CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="LINGO-1 was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human LINGO-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3086) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3086-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57689-25ul | |||
MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B)." alt="CHP-100 human neuro-blastoma cell line was stained with Mouse Anti-Human LINGO-1 Mono-clonal Antibody (Catalog # MAB30861, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3086-SP | |||
![Immunocytochemistry/Immunofluorescence: LINGO-1 Antibody [NBP2-57689] - Staining of human cell line HEK 293 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LINGO-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57689-img0001.jpg "Immunocytochemistry/Immunofluorescence: LINGO-1 Antibody [NBP2-57689] - Staining of human cell line HEK 293 shows localization to plasma membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: LINGO-1 Antibody [NBP2-57689] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/LINGO-1-Antibody-Immunohistochemistry-Paraffin-NBP2-57689-img0005.jpg)
![Immunohistochemistry-Paraffin: LINGO-1 Antibody [NBP2-57689] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/LINGO-1-Antibody-Immunohistochemistry-Paraffin-NBP2-57689-img0003.jpg)
![LINGO1 antibody [C1C3] detects LINGO1 protein by immunofluorescent analysis.</br>Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: LINGO1 stained by LINGO1 antibody [C1C3] (NBP2-19359) diluted at 1:500.</br>Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19359_rabbit-polyclonal-lingo-1-antibody-immunocytochemistry-immunofluorescence-2611202518482321.jpg "LINGO1 antibody [C1C3] detects LINGO1 protein by immunofluorescent analysis.</br>Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: LINGO1 stained by LINGO1 antibody [C1C3] (NBP2-19359) diluted at 1:500.</br>Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.</br>Blue: Fluoroshield with DAPI .")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LINGO1 antibody [C1C3] (NBP2-19359) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19359_rabbit-polyclonal-lingo-1-antibody-western-blot-2611202518191322.jpg)
![Immunohistochemistry-Paraffin: LINGO-1 Antibody - BSA Free [NBP1-39603] - Analysis of anti-LINGO1 antibody with human brain, cortex at concentration 20 ug/ml.](http://images.novusbio.com/fullsize/LINGO-1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-39603-img0001.jpg "Immunohistochemistry-Paraffin: LINGO-1 Antibody - BSA Free [NBP1-39603] - Analysis of anti-LINGO1 antibody with human brain, cortex at concentration 20 ug/ml.")
![Western Blot: LINGO-1 Antibody [NBP1-46335] - WB on brain lysates. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:2000 dilution incubated overnight at 4C.](http://images.novusbio.com/fullsize/LINGO-1-Antibody-Western-Blot-NBP1-46335-img0001.jpg "Western Blot: LINGO-1 Antibody [NBP1-46335] - WB on brain lysates. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:2000 dilution incubated overnight at 4C.")
![Western Blot: Lingo1 Overexpression Lysate (Adult Normal) [NBL1-12545] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lingo1.](http://images.novusbio.com/fullsize/Lingo1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12545-img0002.jpg "Western Blot: Lingo1 Overexpression Lysate (Adult Normal) [NBL1-12545] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lingo1.")
![Immunohistochemistry-Paraffin: LINGO-1 Antibody [NBP3-14392] - Immunohistochemistry of formalin-fixed, paraffin-embedded human hippocampus after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/LINGO-1 Antibody-Immunohistochemistry-Paraffin-NBP3-14392-img0001.jpg "Immunohistochemistry-Paraffin: LINGO-1 Antibody [NBP3-14392] - Immunohistochemistry of formalin-fixed, paraffin-embedded human hippocampus after heat-induced antigen retrieval.")
![SDS-Page: Recombinant Human LINGO-1 Protein [NBP2-52016]](http://images.novusbio.com/fullsize/Recombinant-Human-LINGO-1-Protein-SDS-Page-NBP2-52016-img0002.jpg "SDS-Page: Recombinant Human LINGO-1 Protein [NBP2-52016]")
