| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33447-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35226-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33528-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22432-50ul | |||
![Western Blot: SNRPA1 Antibody [NBP2-33447] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Western-Blot-NBP2-33447-img0005.jpg "Western Blot: SNRPA1 Antibody [NBP2-33447] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: SNRPA1 Antibody [NBP2-33447] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33447-img0006.jpg)
![Immunohistochemistry-Paraffin: SNRPA1 Antibody [NBP2-33447] - Staining of human stomach, upper shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-33447-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SNRPA1 Antibody [NBP2-33447] - After indicated time periods of SNRPA1 knockdown, cells were collected and analyzed by western blotting. Forty-eight hours after SNRPA1 depletion, protein expression levels of RAD51 and Chk1 were already reduced. At 24h after siSNRPA1 transfection, gamma-H2AX levels transiently increased and then declined. Image collected and cropped by CiteAb from the following publication (nature.com/articles/oncsis201670), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Knockdown-Validated-NBP2-33447-img0008.jpg)
![Immunoprecipitation: SNRPA1 Antibody [NBP2-33447] - Immunoprecipitation of SNRPA1 using NBP2-33447 or Rabbit IgG isotype control at a 1:100 dilution, followed by pulldown with Protein A magnetic beads. IP image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Immunoprecipitation-NBP2-33447-img0009.jpg)
![Western Blot: SNRPA1 Antibody [NBP2-33447] - Recruitment kinetics of SNRPA1 & SF3A3 after laser microirradiation & SNRPA1-dependent recruitment of RAD51 & BRCA1 to laser tracks. (a) Image-based cytometry reveals increased gamma H2AX formation after 72 h SNRPA1 knockdown in U2OS cells. SNRPA1 knockdown effciency is shown in the bar graph. U2OS cells transiently (b) or stably (c) expressing SNRPA1-GFP were microirradiated & protein recruitment followed in real-time. For transcription & splicing inhibition cells were pretreated with DRB (50 μM) or SSA (100 nM) for at least 1 h before damage induction. Representative confocal images & recruitment kinetics are shown. (d) As in (b & c) but in cells stably expressing SF3A3-GFP. (e) Generation of R-loops at laser tracks visualized by HB-GFP recruitment. HB-GFP recruitment kinetics resemble SNRPA1-GFP. (f) Schematic of SNRPA1 deletion constructs. (g) Representative confocal images of recruitment of wild-type & mutated GFP-tagged SNRPA1 after laser microirradiation. (h) Quantification of recruitment, non-recruitment & displacement of GFP-tagged SNRPA1 fusions. Ten cells were analyzed per condition. (i) Confirmation of siRNA-mediated depletion of SNRPA1 after 48 & 72 h knockdown in cells used in (j). (j) Recruitment of RAD51 & BRCA1 in control & SNRPA1-depleted U2OS cells. U2OS cells were transfected with control or SNRPA1 siRNA & 48 or 72 h later microirradiated, fixed after indicated repair times & stained for gamma H2AX, RAD51 & BRCA1. Representative confocal images & percentage of cells displaying RAD51 & BRCA1 recruitment are shown. Scale bar 5 or 10 μM (i). Statistically significant differences were determined using Student's t-test, *P12 foci), gamma H2AX (>12 foci), BRCA1 (>12 foci) & RPA (>12 foci) positive cells. For each condition, more than 400 cells were analyzed. (c) After indicated time periods of SNRPA1 knockdown, cells were collected & analyzed by western blotting. Forty-eight hours after SNRPA1 depletion, protein expression levels of RAD51 & Chk1 were already reduced. At 24 h after siSNRPA1 transfection, gamma H2AX levels transiently increased & then declined. The error bars represent s.e.m. from three independent experiments (n=3). Statistically significant differences between cells treated with control or splicing factor siRNA were determined using Student's t-test, *P<0.05, **P<0.01. Scale bar is 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27991914), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33447_rabbit-polyclonal-snrpa1-antibody-31020241611355.jpg)
![Western Blot: SNRPA1 Antibody (2D7M1) [NBP3-15292] - Western blot analysis of extracts of various cell lines, using SNRPA1 antibody (NBP3-15292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-2D7M1-Western-Blot-NBP3-15292-img0004.jpg "Western Blot: SNRPA1 Antibody (2D7M1) [NBP3-15292] - Western blot analysis of extracts of various cell lines, using SNRPA1 antibody (NBP3-15292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry-Paraffin: SNRPA1 Antibody (2D7M1) [NBP3-15292] - Immunohistochemistry of paraffin-embedded rat testis using SNRPA1 Rabbit mAb (NBP3-15292) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-2D7M1-Immunohistochemistry-Paraffin-NBP3-15292-img0003.jpg)
![Immunohistochemistry-Paraffin: SNRPA1 Antibody (2D7M1) [NBP3-15292] - Immunohistochemistry of paraffin-embedded human breast cancer using SNRPA1 Rabbit mAb (NBP3-15292) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-2D7M1-Immunohistochemistry-Paraffin-NBP3-15292-img0001.jpg)
