FAB3791P) and either (A) Mouse Anti-Human RORa/NR1F1 Monoclonal Antibody (Catalog # MAB8924) or (B) Mouse IgG
1Isotype Control (
MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (
F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (
FC012)." class="big_lightbox">
FAB3791P) and either (A) Mouse Anti-Human RORa/NR1F1 Monoclonal Antibody (Catalog # MAB8924) or (B) Mouse IgG
1Isotype Control (
MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (
F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (
FC012)." alt="Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (
FAB3791P) and either (A) Mouse Anti-Human RORa/NR1F1 Monoclonal Antibody (Catalog # MAB8924) or (B) Mouse IgG
1Isotype Control (
MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (
F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (
FC012)." class="big_thumb" />
MAB0041, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #
FC012). View our protocol for
Staining Intracellular Molecules." class="big_lightbox">
MAB0041, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #
FC012). View our protocol for
Staining Intracellular Molecules." class="big_thumb" />
FAB3791P) and either (A) Mouse Anti-Human RORa/NR1F1 Monoclonal Antibody (Catalog # MAB8924) or (B) Mouse IgG1Isotype Control (MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012)." alt="Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P) and either (A) Mouse Anti-Human RORa/NR1F1 Monoclonal Antibody (Catalog # MAB8924) or (B) Mouse IgG1Isotype Control (MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012)." class="big_thumb" />
![Western Blot: ROR alpha/NR1F1 Antibody [NBP1-52813] - HepG2 cell lysate, concentration 1.25ug/ml.](http://images.novusbio.com/fullsize/ROR-alpha-NR1F1-Antibody-Western-Blot-NBP1-52813-img0003.jpg)
![Western Blot: ROR alpha/NR1F1 Antibody [NBP1-52813] - BMAL1 controls HIF-1 activity without affecting HIF-alpha -TAD functionA. Evaluation of BMAL1 & ROR alpha expression by Western blot in NP cells stably transduced with lentivirus expressing BMAL1 shRNA. B., C. Densitometric analysis of multiple blots shown in (A) demonstrated decreased BMAL1 (B) & ROR alpha (C) expression in BMAL1-silenced cells under both normoxia & hypoxia. D. HRE activity of BMAL1-silenced NP cells is significantly lower than cells transduced with control shRNA. E.-G. Evaluation of BMAL1 control of HIF-alpha -TAD function. BMAL1 overexpression had no effects on HIF-1 alpha -C-TAD (E), HIF-1 alpha -N-TAD (F), as well as HIF-2 alpha -TAD (G) regardless of oxygen tension. Data is represented as mean ± SE, n= 3, * p<0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27049729), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-52813_rabbit-polyclonal-ror-alpha-nr1f1-antibody-310202415363723.jpg)
![Western Blot: ROR alpha/NR1F1 Antibody [NBP1-52813] - BMAL1 & ROR alpha do not bind to HIF-1 alpha in NP cellsA. Immunoprecipitation (IP) of BMAL1 & CLOCK from NP cells cultured under normoxia or hypoxia for 24 h followed by Western blot analysis using anti-HIF-1 alpha , anti-BMAL1 & anti-CLOCK antibodies. BMAL1 bound to CLOCK, but neither BMAL1 nor CLOCK bound to HIF-1 alpha irrespective of oxygen tension. Preimmune rabbit IgG was used as a negative control for IP assays. B. Pulldown of HIF-1 alpha did not show co-precipitation of BMAL1, CLOCK, or ROR alpha . HC: heavy chain of IgG, nsp: non-specific (C) Pulldown of HIF-1 alpha showed co- precipitation of HIF-1 beta /ARNT, association was higher under hypoxia. D., E. Treatment of NP cells with ROR alpha inhibitor, ML-176 (10 μM), showed no effect on nuclear levels of HIF-1 alpha . Densitometric analysis shown in (E) was performed on blots from 3 independent experiments, Data is represented as mean ± SE, * p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27049729), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-52813_rabbit-polyclonal-ror-alpha-nr1f1-antibody-31020241618946.jpg)
