. **P < 0.005, ***P < 0.0005, ****P < 0.0001. Statistically significant differences were determined by unpaired Student’s t test. (B) Representative immunofluorescence images from indicated cell types stained for CD47 (green) and Hoechst (blue). Scale bar: 20 µm, except for 3T3 cells: 50 µm. (C) Whole-cell lysates from indicated cell types were isolated and analyzed by Western blotting for the indicated proteins. Senescence was induced by chemical treatment (palbociclib, except for A549: etopsoside). Shown are representative blots of three independent experiments. GAPDH images are derived from the same blot as Fig. S3 (for Panc1 cells the same GAPDH loading control is additionally used in Fig. 8 G). (D) Representative immunofluorescence images of proliferating or senescent small epithelial cells (SAEC) stained for CD47 (green) and Hoechst (blue). scale bars indicate 20 µm. n = 2–3. (E) Western blot analysis of CD47 expression in SAEC cells. Senescence was induced by chemical treatment (palbociclib). GAPDH was used as loading control. n = 2. GAPDH images are derived from the same blot as Fig. S3. (F) Representative immunofluorescence images of primary lung fibroblast (proliferative or replicative senescent by serially passaging; normal healthy [NHLF] or IPF-derived [IPF]) showing CD47 (green) and Hoechst (blue) staining. scale bars indicate 20 µm. n = 2. (G) Whole-cell lysates from primary NHLF or IPF lung fibroblasts were isolated and analyzed by Western blotting for the indicated proteins. Senescence was induced by replicative passing. Shown are representative blots of three to four independent experiments. Source data are available for this figure: SourceData F7. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36459066), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af4670_human-cd47-affinity-purified-polyclonal-ab-41202412403560.jpg)
![Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] (NBP2-31106) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-31106-img0012.jpg "Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] (NBP2-31106) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Detection of CD47 expression on two hematological cancer cell lines, Jurkat and Raji. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Western-Blot-NBP2-31106-img0008.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human breast cancer tissue stained with CD47 Antibody (B6H12.2). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Immunohistochemistry-Paraffin-NBP2-31106-img0010.jpg)
![Flow Cytometry: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A surface stain was performed on A431 cells with CD47 Antibody [B6H12.2] NBP2-31106PCP (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Flow-Cytometry-NBP2-31106-img0009.jpg)
![Western Blot: CD47 Antibody (B6H12.2) [Azide Free] [NBP2-31106] - Human brain (lane 1) and testis (lane 2) protein was separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and then probed with 2 ug/ml of anti-CD47. CD47 protein was detected using an anti-mouse HRP secondary antibody.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Western-Blot-NBP2-31106-img0004.jpg)
![Immunocytochemistry: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Human trabecular meshwork (primary ocular cells). Image from verified customer review.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Immunocytochemistry-NBP2-31106-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD47 Antibody [B6H12.2] conjugated to Biotin (NBP2-31106B) at 5 ug/ml for 1 hour at room temperature then detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-31106-img0011.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar epithelial cells in the breast section developed specific membrane-cytoplasmic staining.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Immunohistochemistry-Paraffin-NBP2-31106-img0003.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - Tissue section of human normal breast using mouse monoclonal CD47 antibody (clone B6H12.2) at 0.5ug/ml concentration. The ductal/acinar epithelial cells in the breast section developed specific membrane-cytoplasmic staining.](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Immunohistochemistry-Paraffin-NBP2-31106-img0002.jpg)
![Flow (Cell Surface): CD47 Antibody (B6H12.2) - Azide Free [NBP2-31106] - A surface stain was performed on human peripheral blood lymphocytes with CD47 (B6H12.2) antibody NBP2-31106 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].](http://images.novusbio.com/fullsize/CD47-Antibody-B6H12-2-Azide-Free-Flow-Cell-Surface-NBP2-31106-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD47 Antibody (IAP/964 + B6H12.2) [NBP2-44409] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells. CD47 Antibody (IAP/964 + B6H12.2) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/CD47-Antibody-IAP-964-+-B6H12-2-Immunocytochemistry-Immunofluorescence-NBP2-44409-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD47 Antibody (IAP/964 + B6H12.2) [NBP2-44409] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells. CD47 Antibody (IAP/964 + B6H12.2) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot.")
![Immunohistochemistry-Frozen: CD47 Antibody (IAP/964 + B6H12.2) [NBP2-44409] - Human frozen tonsil tissue. Antibody at 1:100. IHC-Fr image submitted from a verified customer review Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/CD47-Antibody-IAP-964-+-B6H12-2-Immunohistochemistry-Frozen-NBP2-44409-img0004.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody [NBP2-32031] - Staining of human fallopian tube shows moderate to strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/CD47-Antibody-Immunohistochemistry-Paraffin-NBP2-32031-img0013.jpg "Immunohistochemistry-Paraffin: CD47 Antibody [NBP2-32031] - Staining of human fallopian tube shows moderate to strong membranous positivity in glandular cells.")
