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![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (7T10F5) [NBP3-16288] - Western blot analysis of extracts of various cell lines, using Aldo-keto Reductase 1C3/AKR1C3 Rabbit mAb (NBP3-16288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-7T10F5-Western-Blot-NBP3-16288-img0002.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (7T10F5) [NBP3-16288] - Western blot analysis of extracts of various cell lines, using Aldo-keto Reductase 1C3/AKR1C3 Rabbit mAb (NBP3-16288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody (7T10F5) [NBP3-16288] - Confocal imaging of A549 cells using AKR1C3 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16288_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-7t10f5-immunocytochemistry-immunofluorescence-186202491659..jpg)
![Immunocytochemistry/ Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody (7T10F5) [NBP3-16288] - Confocal imaging of A549 cells using Aldo-keto Reductase 1C3/AKR1C3 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16288_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-7t10f5-52202511361261.jpg)
![Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (JE36-28) [NBP3-32015] - Western blot analysis of Aldo-keto Reductase 1C3/AKR1C3 on different lysates with Rabbit anti-Aldo-keto Reductase 1C3/AKR1C3 antibody (NBP3-32015) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Human kidney tissue lysate (40 ug/Lane) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Lane 6: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 37 kDa Observed band size: 35 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32015) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32015_rabbit-aldo-keto-reductase-1c3-akr1c3-mab-je36-28-27820241514172.jpg "Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody (JE36-28) [NBP3-32015] - Western blot analysis of Aldo-keto Reductase 1C3/AKR1C3 on different lysates with Rabbit anti-Aldo-keto Reductase 1C3/AKR1C3 antibody (NBP3-32015) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Human kidney tissue lysate (40 ug/Lane) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Lane 6: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 37 kDa Observed band size: 35 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32015) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody (4D12) [NBP3-15212] - Immunofluorescence staining of Hela Cells with at Aldo-keto Reductase 1C3/AKR1C3 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-4D12-Immunocytochemistry-Immunofluorescence-NBP3-15212-img0002.jpg "Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody (4D12) [NBP3-15212] - Immunofluorescence staining of Hela Cells with at Aldo-keto Reductase 1C3/AKR1C3 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: Aldo-keto Reductase 1C3/AKR1C3 Antibody (4D12) [NBP3-15212] - Overlay histogram showing A549 cells stained with Aldo-keto Reductase 1C3/AKR1C3 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/Aldo-keto-Reductase-1C3-AKR1C3-Antibody-4D12-Flow-Cytometry-NBP3-15212-img0001.jpg)