RANK/TNFRSF11A: Novus Biologicals

NB100-56508

RANK/TNFRSF11A Antibody (9A725) - BSA Free

(20 Publications)

0.1 mg

NB100-56508

Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Intracellular flow cytometry analysis of RANK in 10^6 RAW cells using 2 ug of RANK antibody. Shaded histogram represents RAW cells without antibody; green represents isotype control; antibody red represents RANK antibody.

Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Using the PE direct conjugate An intracellular stain was performed on K-562 cells with RANK/TNFRSF11A (9A725) antibody NB100-56029 (blue) and a matched isotype control NBP1-97005PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A [9A725] Antibody NB100-56508AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) - BSA Free [NB100-56508] - An intracellular stain was performed on MG-63 cells with RANK/TNFRSF11A Antibody (9A725) NB100-56508 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).

RANK/TNFRSF11A (9A725) was detected in immersion fixed Raw 264.7 mouse macrophage cell line using Mouse anti-RANK/TNFRSF11A (9A725) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB100-56508AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Analysis of a FFPE tissue section of human brain using 1:200 dilution of RANK/TNFRSF11A Antibody (9A725) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Circ-0110634 bound to TNFRII and TRAF2 (A) Potential proteins which could interact with TNFRII (B) The interaction between these proteins and TNFRII in HEK-293T cells with pcDNA3.1/circ-0110634 (C) RNA pull down assay was taken to verify whether circ-0110634 could combine with TRAF2 (D–E) RNA-protein pull-down and RIP assays were taken to verify whether circ-0110634 could bind to TRAF2 and TNFRII (F) TNFRII protein was cut down into 6 pieces (G–H) RNA-protein pull-down assays and gel electrophoresis were taken to verify the specific part circ-0110634 could bind to TNFRII (I) TRAF2 protein was cut into 8 pieces (J–K) RNA-protein pull down assays and gel electrophoresis were performed to confirm the binding capacity between specific pieces of TRAF2 and circ-0110634 (L) The binding capacity between TRAF2 and TNFRII was analyzed in circ-0110634-upregulated cells. ∗∗P < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36185580), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Av

Species Glossary

Reactivity: Hu, Mu, Rt, Av

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-128A

Alt Catalog# IMG-128A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56508AF350
Alexa Fluor 405	NB100-56508AF405
Alexa Fluor 488	NB100-56508AF488
Alexa Fluor 532	NB100-56508AF532
Alexa Fluor 594	NB100-56508AF594
Alexa Fluor 647	NB100-56508AF647
Alexa Fluor 700	NB100-56508AF700
Alexa Fluor 750	NB100-56508AF750
Alexa Fluor Plus 405	NB100-56508AFP405
Alexa Fluor Plus 488	NB100-56508AFP488
Alexa Fluor Plus 555	NB100-56508AFP555
Alexa Fluor Plus 594	NB100-56508AFP594
Alexa Fluor Plus 647	NB100-56508AFP647
Alexa Fluor Plus 680	NB100-56508AFP680
Allophycocyanin	NB100-56508APC
Allophycocyanin/Cy7	NB100-56508APCCY7
Biotin	NB100-56508B
CoraFluor 1	NB100-56508CL1
DyLight 350	NB100-56508UV
DyLight 405	NB100-56508V
DyLight 488	NB100-56508G
DyLight 550	NB100-56508R
DyLight 594	NB100-56508DL594
DyLight 650	NB100-56508C
DyLight 680	NB100-56508FR
DyLight 755	NB100-56508IR
FITC	NB100-56508F
HRP	NB100-56508H
Janelia Fluor 525	NB100-56508JF525
Janelia Fluor 549	NB100-56508JF549
Janelia Fluor 646	NB100-56508JF646
Janelia Fluor 669	NB100-56508JF669
PE	NB100-56029
PE/Atto594	NB100-56508PEATT594
PE/Cy5.5	NB100-56508PECY55
PE/Cy7	NB100-56508PECY7
PerCP	NB100-56508PCP
mFluor Violet 450 SE	NB100-56508MFV450
mFluor Violet 500 SE	NB100-56508MFV500
mFluor Violet 610 SE	NB100-56508MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56508SS
Azide and BSA Free	NBP2-33213

