HAF109). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-3 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 2." class="big_lightbox">
HAF109). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-3 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 2." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TRAF-3 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3278) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (
HAF109). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-3 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 2." class="big_thumb" />
HAF005). A specific band was detected for TRAF-3 Isoform 2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat TRAF-3 Isoform 2 Monoclonal Antibody (Catalog # MAB3278) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TRAF-3 Isoform 2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
HAF109). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-3 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TRAF-3 Isoform 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3278) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-3 was detected at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
![Immunohistochemistry-Paraffin: TRAF-3 Antibody - Unpurified [NB100-56176] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using this at 1:4000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Immunohistochemistry-Paraffin-NB100-56176-img0001.jpg "Immunohistochemistry-Paraffin: TRAF-3 Antibody - Unpurified [NB100-56176] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using this at 1:4000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.")
![Western Blot: TRAF-3 Antibody [NB100-56654] - Analysis of TRAF3 in (A) HeLa and (B) NIH 3T3 cell lysate using the TRAF3 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Western-Blot-NB100-56654-img0006.jpg "Western Blot: TRAF-3 Antibody [NB100-56654] - Analysis of TRAF3 in (A) HeLa and (B) NIH 3T3 cell lysate using the TRAF3 antibody at 2 ug/ml.")
![Immunohistochemistry-Paraffin: TRAF-3 Antibody [NB100-56654] - Analysis of human pancreas using TRAF3 antibody at 1:50.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Immunohistochemistry-Paraffin-NB100-56654-img0005.jpg)
![Western Blot: TRAF-3 Antibody [NBP1-76642] - HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/ml.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Western-Blot-NBP1-76642-img0001.jpg "Western Blot: TRAF-3 Antibody [NBP1-76642] - HeLa cell lysate with TRAF3 antibody at (A) 2 and (B) 4 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: TRAF-3 Antibody - BSA Free [NBP1-76642] - Immunocytochemistry of TRAF-3 in HeLa cells with TRAF-3 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76642_rabbit-polyclonal-traf-3-antibody-271120242016971.jpg)
![Immunohistochemistry-Paraffin: TRAF-3 Antibody [NBP1-86639] - Staining of human pancreas shows strong cytoplasmic positivity in granular pattern in exocrine glandular cells.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Immunohistochemistry-Paraffin-NBP1-86639-img0001.jpg "Immunohistochemistry-Paraffin: TRAF-3 Antibody [NBP1-86639] - Staining of human pancreas shows strong cytoplasmic positivity in granular pattern in exocrine glandular cells.")
![Western Blot: TRAF-3 Antibody (PSH01-30) [NBP3-33042] - Western blot analysis of TRAF-3 on different lysates with Rabbit anti-TRAF-3 antibody (NBP3-33042) at 1/1,000 dilution.<br/><br/> Lane 1: TF-1 cell lysate (20 ug/Lane)<br/> Lane 2: THP-1 cell lysate (20 ug/Lane)<br/> Lane 3: 293T cell lysate (20 ug/Lane)<br/> Lane 4: Jurkat cell lysate (20 ug/Lane)<br/> Lane 5: A549 cell lysate (20 ug/Lane)<br/> Lane 6: RAW264.7 cell lysate (20 ug/Lane)<br/> Lane 7: NIH/3T3 cell lysate (20 ug/Lane)<br/> Lane 8: Mouse brain tissue lysate (40 ug/Lane)<br/> Lane 9: Rat spleen tissue lysate (40 ug/Lane)<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 64 kDa<br/> Observed band size: 50 kDa<br/><br/> Exposure time: 1 minute 9 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33042) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33042_rabbit-traf-3-mab-psh01-30-288202412493419.jpg "Western Blot: TRAF-3 Antibody (PSH01-30) [NBP3-33042] - Western blot analysis of TRAF-3 on different lysates with Rabbit anti-TRAF-3 antibody (NBP3-33042) at 1/1,000 dilution.<br/><br/> Lane 1: TF-1 cell lysate (20 ug/Lane)<br/> Lane 2: THP-1 cell lysate (20 ug/Lane)<br/> Lane 3: 293T cell lysate (20 ug/Lane)<br/> Lane 4: Jurkat cell lysate (20 ug/Lane)<br/> Lane 5: A549 cell lysate (20 ug/Lane)<br/> Lane 6: RAW264.7 cell lysate (20 ug/Lane)<br/> Lane 7: NIH/3T3 cell lysate (20 ug/Lane)<br/> Lane 8: Mouse brain tissue lysate (40 ug/Lane)<br/> Lane 9: Rat spleen tissue lysate (40 ug/Lane)<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 64 kDa<br/> Observed band size: 50 kDa<br/><br/> Exposure time: 1 minute 9 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33042) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Western blot analysis on different lysates with NBP3-33042 at 1/1,000 dilution. Lane 1: TF-1 cell lysate (20 µg/Lane) Lane 2: THP-1 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: A549 cell lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 50 kDa Exposure time: 1 minute 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33042 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33042_-western-blot-639204595241755272.jpg)
