| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4364-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34215-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB43641-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87362-25ul | |||
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249383145.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-212024943559.jpg)
![Immunocytochemistry/Immunofluorescence: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Synaptotagmin 1 antibody [HL1654] detects Synaptotagmin 1 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-212024935554.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249403847.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249371547.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Human tissue extract (5 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249415950.jpg)
![Immunocytochemistry/Immunofluorescence: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Synaptotagmin 1 antibody [HL1654] detects Synaptotagmin 1 protein by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249342736.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Analysis in human cerebral cortex and pancreas tissues using NBP2-34215 antibody. Corresponding Synaptotagmin 1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Analysis in human cerebral cortex and pancreas tissues using NBP2-34215 antibody. Corresponding Synaptotagmin 1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human cerebellum, liver, pancreas and testis using Anti-Synaptotagmin 1 antibody NBP2-34215 (A) shows similar protein distribution across tissues to independent antibody NBP1-87362 (B).](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0017.jpg)
![Western Blot: Synaptotagmin 1 Antibody [NBP2-34215] - Analysis in control (vector only transfected HEK293T lysate) and SYT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Western-Blot-NBP2-34215-img0011.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0008.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human liver.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0013.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human testis.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0014.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human cerebral cortex using Anti-SYT1 antibody NBP2-34215.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0015.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human pancreas using Anti-SYT1 antibody NBP2-34215.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0016.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-212024941483.jpg "Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-21202494082.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-2120249383198.jpg)
![Immunocytochemistry/Immunofluorescence: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Synaptotagmin 1 antibody [HL1626] detects Synaptotagmin 1 protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-2120249403816.jpg)
![Immunocytochemistry/Immunofluorescence: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Synaptotagmin 1 antibody [HL1626] detects Synaptotagmin 1 protein by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-212024939179.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1626) - Azide and BSA Free [NBP3-25705] - Human tissue extract (5 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1626] (NBP3-25705) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25705_rabbit-synaptotagmin-1-mab-hl1626-azide-and-bsa-free-2120249415927.jpg)
![Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Western-Blot-NB300-236-img0001.jpg "Western Blot: Synaptotagmin 1 [p Ser309] Antibody [NB300-236] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Ser309 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.")
![Immunohistochemistry: Synaptotagmin-1 [p Ser309] Antibody [NB300-236] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 in red and PSD95 in green. Photo courtesy of Gang Liu.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Ser309]-Antibody-Immunohistochemistry-NB300-236-img0002.jpg)
![Western Blot: Synaptotagmin 1 Antibody [NBP1-87362] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Western-Blot-NBP1-87362-img0005.jpg "Western Blot: Synaptotagmin 1 Antibody [NBP1-87362] - Analysis in human cerebral cortex tissue.")
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP1-87362] - Analysis in human cerebral cortex and pancreas tissues using NBP1-87362 antibody. Corresponding Synaptotagmin 1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87362-img0007.jpg)
![Western Blot: Synaptotagmin 1 [p Thr202] Antibody [NB300-235] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Thr202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Thr202 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Thr202]-Antibody-Western-Blot-NB300-235-img0001.jpg "Western Blot: Synaptotagmin 1 [p Thr202] Antibody [NB300-235] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Thr202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Thr202 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.")
![Immunohistochemistry: Synaptotagmin-1 [p Thr202] Antibody [NB300-235] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Thr202 in red and PSD95 in green. Photo courtesy of Gang Liu.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Thr202]-Antibody-Immunohistochemistry-NB300-235-img0002.jpg)
![Recombinant Human Synaptotagmin-1 Protein [H00006857-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006857-P01-1.jpg "Recombinant Human Synaptotagmin-1 Protein [H00006857-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Synaptotagmin 1 Antibody [NB300-739] - Western blot of rat cortex showing specific labeling of the ~65k Synaptotagmin protein.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Western-Blot-NB300-739-img0001.jpg "Western Blot: Synaptotagmin 1 Antibody [NB300-739] - Western blot of rat cortex showing specific labeling of the ~65k Synaptotagmin protein.")
![ELISA: Mouse Synaptotagmin 1 ELISA Kit (Colorimetric) [NBP2-80287] - Samples were spiked with high concentrations of Mouse Synaptotagmin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-80287_mouse-synaptotagmin-1-elisa-kit-colorimetric-132202416175696.jpg "ELISA: Mouse Synaptotagmin 1 ELISA Kit (Colorimetric) [NBP2-80287] - Samples were spiked with high concentrations of Mouse Synaptotagmin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Synaptotagmin 1 ELISA Kit (Colorimetric) [NBP2-80287] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-80287_mouse-synaptotagmin-1-elisa-kit-colorimetric-432024955032.jpg)
![Western Blot: Synaptotagmin 1 Overexpression Lysate (Native) [NBP2-09583] - Synaptotagmin 1 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SYT1.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Overexpression-Lysate-Native-Western-Blot-NBP2-09583-img0002.jpg "Western Blot: Synaptotagmin 1 Overexpression Lysate (Native) [NBP2-09583] - Synaptotagmin 1 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SYT1.")