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NBP2-24702

RANK/TNFRSF11A Antibody (64C1385.1) - BSA Free

(3 Publications)

0.1 mg

NBP2-24702

Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Total protein from Raw 264.7 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RANK1 in 1% block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

<b>Biological Strategies Validation. </b>Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Analysis of antibody on (a) untreated BALB/c mouse splenocytes and (b) BALB/c mouse splenocytes stimulated with 5 ug/ml ConA for 72 hours. Cells were stained with 2 ug of antibody per 1x10^6 cells. Green represents isotype control; red represents antibody. Image using the PE format of this antibody.

Western Blot: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Detection of RANK in RAW cells. Lane 1. detection of RANK in RAW, a mouse cell line. Lane 2 & 3. IP/analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or this antibody. RANK was detected by western blot using this antibody.

Flow Cytometry: RANK/TNFRSF11A Antibody (64C1385.1) [NBP2-24702] - Analysis on RAW cells using 5 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control; purple represents antibody. Image using the PE format of this antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-325A

Alt Catalog# IMG-325A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24702AF350
Alexa Fluor 405	NBP2-24702AF405
Alexa Fluor 488	NBP2-24702AF488
Alexa Fluor 532	NBP2-24702AF532
Alexa Fluor 594	NBP2-24702AF594
Alexa Fluor 647	NBP2-24702AF647
Alexa Fluor 700	NBP2-24702AF700
Alexa Fluor 750	NBP2-24702AF750
Alexa Fluor Plus 405	NBP2-24702AFP405
Alexa Fluor Plus 488	NBP2-24702AFP488
Alexa Fluor Plus 555	NBP2-24702AFP555
Alexa Fluor Plus 594	NBP2-24702AFP594
Alexa Fluor Plus 647	NBP2-24702AFP647
Alexa Fluor Plus 680	NBP2-24702AFP680
Allophycocyanin	NBP2-24702APC
Biotin	NBP2-24702B
DyLight 350	NBP2-24702UV
DyLight 405	NBP2-24702V
DyLight 488	NBP2-24702G
DyLight 550	NBP2-24702R
DyLight 594	NBP2-24702DL594
DyLight 650	NBP2-24702C
DyLight 680	NBP2-24702FR
DyLight 755	NBP2-24702IR
FITC	NBP2-24702F
HRP	NBP2-24702H
Janelia Fluor 549	NBP2-24702JF549
Janelia Fluor 549	NBP2-33256JF549
Janelia Fluor 646	NBP2-24702JF646
Janelia Fluor 646	NBP2-33256JF646
PE	NBP2-33256PE
PerCP	NBP2-24702PCP

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-24702SS
Azide and BSA Free	NBP2-33256

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AF692

RANK/TNFRSF11A Antibody [Unconjugated]

(15 Publications)

100 μg

AF692

CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

RANK/TNFRSF11A was detected in immersion fixed frozen sections of rat embryo (cartillage primordium, 7 days p.c.) using 10 µg/mL Goat Anti-Mouse RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF692) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="RANK/TNFRSF11A was detected in immersion fixed frozen sections of rat embryo (cartillage primordium, 7 days p.c.) using 10 µg/mL Goat Anti-Mouse RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF692) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Mouse RANK/TNFRSF11A Antibody (Catalog # AF692) induces osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells in the presence of recombinant mouse M-CSF (416ML). The ED<sub>50</sub> for this effect is typically 0.0200 – 0.400 μg/mL.

RANK loss in tumor cells leads to increased CD8+ Tcell tumor infiltration that mediates the delayed tumor latency of RANK−/− tumors. Representative images (b) of CD3+ (n = 4 tumors, ***p = 0.0009) and CD8+ cells (n = 6 tumors, ***p = 0.0001) in RANK+/+ and RANK−/− tumor transplants as assessed by IHC. Scale = 25 μm. Tumors derived from three independent primary tumors were used. Each dot represents one picture#. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33303745), licensed under a CC-BY license. Not internally tested by R&D Systems.

