| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77044-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF9301-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2129-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB21291-SP | |||
| Carrier Free | FAB21291V-100UG | |||
| Carrier Free | FAB21291U-100UG | |||
| Carrier Free | FAB21291T-100UG | |||
| Carrier Free | FAB21291S-100UG | |||
| Carrier Free | FAB21291R-100UG | |||
| Carrier Free | FAB21291N-100UG | |||
| Carrier Free | FAB21291G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF930-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9302-SP | |||
![Western Blot: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of ADAM17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysateLane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Western-Blot-NBP2-67179-img0006.jpg "Western Blot: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of ADAM17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysateLane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Staining ADAM17 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Immunocytochemistry-Immunofluorescence-NBP2-67179-img0005.jpg)
![Flow Cytometry: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Analysis of Hela cells with ADAM17 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Flow-Cytometry-NBP2-67179-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Staining ADAM17 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Immunocytochemistry-Immunofluorescence-NBP2-67179-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Staining ADAM17 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-JM10-35-Immunocytochemistry-Immunofluorescence-NBP2-67179-img0003.jpg)
![Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] - Rat hippocampus tissue with Rabbit anti-ADAM17 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67179_rabbit-monoclonal-tace-adam17-antibody-jm10-35-immunohistochemistry-paraffin-2272024112433..jpg)
![Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] -Mouse hippocampus tissue with Rabbit anti-ADAM17 antibody at 1/500 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67179_rabbit-monoclonal-tace-adam17-antibody-jm10-35-immunohistochemistry-paraffin-2272024112623..jpg)
![IHC-Frozen: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] -Analysis of frozen mouse cerebral cortex tissue labeling ADAM17 with Rabbit anti-ADAM17 antibody.The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.](http://images.novusbio.com/fullsize/nbp2-67179_rabbit-monoclonal-tace-adam17-antibody-jm10-35-immunohistochemistry-frozen-2272024112828..jpg)
![IHC-Frozen: TACE/ADAM17 Antibody (JM10-35) [NBP2-67179] -Analysis of frozen mouse hippocampus tissue labeling ADAM17 with Rabbit anti-ADAM17 antibody.The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.](http://images.novusbio.com/fullsize/nbp2-67179_rabbit-monoclonal-tace-adam17-antibody-jm10-35-immunohistochemistry-frozen-2272024112950..jpg)
![N/A TACE/ADAM17 [Biotin]](http://images.novusbio.com/fullsize2/DATA_TACE_DY930_ELISA_2267.jpg "N/A TACE/ADAM17 [Biotin]")
![Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TACE KO celllysates. Antibodies: TACE/ADAM17 NBP1-77044 (0.25 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP1-77044-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysates or TACE KO celllysates. Antibodies: TACE/ADAM17 NBP1-77044 (0.25 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - HeLa whole cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP1-77044-img0003.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - Analysis of TACE in HeLa (A, D), Jurkat (B, E) and Raji (C,F) cell lysate in (A-C) the absence or (D-F) the presence of blocking peptide with TACE antibody at 0.5 ug/mL.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP1-77044-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TACE/ADAM17 Antibody [NBP1-77044] - IAV via Protein Expression Profile in Cells Lines. Antibodies: TACE and GAPDH. Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP1-77044-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody - BSA Free [NBP1-77044] - Figure 5 Immunocytochemistry Validation of TACE/ADAM17 in HeLa CellsImmunohistochemical analysis of HeLa cells using anti-TACE/ADAM17 antibody at 10 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77044_rabbit-polyclonal-tace-adam17-antibody-2711202419201577.jpg)
![Immunocytochemistry/ Immunofluorescence: TACE/ADAM17 Antibody - BSA Free [NBP1-77044] - Figure 4 Immunofluorescence Validation of TACE/ADAM17 in HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE/ADAM17 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).](http://images.novusbio.com/fullsize/nbp1-77044_rabbit-polyclonal-tace-adam17-antibody-271120242045673.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP2-15281-img0005.jpg "Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500.")
![Immunohistochemistry-Paraffin: TACE/ADAM17 Antibody [NBP2-15281] - Human breast carcinoma dilution: 1:250. ADAM17 antibody [C2C3], C-term dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Immunohistochemistry-Paraffin-NBP2-15281-img0006.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - 30 ug K562 whole cell lysate/extract, 7.5% SDS-PAGE gel, dilution 1:500.](http://images.novusbio.com/fullsize/ADAM17-Antibody-Western-Blot-NBP2-15281-img0001.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/TACE-ADAM17-Antibody-Western-Blot-NBP2-15281-img0004.jpg)
![Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TACE/ADAM17 antibody [C2C3], C-term (NBP2-15281) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15281_rabbit-polyclonal-tace-adam17-antibody-91020242014683.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TACE/ADAM17 Antibody [NBP2-15281] - Wild-type (WT) and ADAM17 knockout (KO) HeLa (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ADAM17 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15281_rabbit-polyclonal-tace-adam17-antibody-1610202419414541.jpg)
