Axin-1: Novus Biologicals

AF3287

Axin-1 Antibody

100 μg

AF3287

HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." class="big_thumb" />

TNKSi treatment can induce axin expression and axin puncta associated with beta -catenin degradation. A. Western blot analysis of different cell lines treated +/- for 24 h with 2.5 μM of the TNKSi XAV939 revealed an increase in axin expression in different cell lines, and rescue of the degradation of beta -catenin in SW480 CRC cells where the wnt pathway is disrupted by APC mutation. beta -actin levels are shown as loading controls. Quantification of band intensities from two separate blots was performed and normalized to actin. The values shown are mean ± SD. B. SW480 cells were treated with 5 μM of the TNKSi G007-LK for 24 h, then immunostained with specific antibodies to detect different components of the beta -catenin degradation complex located at axin puncta. The following combinations of proteins were co-stained: axin and total beta -catenin, APC and Axin, GSK3 beta and Axin and phosphorylated and non-phosphorylated beta -catenin. Cells were imaged using a DeltaVision microscope system. C. SW480 control cells or cells treated with 5 μM of the TNKSi G007-LK for 24 h were stained for fluorescence. In control cells a disperse staining pattern is observed whereas in TNKSi treated cells the TNKS-1 and -2 (red) specifically colocalize at TNKSi induced axin puncta (green) as shown by immunofluorescence microscopy. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26930278), licensed under a CC-BY license. Not internally tested by R&D Systems.