RANKL, RANK, OPG and LGR4 expression patterns in the mandible first molar of 5-day-old wild-type mice. (A) Immunohistochemistry experiments were carried out on 5-µm-thick frontal sections of 5-day-old wild-type C57BL/6 mouse heads for RANKL, RANK, OPG and LGR4. RANKL and OPG expressions were observed in some mesenchymal cells in the pulp, mainly facing Hertwig’s epithelial root sheath, in mesenchymal cells in the apical papilla, and in certain alveolar bone cells (arrowheads). RANK expression was high in the pulp, in Hertwig’s epithelial root sheath and in the cells at the bone surface. Mild LGR4 expression was evidenced in some cells in Hertwig’s epithelial root sheath and the apical papilla (arrowheads), and in most cells at the alveolar bone surface. Scale: 20X/100 µm. (B) Schematic representations of established RANKL, RANK, OPG and LGR4 expression patterns in 5-day-old wild-type C57BL/6 mice mandibular first molar roots. The cells in Hertwig’s epithelial root sheath expressed only RANK and LGR4, with a significant difference in the number of stained cells and the staining intensity in favor of RANK. DE: dental epithelium; E: enamel; D: dentin; P: pulp; HERS: Hertwig’s epithelial root sheath. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32209985), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF692U-100UG
Alexa Fluor 405	AF692V-100UG
Alexa Fluor 488	AF692G-100UG
Alexa Fluor 532	AF692X-100UG
Alexa Fluor 594	AF692T-100UG
Alexa Fluor 647	AF692R-100UG
Alexa Fluor 700	AF692N-100UG
Alexa Fluor 750	AF692S-100UG
Alexa Fluor Plus 405	AF692AFP405-100UG
Alexa Fluor Plus 488	AF692AFP488-100UG
Alexa Fluor Plus 555	AF692AFP555-100UG
Alexa Fluor Plus 594	AF692AFP594-100UG
Alexa Fluor Plus 647	AF692AFP647-100UG
Alexa Fluor Plus 680	AF692AFP680-100UG
Allophycocyanin	AF692A
Biotin	BAF692
CoraFluor 1	AF692CL1
DyLight 350	AF692D
DyLight 405	AF692E
DyLight 488	AF692K
DyLight 550	AF692L
DyLight 594	AF692M
DyLight 650	AF692W
DyLight 680	AF692Y
DyLight 755	AF692Z
FITC	AF692F
HRP	AF692H
Janelia Fluor 525	AF692JF525
Janelia Fluor 549	AF692I
Janelia Fluor 646	AF692J
Janelia Fluor 669	AF692JF669
PE	AF692P
PerCP	AF692C
mFluor Violet 450 SE	AF692MFV450
mFluor Violet 500 SE	AF692MFV500
mFluor Violet 610 SE	AF692MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF692-SP

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MAB6831

RANK/TNFRSF11A Antibody (80707)

(10 Publications)

100 μg

MAB6831

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

RANK/TNFRSF11A was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human RANK/TNFRSF11A Monoclonal Antibody (Catalog # MAB6831) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="RANK/TNFRSF11A was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human RANK/TNFRSF11A Monoclonal Antibody (Catalog # MAB6831) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6831U-100UG
Alexa Fluor 405	FAB6831V-100UG
Alexa Fluor 488	FAB6831G-100UG
Alexa Fluor 532	FAB6831X-100UG
Alexa Fluor 594	FAB6831T-100UG
Alexa Fluor 647	FAB6831R-100UG
Alexa Fluor 700	FAB6831N-100UG
Alexa Fluor 750	FAB6831S-100UG
Alexa Fluor Plus 405	FAB6831AFP405-100UG
Alexa Fluor Plus 488	FAB6831AFP488-100UG
Alexa Fluor Plus 555	FAB6831AFP555-100UG
Alexa Fluor Plus 594	FAB6831AFP594-100UG
Alexa Fluor Plus 647	FAB6831AFP647-100UG
Alexa Fluor Plus 680	FAB6831AFP680-100UG
Allophycocyanin	FAB6831A
Biotin	FAB6831B
CoraFluor 1	FAB6831CL1
DyLight 350	FAB6831D
DyLight 405	FAB6831E
DyLight 488	FAB6831K
DyLight 550	FAB6831L
DyLight 594	FAB6831M
DyLight 650	FAB6831W
DyLight 680	FAB6831Y
DyLight 755	FAB6831Z
FITC	FAB6831F
HRP	FAB6831H
Janelia Fluor 525	FAB6831JF525
Janelia Fluor 549	FAB6831I
Janelia Fluor 646	FAB6831J
Janelia Fluor 669	FAB6831JF669
PE	FAB6831P
PerCP	FAB6831C
mFluor Violet 450 SE	FAB6831MFV450
mFluor Violet 500 SE	FAB6831MFV500
mFluor Violet 610 SE	FAB6831MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6831-SP
Carrier Free	MAB6831-500

