NL007) and counterstained with DAPI (blue). Specific staining was localized to synapses. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Synaptotagmin-1 was detected in immersion fixed rat hippocampal neurons (E18) using Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB4364) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to synapses. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4364-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34215-25ul | |||
Fluorescent IHC Staining of Frozen Tissue Sections." alt="Synaptotagmin-1 was detected in perfusion fixed frozen sections of rat spinal cord using 8 µg/mL Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB43641) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB43641-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87362-25ul | |||
Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />
0695/50) prior to immersion fixation. Synaptotagmin-1 was detected using a Mouse Anti-Rat Synaptotagmin-1 Monoclonal Antibody (Catalog # MAB4364). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Mouse IgG Affinity-purified Secondary Antibody (red; Catalog # 
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Analysis in human cerebral cortex and pancreas tissues using NBP2-34215 antibody. Corresponding Synaptotagmin 1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Analysis in human cerebral cortex and pancreas tissues using NBP2-34215 antibody. Corresponding Synaptotagmin 1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human cerebellum, liver, pancreas and testis using Anti-Synaptotagmin 1 antibody NBP2-34215 (A) shows similar protein distribution across tissues to independent antibody NBP1-87362 (B).](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0017.jpg)
![Western Blot: Synaptotagmin 1 Antibody [NBP2-34215] - Analysis in control (vector only transfected HEK293T lysate) and SYT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Western-Blot-NBP2-34215-img0011.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0008.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human liver.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0013.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human testis.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0014.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human cerebral cortex using Anti-SYT1 antibody NBP2-34215.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0015.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human pancreas using Anti-SYT1 antibody NBP2-34215.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0016.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249383145.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-212024943559.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249403847.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249371547.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Human tissue extract (5 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249415950.jpg)
![Synaptotagmin 1 antibody [HL1654] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded rat eye.</br>Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:250.</br>Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.</br>Blue: Fluoroshield with DAPI .</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-immunohistochemistry-paraffin-14112025183373.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-212024941483.jpg "Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-21202494082.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-2120249383198.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Human tissue extract (5 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-2120249415927.jpg)
![Synaptotagmin 1 antibody [HL1626] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded rat eye.</br>Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:250.</br>Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.</br>Blue: Fluoroshield with DAPI .</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-immunohistochemistry-paraffin-1411202518381714.jpg)
![Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Western-Blot-NB300-236-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Synaptotagmin-1 [p Ser309] Antibody [NB300-236] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Immunohistochemistry-NB300-236-img0002.jpg)
![Western Blot: Synaptotagmin 1 [p Thr202] Antibody [NB300-235] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Thr202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Thr202 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Thr202]-Antibody-Western-Blot-NB300-235-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Synaptotagmin 1 [p Thr202] Antibody [NB300-235] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Thr202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Thr202 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Synaptotagmin-1 [p Thr202] Antibody [NB300-235] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Thr202 in red and PSD95 in green. Photo courtesy of Gang Liu.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Thr202]-Antibody-Immunohistochemistry-NB300-235-img0002.jpg)
![Western Blot: Synaptotagmin 1 Antibody [NBP1-87362] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Western-Blot-NBP1-87362-img0005.jpg "Western Blot: Synaptotagmin 1 Antibody [NBP1-87362] - Analysis in human cerebral cortex tissue.")
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP1-87362] - Analysis in human cerebral cortex and pancreas tissues using NBP1-87362 antibody. Corresponding Synaptotagmin 1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87362-img0007.jpg)
![Recombinant Human Synaptotagmin-1 Protein [H00006857-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006857-P01-1.jpg "Recombinant Human Synaptotagmin-1 Protein [H00006857-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Mouse Synaptotagmin 1 ELISA Kit (Colorimetric) [NBP2-80287] - Samples were spiked with high concentrations of Mouse Synaptotagmin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80287_mouse-synaptotagmin-1-elisa-kit-colorimetric-132202416175696.jpg "ELISA: Mouse Synaptotagmin 1 ELISA Kit (Colorimetric) [NBP2-80287] - Samples were spiked with high concentrations of Mouse Synaptotagmin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Synaptotagmin 1 ELISA Kit (Colorimetric) [NBP2-80287] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80287_mouse-synaptotagmin-1-elisa-kit-colorimetric-432024955032.jpg)
![Western Blot: Synaptotagmin 1 Antibody [NB300-739] - Western blot of rat cortex showing specific labeling of the ~65k Synaptotagmin protein.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Western-Blot-NB300-739-img0001.jpg "Western Blot: Synaptotagmin 1 Antibody [NB300-739] - Western blot of rat cortex showing specific labeling of the ~65k Synaptotagmin protein.")
![Western Blot: Synaptotagmin 1 Overexpression Lysate (Native) [NBP2-09584] - Synaptotagmin 1 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SYT1.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Overexpression-Lysate-Native-Western-Blot-NBP2-09584-img0002.jpg "Western Blot: Synaptotagmin 1 Overexpression Lysate (Native) [NBP2-09584] - Synaptotagmin 1 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SYT1.")