$Tankyrase inhibitors promote inclusion of axin and TNKS into insoluble complexes. A. NIH 3T3 cells were left untreated (- CSK) or washed for 5 min with 0.2% Triton X-100 containing MT-buffer (+CSK) to permeabilize the plasma membrane and remove soluble proteins, before immunolabeling with fluorescent antibodies for axin and tubulin. Axin puncta in untreated cells and after induction by TNKSi were still visible after the detergent extraction, despite a decrease in background staining. This implies that the axin puncta are part of an insoluble pool resistant to extraction. B. To determine if axin and TNKS accrued in an insoluble fraction in vitro after TNKSi treatment in SW480 cells, cells were extracted either with RIPA buffer (left panel) or enriched for the insoluble fraction by extracting cells on the plate with SDS containing sample buffer (right panel). The addition of TNKSi (G007-LK) increased the accumulation of the TNKS-1 and -2 in the insoluble fractions and this was at least partly reduced for TNKS2 and axin following MG132 treatment, indicating that TNKSi might reduce solubility and mobility of axin and TNKS. Quantification of band intensity was measured and values were corrected to the endogenous control beta -actin (a second experiment is shown in S6 Fig). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26930278), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Proteasome inhibitor induces PARylation of TNKSs and TNKSi reduces basal PARylation levels. A. SW480 and HEK293 cells were exposed to combinations of TNKSi (G007-LK) and MG132 treatments similar to that shown in legend to Fig 3. The cells were then lysed and extracts analysed by IP assay using a specific antibody against PAR. PARylated forms of TNKS1/2 were then detected by western blot. The IgG control was negative. Pull-down of PARylated proteins revealed an increase in PARylation of TNKS1 by MG132 treatment, and a decrease of PARylation induced by TNKSi. When combined, the MG132 dominated and caused some induction of PARylated TNKS1 in both SW480 and HEK293 cells. Levels of PARylated TNKS2 (and also axin, not shown) were too low for detection. Quantification of band intensities (mean ± SD) was from two separate experiments. B. Total extract western blot shows similar TNKS levels after different treatments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26930278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Induction of axin puncta by TNKSi is proteasome-dependent. A. SW480 cells were treated with single or combined doses of tankyrase (5 μM G007LK) and proteasome (20 μM MG132) inhibitors. The MG132 was added for 6 h, either simultaneously with G007-LK for a 6 h treatment, or during the last 6 h of a 24 h G007-LK treatment. As shown in the immunofluorescence images, the addition of MG132 caused a reduction in the % of cells with visible TNKSi-induced axin puncta after 6 h treatment. The 6 h MG132 treatment also caused a modest increase in nuclear staining of axin. The later addition of MG132 (at the end of a 24 h G007-LK treatment) caused the induced axin puncta to relocate to the perinuclear region. B. The number of axin puncta per cell were scored by microscopy and categorized (<5, 5–15 or >15 puncta per cell). As shown, TNKSi induced a high number of puncta per cell (more than 80% of cells scored >15 puncta per cell) and this was blocked at 6 h or reduced after 24 h by MG132 treatment. C. A diagram summarizing the effect of G007-LK +/- MG132 on axin pattern and cellular distribution (shown in green). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26930278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Endocytosis inhibitors cause a decrease in the level of Armadillo/ beta -catenin both in stimulated and unstimulated cells. (A) The level of Armadillo increased in S2R+ cells that had been treated with Wingless-conditioned medium (lane 2) or SB-216763 (lane 5). This was prevented by treatment with Dyngo-4a (lanes 3 and 6) or Dynasore (lanes 4 and 7). Lamin, Actin and Syntaxin levels were unaffected. (B) Dyngo-4a reversibly reduced the level of Armadillo in uninduced cells. (C) Dyngo-4a reduced signalling-induced accumulation of beta -catenin in RKO cells. Cells were pre-incubated with SB-216763 to activate signalling and then exposed to a mixture of SB-216763 and Dyngo-4a. The total time of treatment with either drug is indicated. As in Drosophila cells, Dyngo-4a caused a decrease in beta -catenin levels in unstimulated cells. A progressive decrease can be seen after 0.5, 1 and 2 hours of treatment with Dyngo-4a (no SB-216763) in lanes 9–11. (D) The effect of Dyngo-4a and SB-216763 on the level of various components of the Wnt pathway. LRP6, GSK3 beta and CK1 alpha were largely unaffected, whereas the levels of APC and Axin1 dropped markedly 30–60 minutes after treatment with Dyngo-4a. SB-216763 caused an increase in the amount of beta -catenin. This correlated with a decrease in phosphorylated beta -catenin (pT42/S37/S33 beta -catenin; lane 3), as expected because phosphorylated beta -catenin reflects the activity of the degradation complex (Hernández et al., 2012). By contrast, the (mild) decrease in beta -catenin caused by Dyngo-4a (lanes 7 and 8) is paralleled by a similar decrease in phosphorylated beta -catenin, suggesting that Dyngo-4a impacts on the level of beta -catenin through a mechanism that is independent of the destruction complex. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25236598), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3287U-100UG
Alexa Fluor 405	AF3287V-100UG
Alexa Fluor 488	AF3287G-100UG
Alexa Fluor 532	AF3287X-100UG
Alexa Fluor 594	AF3287T-100UG
Alexa Fluor 647	AF3287R-100UG
Alexa Fluor 700	AF3287N-100UG
Alexa Fluor 750	AF3287S-100UG
Alexa Fluor Plus 405	AF3287AFP405-100UG
Alexa Fluor Plus 488	AF3287AFP488-100UG
Alexa Fluor Plus 555	AF3287AFP555-100UG
Alexa Fluor Plus 594	AF3287AFP594-100UG
Alexa Fluor Plus 647	AF3287AFP647-100UG
Alexa Fluor Plus 680	AF3287AFP680-100UG
Allophycocyanin	AF3287A
Biotin	AF3287B
CoraFluor 1	AF3287CL1
DyLight 350	AF3287D
DyLight 405	AF3287E
DyLight 488	AF3287K
DyLight 550	AF3287L
DyLight 594	AF3287M
DyLight 650	AF3287W
DyLight 680	AF3287Y
DyLight 755	AF3287Z
FITC	AF3287F
HRP	AF3287H
Janelia Fluor 525	AF3287JF525
Janelia Fluor 549	AF3287I
Janelia Fluor 646	AF3287J
Janelia Fluor 669	AF3287JF669
PE	AF3287P
PerCP	AF3287C
mFluor Violet 450 SE	AF3287MFV450
mFluor Violet 500 SE	AF3287MFV500
mFluor Violet 610 SE	AF3287MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3287-SP

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NBP2-61695

Axin-1 Antibody (4E9F1) - BSA Free

(1 Publication)

0.1 ml

NBP2-61695

Flow Cytometry: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis of Hela cells using AXIN1 mouse mAb (green) and negative control (red).