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AF683

RANK/TNFRSF11A Antibody [Unconjugated]

(5 Publications)

100 μg

AF683

Expression of mRNA encoding tumor necrosis factor receptor superfamily, member 11a (TNFRSF11A, wild type receptor activator of NF-kB, wt RANK), TNFRSF11A_ delta 9 (RANK-a), TNFRSF11A_ delta 8,9 (RANK-b) and TNFRSF11A_ delta 7,8,9 (RANK-c) in breast cancer cell lines and breast cancer samples. A. Agarose gel electrophoresis (2%) of PCR products using primers P1 and P2 depicting TNFRSF11A and the identified alternative splice variants in MDA-MB-231, SKBR3, MCF7, MDA-MB-468, T47D and MCF10A breast cancer cell lines. vTNFRSF11A_exon9a was observed only in SKBR3 and after cloning and sequencing was identified as a TNFRSF11A variant encompassing exons 6, 7, 8, 9a and 10. Splice variant TNFRSF11A_ delta 7,8,9 is in bold. B. TNFRSF11A_ delta 7,8,9 (RANK-c) relative mRNA expression levels in a panel of breast cancer cell lines and the non-tumorigenic epithelial cell line MCF10A; values obtained for MCF10A were set to 100. C. Relative mRNA expression levels of variant TNFRSF11A_ delta 7,8,9 (RANK-c) in a panel of formaldehyde-fixed paraffin embedded (FFPE) invasive ductal breast carcinoma samples analyzed against tumor histological grade and proliferation index Ki-67, respectively. D. Relative mRNA expression levels of TNFRSF11A (wt RANK) in the same panel of invasive ductal breast carcinoma samples plotted against tumor histological grade. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22824341), licensed under a CC-BY license. Not internally tested by R&D Systems.

Induction of the expression of RANK-L, OPG, and RANK by normal human blood neutrophils incubated in the presence of synovial fluid (SF) from patients with rheumatoid arthritis (RA) or patients with osteoarthritis (OA). (a) Surface expression of RANK-L by normal blood neutrophils incubated in SF from patients with RA (RA-SF) or OA (OA-SF) for 2 days. Flow cytometry was performed after incubation of neutrophils with a goat anti-human RANK-L antibody followed by a fluorescein isothiocyanate (FITC)-conjugated anti-goat F(ab')2 antibody. Control isotype antibody was a normal goat immunoglobulin G (IgG). Results shown are representative of three RA-SF and nine OA-SF. (b) Expression of mRNA for OPG and RANK by normal blood neutrophils incubated for 2 days in SF from four patients with RA and two patients with OA. Total RNA was isolated from freshly isolated normal blood neutrophils (D0) and from neutrophils of the same healthy donors after 2 days of incubation in SF (RA-1 to -4, OA-1, -2). RNA was then analyzed by reverse transcriptase-polymerase chain reaction. Results shown are representative of two different healthy donors. (c) Surface expression of RANK by normal blood neutrophils incubated in SF from patients with RA (RA-SF) or OA (OA-SF) for 3 days. Flow cytometry was performed after cellular fixation, permeabilization, and staining with a mouse monoclonal anti-human RANK antibody followed by a FITC-conjugated anti-mouse F(ab')2 antibody. Control isotype antibody was a non-specific mouse IgG. Results shown are representative of neutrophils from two different healthy subjects incubated in three different RA-SF and two OA-SF. OPG, osteoprotegerin; RANK, receptor activator of nuclear factor-kappa-B; RANK-L, ligand of receptor activator of nuclear factor-kappa-B. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/17341304), licensed under a CC-BY license. Not internally tested by R&D Systems.