![Western Blot: ROR alpha/NR1F1 Antibody [NBP1-52813] - Expression analysis of BMAL1 & other related factors in NP cellsA. Immunohistochemical localization of BMAL1 in rat intervertebral disc. Sagittal sections of the mature rat intervertebral disc, immunostained with BMAL1 antibody, showed prominent nuclear expression in NP tissue. B. Western blot analysis of BMAL1 & ROR alpha expression in NP tissues isolated from three rats showed positive expression for both the proteins. C. qRT-PCR analysis of BMAL-1 & ROR alpha mRNA expression from NP & AF tissues from rat discs (n=3 animals/group) D. qRT-PCR analysis of BMAL1, ROR alpha , ARNT, ARNT2, ARNTL2 & CLOCK expression in rat NP cells cultured under hypoxia (1% O2). None of the genes showed significant increase in hypoxia. E. Western blot analysis of BMAL1 & ROR alpha in NP cells cultured under hypoxia. F., G. Densitometric analysis of multiple blots shown in (E) above. No significant differences were seen between normoxic & hypoxic levels of BMAL1 & ROR alpha . Data is represented as mean ± SE, n=3, p<0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27049729), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-52813_rabbit-polyclonal-ror-alpha-nr1f1-antibody-3102024161601.jpg)
MAB0041, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ![Immunohistochemistry-Paraffin: ROR alpha/NR1F1 Antibody [NLS2337] - Human, Skin: Formalin-Fixed Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/ROR-alpha-NR1F1-Antibody-Immunohistochemistry-Paraffin-NLS2337-img0002.jpg "Immunohistochemistry-Paraffin: ROR alpha/NR1F1 Antibody [NLS2337] - Human, Skin: Formalin-Fixed Paraffin-Embedded (FFPE)")
![Immunohistochemistry-Paraffin: ROR alpha/NR1F1 Antibody [NLS2337] - Human, Brain, Cortex: Formalin-Fixed Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/ROR-alpha-NR1F1-Antibody-Immunohistochemistry-Paraffin-NLS2337-img0001.jpg)
![Western Blot: ROR alpha/NR1F1 Antibody [NBP1-52828] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%](http://images.novusbio.com/fullsize/ROR-alpha-NR1F1-Antibody-Western-Blot-NBP1-52828-img0006.jpg "Western Blot: ROR alpha/NR1F1 Antibody [NBP1-52828] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%")
![Immunohistochemistry-Paraffin: ROR alpha/NR1F1 Antibody [NBP1-52828] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/ROR-alpha-NR1F1-Antibody-Immunohistochemistry-Paraffin-NBP1-52828-img0003.jpg)
![Western Blot: ROR alpha/NR1F1 Antibody [NBP1-52828] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml](http://images.novusbio.com/fullsize/ROR-alpha-NR1F1-Antibody-Western-Blot-NBP1-52828-img0002.jpg)
![Western Blot: ROR alpha Overexpression Lysate (Adult Normal) [NBL1-15480] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ROR alpha.](http://images.novusbio.com/fullsize/ROR-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15480-img0002.jpg "Western Blot: ROR alpha Overexpression Lysate (Adult Normal) [NBL1-15480] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ROR alpha.")
![Western Blot: ROR alpha Overexpression Lysate (Adult Normal) [NBP2-05658] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ROR alpha.](http://images.novusbio.com/fullsize/ROR-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05658-img0001.jpg "Western Blot: ROR alpha Overexpression Lysate (Adult Normal) [NBP2-05658] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ROR alpha.")
![Western Blot: ROR alpha Overexpression Lysate (Adult Normal) [NBL1-15479] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ROR alpha.](http://images.novusbio.com/fullsize/ROR-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15479-img0002.jpg "Western Blot: ROR alpha Overexpression Lysate (Adult Normal) [NBL1-15479] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ROR alpha.")