Western Blot: Axin-1 Antibody (4E9F1) [NBP2-61695] - Analysis using AXIN1 mAb against HEK293 (1) and AXIN1 (AA: 546-752)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: Axin-1 Antibody (4E9F1) [NBP2-61695] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-61695-0.025ml

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NBP1-76483

Axin-1 Antibody - BSA Free

(1 Publication)

0.1 mg

NBP1-76483

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76483AF350
Alexa Fluor 405	NBP1-76483AF405
Alexa Fluor 488	NBP1-76483AF488
Alexa Fluor 532	NBP1-76483AF532
Alexa Fluor 594	NBP1-76483AF594
Alexa Fluor 647	NBP1-76483AF647
Alexa Fluor 700	NBP1-76483AF700
Alexa Fluor 750	NBP1-76483AF750
Alexa Fluor Plus 405	NBP1-76483AFP405
Alexa Fluor Plus 488	NBP1-76483AFP488
Alexa Fluor Plus 555	NBP1-76483AFP555
Alexa Fluor Plus 594	NBP1-76483AFP594
Alexa Fluor Plus 647	NBP1-76483AFP647
Alexa Fluor Plus 680	NBP1-76483AFP680
Allophycocyanin	NBP1-76483APC
Biotin	NBP1-76483B
CoraFluor 1	NBP1-76483CL1
DyLight 350	NBP1-76483UV
DyLight 405	NBP1-76483V
DyLight 488	NBP1-76483G
DyLight 550	NBP1-76483R
DyLight 594	NBP1-76483DL594
DyLight 650	NBP1-76483C
DyLight 680	NBP1-76483FR
DyLight 755	NBP1-76483IR
FITC	NBP1-76483F
HRP	NBP1-76483H
Janelia Fluor 525	NBP1-76483JF525
Janelia Fluor 549	NBP1-76483JF549
Janelia Fluor 646	NBP1-76483JF646
Janelia Fluor 669	NBP1-76483JF669
PE	NBP1-76483PE
PerCP	NBP1-76483PCP
mFluor Violet 450 SE	NBP1-76483MFV450
mFluor Violet 500 SE	NBP1-76483MFV500
mFluor Violet 610 SE	NBP1-76483MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76483-0.025mg

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H00008312-M01

Axin-1 Antibody (2B11) - Azide and BSA Free

0.1 mg

H00008312-M01

Sandwich ELISA: Axin-1 Antibody (2B11) [H00008312-M01] - Detection limit for recombinant GST tagged AXIN1 is approximately 0.3ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NB100-1011

Axin-1 Antibody

(2 Publications)

0.1 mg

NB100-1011

Reactivity:

Hu, Mu, Rt, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP3-25292

Axin-1 Antibody - BSA Free

100 ul

NBP3-25292

Lane 1: Marker [kDa] 250,130,95,72,55,36,28,17,10<br/>Lane 2: RT-4<br/>Lane 3: U-251MG<br/>Lane 4: Human Plasma<br/>Lane 5: Liver<br/>Lane 6: Tonsil

Staining of human cell line SiHa shows localization to nucleoli.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-32064

Axin-1 Antibody (PSH03-00)

100 ul

NBP3-32064

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-10887

Axin-1 Overexpression Lysate

0.1 mg

NBP2-10887

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-04933

Axin-1 Overexpression Lysate

0.1 mg

NBP2-04933

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP3-25292PEP

Axin-1 Recombinant Protein Antigen

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP1-76483PEP

Axin-1 Antibody Blocking Peptide

0.05 mg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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AF3287AFP488

Axin-1 Antibody [Alexa Fluor™ Plus 488]

100 μg

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 488

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Products Related to Gene-ID: 8312

Axin-1 Antibody

Axin-1 Antibody (4E9F1) - BSA Free

Axin-1 Antibody - BSA Free

Axin-1 Antibody (2B11) - Azide and BSA Free

Axin-1 Antibody

Axin-1 Antibody - BSA Free

Axin-1 Antibody (PSH03-00)

Axin-1 Overexpression Lysate

Axin-1 Overexpression Lysate

Axin-1 Recombinant Protein Antigen

Axin-1 Antibody Blocking Peptide

Axin-1 Antibody [Alexa Fluor™ Plus 488]