683-RK) was serially diluted and captured by capture antibody coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF683) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human RANK/TNFRSF11A Fc Chimera (Catalog # <a class=

683-RK) was serially diluted and captured by capture antibody coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF683) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF683U-100UG
Alexa Fluor 405	AF683V-100UG
Alexa Fluor 488	AF683G-100UG
Alexa Fluor 532	AF683X-100UG
Alexa Fluor 594	AF683T-100UG
Alexa Fluor 647	AF683R-100UG
Alexa Fluor 700	AF683N-100UG
Alexa Fluor 750	AF683S-100UG
Alexa Fluor Plus 405	AF683AFP405-100UG
Alexa Fluor Plus 488	AF683AFP488-100UG
Alexa Fluor Plus 555	AF683AFP555-100UG
Alexa Fluor Plus 594	AF683AFP594-100UG
Alexa Fluor Plus 647	AF683AFP647-100UG
Alexa Fluor Plus 680	AF683AFP680-100UG
Allophycocyanin	AF683A
Biotin	BAF683
CoraFluor 1	AF683CL1
DyLight 350	AF683D
DyLight 405	AF683E
DyLight 488	AF683K
DyLight 550	AF683L
DyLight 594	AF683M
DyLight 650	AF683W
DyLight 680	AF683Y
DyLight 755	AF683Z
FITC	AF683F
HRP	AF683H
Janelia Fluor 525	AF683JF525
Janelia Fluor 549	AF683I
Janelia Fluor 646	AF683J
Janelia Fluor 669	AF683JF669
PE	AF683P
PerCP	AF683C
mFluor Violet 450 SE	AF683MFV450
mFluor Violet 500 SE	AF683MFV500
mFluor Violet 610 SE	AF683MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF683-SP

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683-RK

Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF

(7 Publications)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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692-RK

Recombinant Mouse RANK/TNFRSF11A Fc Chimera Protein, CF

(4 Publications)

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NBP1-85771

RANK/TNFRSF11A Antibody - BSA Free

(2 Publications)

0.1 ml

NBP1-85771

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85771-25ul

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DY683

Human RANK/TNFRSF11A DuoSet ELISA, 15 Plate

(2 Publications)

1 Kit (for 15 Plates)

DY683

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB6832

RANK/TNFRSF11A Antibody (80710) [Unconjugated]

100 μg

MAB6832

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

RANK/TNFRSF11A was detected in immersion fixed PC‑3 Human Prostate Cancer Cell Line (positive) and K562 Human Chronic Myelogenous Leukemia Cell Line (negative) Cells using Mouse Anti-Human RANK/TNFRSF11A Monoclonal Antibody (Catalog # MAB6832) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="RANK/TNFRSF11A was detected in immersion fixed PC‑3 Human Prostate Cancer Cell Line (positive) and K562 Human Chronic Myelogenous Leukemia Cell Line (negative) Cells using Mouse Anti-Human RANK/TNFRSF11A Monoclonal Antibody (Catalog # MAB6832) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