![Immunohistochemistry-Paraffin: SNRPA1 Antibody (2D7M1) [NBP3-15292] - Immunohistochemistry of paraffin-embedded mouse lung using SNRPA1 Rabbit mAb (NBP3-15292) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-2D7M1-Immunohistochemistry-Paraffin-NBP3-15292-img0002.jpg)
![Immunohistochemistry: SNRPA1 Antibody (2D7M1) [NBP3-15292] - Immunohistochemistry analysis of SNRPA1 in paraffin-embedded human esophagus tissue using SNRPA1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15292_rabbit-snrpa1-mab-2d7m1-72202512443383.jpg)
![Immunohistochemistry: SNRPA1 Antibody (2D7M1) [NBP3-15292] - Immunohistochemistry analysis of SNRPA1 in paraffin-embedded mouse intestin tissue using SNRPA1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15292_rabbit-snrpa1-mab-2d7m1-7220251245381.jpg)
![Immunohistochemistry: SNRPA1 Antibody (2D7M1) [NBP3-15292] - Immunohistochemistry analysis of SNRPA1 in paraffin-embedded human liver tissue using SNRPA1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15292_rabbit-snrpa1-mab-2d7m1-72202512432090.jpg)
![Immunohistochemistry: SNRPA1 Antibody (2D7M1) [NBP3-15292] - Immunohistochemistry analysis of SNRPA1 in paraffin-embedded rat intestine tissue using SNRPA1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15292_rabbit-snrpa1-mab-2d7m1-72202512443319.jpg)
![Immunohistochemistry: SNRPA1 Antibody (2D7M1) [NBP3-15292] - Immunohistochemistry analysis of SNRPA1 in paraffin-embedded human thyroid tissue using SNRPA1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15292_rabbit-snrpa1-mab-2d7m1-72202512435396.jpg)
![Immunohistochemistry: SNRPA1 Antibody [NBP3-35226] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using SNRPA1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35226_rabbit-snrpa1-pab-91220241420138.jpg "Immunohistochemistry: SNRPA1 Antibody [NBP3-35226] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using SNRPA1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: SNRPA1 Antibody [NBP3-35226] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SNRPA1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35226_rabbit-snrpa1-pab-912202414163012.jpg)
![Western Blot: SNRPA1 Antibody [NBP3-35226] - Western blot analysis of various lysates using SNRPA1 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35226_rabbit-snrpa1-pab-91220241519531.jpg)
![Immunohistochemistry: SNRPA1 Antibody [NBP3-35226] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using SNRPA1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35226_rabbit-snrpa1-pab-912202414112821.jpg)
![Immunohistochemistry: SNRPA1 Antibody [NBP3-35226] - Immunohistochemistry analysis of paraffin-embedded Rat liver using SNRPA1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35226_rabbit-snrpa1-pab-91220241515286.jpg)
![Immunohistochemistry: SNRPA1 Antibody [NBP3-35226] - Immunohistochemistry analysis of paraffin-embedded Rat lung using SNRPA1 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35226_rabbit-snrpa1-pab-912202415161830.jpg)
![Immunocytochemistry/Immunofluorescence: SNRPA1 Antibody [NBP2-33528] - Staining of human cell line RT4 shows localization to nucleoplasm & nuclear bodies.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33528-img0002.jpg "Immunocytochemistry/Immunofluorescence: SNRPA1 Antibody [NBP2-33528] - Staining of human cell line RT4 shows localization to nucleoplasm & nuclear bodies.")
![Immunohistochemistry-Paraffin: SNRPA1 Antibody [NBP2-33528] - Staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Immunohistochemistry-Paraffin-NBP2-33528-img0001.jpg)
![Western Blot: SNRPA1 Antibody (SR1474) [NBP3-22432] - Western blot analysis using NBP3-22432 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22432_rabbit-snrpa1-mab-sr1474-57202317161827.jpg "Western Blot: SNRPA1 Antibody (SR1474) [NBP3-22432] - Western blot analysis using NBP3-22432 in HeLa cell lysate.")
![Western Blot: SNRPA1 Antibody [H00006627-B01P] - Analysis of SNRPA1 expression in human liver.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Western-Blot-H00006627-B01P-img0003.jpg "Western Blot: SNRPA1 Antibody [H00006627-B01P] - Analysis of SNRPA1 expression in human liver.")
![Western Blot: SNRPA1 Antibody [H00006627-B01P] - Analysis of SNRPA1 expression in transfected 293T cell line by SNRPA1 polyclonal antibody. Lane 1: SNRPA1 transfected lysate(28.05 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Western-Blot-H00006627-B01P-img0004.jpg)
![Western Blot: SNRPA1 Antibody [NBP1-57240] - Human Liver cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/SNRPA1-Antibody-Western-Blot-NBP1-57240-img0002.jpg "Western Blot: SNRPA1 Antibody [NBP1-57240] - Human Liver cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: SNRPA1 Overexpression Lysate (Adult Normal) [NBL1-16286] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SNRPA1.](http://images.novusbio.com/fullsize/SNRPA1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16286-img0002.jpg "Western Blot: SNRPA1 Overexpression Lysate (Adult Normal) [NBL1-16286] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SNRPA1.")
![SDS-Page: Recombinant Human SNRPA1 Protein [NBP1-78860] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-SNRPA1-Protein-SDS-Page-NBP1-78860-img0001.jpg "SDS-Page: Recombinant Human SNRPA1 Protein [NBP1-78860] - 15% SDS-PAGE (3ug)")