Products Related to Gene-ID: 961

F0113). Quadrant markers were set based on isotype control antibody (Catalog # MAB005, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0113). Quadrant markers were set based on isotype control antibody (Catalog # MAB005, data not shown). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) mouse CD47 or (B) irrelevant protein, and eGFP was stained with Rat Anti-Mouse CD47 Monoclonal Antibody (Catalog # MAB18661) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on isotype control antibody (Catalog # MAB005, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

1866-CD) to immobilized Recombinant Mouse SIRPa/CD172a Fc Chimera (Catalog # 7154-SA) coated at 1 µg/mL (100 µL/well). At 5 µg/mL, this antibody will block >90% of the binding." class="big_lightbox"> 1866-CD) to immobilized Recombinant Mouse SIRPa/CD172a Fc Chimera (Catalog # 7154-SA) coated at 1 µg/mL (100 µL/well). At 5 µg/mL, this antibody will block >90% of the binding." class="big_thumb" />

4546-SA) is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human CD47 Fc Chimera Avi-tag (Catalog # AVI4670) binds with an ED₅₀ of 6-48 ng/mL." class="big_lightbox"> 4546-SA) is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human CD47 Fc Chimera Avi-tag (Catalog # AVI4670) binds with an ED₅₀ of 6-48 ng/mL." alt="When Recombinant Human SIRP alpha/CD172a Fc Chimera (4546-SA) is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human CD47 Fc Chimera Avi-tag (Catalog # AVI4670) binds with an ED₅₀ of 6-48 ng/mL." class="big_thumb" />

9378-SA) was measured at a concentration range between 6.85 nM and 3.51 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.134 µM. " class="big_lightbox"> 9378-SA) was measured at a concentration range between 6.85 nM and 3.51 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.134 µM. " class="big_thumb" />

F0113). Quadrant markers were set based Rat IgG1 Isotype Control Antibody staining (Catalog # MAB005, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0113). Quadrant markers were set based Rat IgG1 Isotype Control Antibody staining (Catalog # MAB005, data not shown). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with (A) CD47 or (B) irrelevant protein, and eGFP were stained with Rat Anti-Mouse CD47 Monoclonal Antibody (Catalog # MAB1866) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based Rat IgG1 Isotype Control Antibody staining (Catalog # MAB005, data not shown). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

4546-SA) is immobilized at 0.2 µg/mL (100 µL/well), Recombinant Human CD47 Mouse IgG2a Fc Chimera Protein (Catalog # 10673-CD) binds with an ED₅₀ of 30.0-300 ng/mL." class="big_lightbox"> 4546-SA) is immobilized at 0.2 µg/mL (100 µL/well), Recombinant Human CD47 Mouse IgG2a Fc Chimera Protein (Catalog # 10673-CD) binds with an ED₅₀ of 30.0-300 ng/mL." class="big_thumb" />

4546-SA) is immobilized at 2 µg/mL, Recombinant Human CD47 (Catalog # 9396-CD) binds with an ED₅₀ of 0.04-0.2 µg/mL." class="big_lightbox"> 4546-SA) is immobilized at 2 µg/mL, Recombinant Human CD47 (Catalog # 9396-CD) binds with an ED₅₀ of 0.04-0.2 µg/mL." alt="When Recombinant Human SIRPa/CD172a Fc Chimera (Catalog # 4546-SA) is immobilized at 2 µg/mL, Recombinant Human CD47 (Catalog # 9396-CD) binds with an ED₅₀ of 0.04-0.2 µg/mL." class="big_thumb" />