RANK/TNFRSF11A was detected in immersion fixed paraffin-embedded sections of Human Colon using Mouse Anti-Human RANK/TNFRSF11A Monoclonal Antibody (Catalog # MAB6832) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6832U-100UG
Alexa Fluor 405	FAB6832V-100UG
Alexa Fluor 488	FAB6832G-100UG
Alexa Fluor 532	FAB6832X-100UG
Alexa Fluor 594	FAB6832T-100UG
Alexa Fluor 647	FAB6832R-100UG
Alexa Fluor 700	FAB6832N-100UG
Alexa Fluor 750	FAB6832S-100UG
Alexa Fluor Plus 405	FAB6832AFP405-100UG
Alexa Fluor Plus 488	FAB6832AFP488-100UG
Alexa Fluor Plus 555	FAB6832AFP555-100UG
Alexa Fluor Plus 594	FAB6832AFP594-100UG
Alexa Fluor Plus 647	FAB6832AFP647-100UG
Alexa Fluor Plus 680	FAB6832AFP680-100UG
Allophycocyanin	FAB6832A
Biotin	FAB6832B
CoraFluor 1	FAB6832CL1
DyLight 350	FAB6832D
DyLight 405	FAB6832E
DyLight 488	FAB6832K
DyLight 550	FAB6832L
DyLight 594	FAB6832M
DyLight 650	FAB6832W
DyLight 680	FAB6832Y
DyLight 755	FAB6832Z
FITC	FAB6832F
HRP	FAB6832H
Janelia Fluor 525	FAB6832JF525
Janelia Fluor 549	FAB6832I
Janelia Fluor 646	FAB6832J
Janelia Fluor 669	FAB6832JF669
PE	FAB6832P
PerCP	FAB6832C
mFluor Violet 450 SE	FAB6832MFV450
mFluor Violet 500 SE	FAB6832MFV500
mFluor Violet 610 SE	FAB6832MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6832-SP

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NB100-56396

RANK/TNFRSF11A Antibody - BSA Free

0.1 mg

NB100-56396

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5116A

Alt Catalog# IMG-5116A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56396SS

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MAB11492

RANK/TNFRSF11A Antibody (Hu200) [Unconjugated]

100 μg

MAB11492

Direct ELISA binding curve demonstrating the recognition of Human Anti-Human RANK/TNFRSF11A (Research Grade Denosumab Biosimilar) Monoclonal Antibody (Catalog # MAB11492) to RANK/TNFRSF11A. The target protein was coated onto the microplate well surface, followed by binding of the antibody. A goat anti-human HRP conjugate was used for detection.

Biosimilar antibody development at Bio-Techne includes in silico design and engineering to generate both light chain (lc) and heavy chain (hc) sequences for each antibody. After transfection of the dual expression vector, Bio-Techne’s biosimilar antibodies undergo rigorous application testing to ensure the final biosimilar product is suited for your research needs.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Human Monoclonal

Host: Human Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11492U-100UG
Alexa Fluor 405	FAB11492V-100UG
Alexa Fluor 488	FAB11492G-100UG
Alexa Fluor 532	FAB11492X-100UG
Alexa Fluor 594	FAB11492T-100UG
Alexa Fluor 647	FAB11492R-100UG
Alexa Fluor 700	FAB11492N-100UG
Alexa Fluor 750	FAB11492S-100UG
Alexa Fluor Plus 405	FAB11492AFP405-100UG
Alexa Fluor Plus 488	FAB11492AFP488-100UG
Alexa Fluor Plus 555	FAB11492AFP555-100UG
Alexa Fluor Plus 594	FAB11492AFP594-100UG
Alexa Fluor Plus 647	FAB11492AFP647-100UG
Alexa Fluor Plus 680	FAB11492AFP680-100UG
Allophycocyanin	FAB11492A
Biotin	FAB11492B
CoraFluor 1	FAB11492CL1
DyLight 350	FAB11492D
DyLight 405	FAB11492E
DyLight 488	FAB11492K
DyLight 550	FAB11492L
DyLight 594	FAB11492M
DyLight 650	FAB11492W
DyLight 680	FAB11492Y
DyLight 755	FAB11492Z
FITC	FAB11492F
HRP	FAB11492H
Janelia Fluor 525	FAB11492JF525
Janelia Fluor 549	FAB11492I
Janelia Fluor 646	FAB11492J
Janelia Fluor 669	FAB11492JF669
PE	FAB11492P
PerCP	FAB11492C
mFluor Violet 450 SE	FAB11492MFV450
mFluor Violet 500 SE	FAB11492MFV500
mFluor Violet 610 SE	FAB11492MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11492-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

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RANK/TNFRSF11A Antibody [Unconjugated]

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RANK/TNFRSF11A Antibody [Unconjugated]

Recombinant Human RANK/TNFRSF11A Fc Chimera Protein, CF

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RANK/TNFRSF11A Antibody (Hu200) [Unconjugated]