![Immunohistochemistry-Paraffin: CD47 Antibody [NBP2-32031] - Staining of human prostate shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/CD47-Antibody-Immunohistochemistry-Paraffin-NBP2-32031-img0007.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody [NBP2-32031] - Staining of human tonsil shows moderate membranous positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/CD47-Antibody-Immunohistochemistry-Paraffin-NBP2-32031-img0009.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody [NBP2-32031] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CD47-Antibody-Immunohistochemistry-Paraffin-NBP2-32031-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CD47 Antibody (CD47/3019) [NBP3-08975] - Immunofluorescence staining of PFA-fixed MCF-7 cells. CD47 Mouse Monoclonal Antibody (CD47/3019) followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with RedDot.](http://images.novusbio.com/fullsize/CD47-Antibody-CD47-3019-Immunocytochemistry-Immunofluorescence-NBP3-08975-img0004.jpg "Immunocytochemistry/Immunofluorescence: CD47 Antibody (CD47/3019) [NBP3-08975] - Immunofluorescence staining of PFA-fixed MCF-7 cells. CD47 Mouse Monoclonal Antibody (CD47/3019) followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with RedDot.")
![Immunohistochemistry-Paraffin: CD47 Antibody (CD47/3019) [NBP3-08975] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Biotin-conjugated CD47 Mouse Monoclonal Antibody (IAP/3019).](http://images.novusbio.com/fullsize/CD47-Antibody-CD47-3019-Immunohistochemistry-Paraffin-NBP3-08975-img0008.jpg)
![Flow Cytometry: CD47 Antibody (CD47/3019) [NBP3-08975] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using CD47 Mouse Monoclonal Antibody (CD47/3019) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/CD47-Antibody-CD47-3019-Flow-Cytometry-NBP3-08975-img0005.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody (CD47/3019) [NBP3-08975] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD47 Mouse Monoclonal Antibody (CD47/3019).](http://images.novusbio.com/fullsize/CD47-Antibody-CD47-3019-Immunohistochemistry-Paraffin-NBP3-08975-img0003.jpg)
![Immunohistochemistry-Paraffin: CD47 Antibody (CD47/3019) [NBP3-08975] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD47 Mouse Monoclonal Antibody (CD47/3019).](http://images.novusbio.com/fullsize/CD47-Antibody-CD47-3019-Immunohistochemistry-Paraffin-NBP3-08975-img0006.jpg)
![Protein Array: CD47 Antibody (CD47/3019) [NBP3-08975] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CD47 Mouse Monoclonal Antibody (CD47/3019).](http://images.novusbio.com/fullsize/CD47-Antibody-CD47-3019-Protein-Array-NBP3-08975-img0007.jpg)
![Western Blot: CD47 Antibody (BLR131H) [NBP3-14713] - Whole cell lysate (2 ug) from Jurkat, RKO, GaMG, U2OS, OVCAR-3, HeLa, HEK293T, and A-549 cells prepared using NETN lysis buffer. Rabbit anti-CD47 recombinant monoclonal antibody used at 1:1000. secondary HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/CD47-Antibody-BLR131H-Western-Blot-NBP3-14713-img0001.jpg "Western Blot: CD47 Antibody (BLR131H) [NBP3-14713] - Whole cell lysate (2 ug) from Jurkat, RKO, GaMG, U2OS, OVCAR-3, HeLa, HEK293T, and A-549 cells prepared using NETN lysis buffer. Rabbit anti-CD47 recombinant monoclonal antibody used at 1:1000. secondary HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunocytochemistry: CD47 Antibody (BLR131H) [NBP3-14713] - Whole cell lysate (2 ug) from Jurkat, RKO, GaMG, U2OS, OVCAR-3, HeLa, HEK293T, and A-549 cells prepared using NETN lysis buffer.](http://images.novusbio.com/fullsize/CD47-Antibody-BLR131H-Immunocytochemistry-NBP3-14713-img0003.jpg)
![Immunohistochemistry: CD47 Antibody (BLR131H) [NBP3-14713] - Whole cell lysate (2 ug) from Jurkat, RKO, GaMG, U2OS, OVCAR-3, HeLa, HEK293T, and A-549 cells prepared using NETN lysis buffer. secondary Rabbit anti-CD47 recombinant monoclonal antibody used at 1:1000.](http://images.novusbio.com/fullsize/CD47-Antibody-BLR131H-Immunohistochemistry-NBP3-14713-img0002.jpg)
![Simple Western: CD47 Antibody (BLR131H) [NBP3-14713] - Detection of human CD47 by Simple Western. Samples: Whole cell lysate (0.4 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD47 recombinant monoclonal antibody [BLR131H] (NBP3-14713) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp3-14713_rabbit-cd47-mab-simple-western-303202311194.jpg)
![N/A CD47 [Biotin]](http://images.novusbio.com/fullsize2/DATA_CD47_DY467005_ELISA_2465.jpg "N/A CD47 [Biotin]")
