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![Immunocytochemistry/ Immunofluorescence: Axin-1 Antibody - BSA Free [NBP1-76483] - Immunofluorescence of Axin-1 in human brain tissue with Axin-1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76483_rabbit-polyclonal-axin-1-antibody-271120242074620.jpg "Immunocytochemistry/ Immunofluorescence: Axin-1 Antibody - BSA Free [NBP1-76483] - Immunofluorescence of Axin-1 in human brain tissue with Axin-1 antibody at 20 u/mL.")
![Western Blot: Axin-1 Antibody [NBP1-76483] - SK-N-SH cell lysate with AXIN1 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/Axin-1-Antibody-Western-Blot-NBP1-76483-img0001.jpg)
![Lane 1: Marker [kDa] 250,130,95,72,55,36,28,17,10Lane 2: RT-4Lane 3: U-251MGLane 4: Human PlasmaLane 5: LiverLane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-25292_rabbit-axin-1-pab-2012202314593078.jpg "Lane 1: Marker [kDa] 250,130,95,72,55,36,28,17,10Lane 2: RT-4Lane 3: U-251MGLane 4: Human PlasmaLane 5: LiverLane 6: Tonsil")
![Western Blot: Axin-1 Antibody (PSH03-00) [NBP3-32064] - Western blot analysis of Axin-1 on different lysates with Rabbit anti-Axin-1 antibody (NBP3-32064) at 1/2,000 dilution. Lane 1: SW480 cell lysate (10 ug/Lane) Lane 2: HeLa cell lysate (10 ug/Lane) Lane 3: 293T cell lysate (10 ug/Lane) Lane 4: K-562 cell lysate (10 ug/Lane) Lane 5: Huh7 cell lysate (10 ug/Lane) Predicted band size: 95.6 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32064) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32064_rabbit-axin-1-mab-psh03-00-278202415242633.jpg "Western Blot: Axin-1 Antibody (PSH03-00) [NBP3-32064] - Western blot analysis of Axin-1 on different lysates with Rabbit anti-Axin-1 antibody (NBP3-32064) at 1/2,000 dilution. Lane 1: SW480 cell lysate (10 ug/Lane) Lane 2: HeLa cell lysate (10 ug/Lane) Lane 3: 293T cell lysate (10 ug/Lane) Lane 4: K-562 cell lysate (10 ug/Lane) Lane 5: Huh7 cell lysate (10 ug/Lane) Predicted band size: 95.6 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32064) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Axin-1 Antibody (PSH03-00) [NBP3-32064] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Axin-1 antibody (NBP3-32064) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32064) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32064_rabbit-axin-1-mab-psh03-00-29820241420995.jpg)