| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87791-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF16041-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1604-SP | |||
HAF007). For additional reference, recombinant human MKK3 and MKK6 (2 ng/lane) were included. Specific bands for MKK3 and MKK6 was detected at approximately 40 kDa and 38 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows lysates of NRK rat normal kidney cell and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat MKK3/MKK6 Monoclonal Antibody (Catalog # MAB2514) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MKK3 and MKK6 (2 ng/lane) were included. Specific bands for MKK3 and MKK6 was detected at approximately 40 kDa and 38 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2514-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21693-50ul | |||
![Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining in human colon and testis tissues using anti-MAP2K6 antibody. Corresponding MAP2K6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-Immunohistochemistry-Paraffin-NBP1-87791-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining in human colon and testis tissues using anti-MAP2K6 antibody. Corresponding MAP2K6 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: MKK6/MEK6 Antibody [NBP1-87791] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-Western-Blot-NBP1-87791-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MKK6/MEK6 Antibody [NBP1-87791] - Staining of human cell line A549 shows localization to nucleus & cytosol.](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87791-img0006.jpg)
![Western Blot: MKK6/MEK6 Antibody [NBP1-87791] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-341](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-Western-Blot-NBP1-87791-img0004.jpg)
![Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining of human colon shows high expression.](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-Immunohistochemistry-Paraffin-NBP1-87791-img0008.jpg)
![Immunohistochemistry-Paraffin: MKK6/MEK6 Antibody [NBP1-87791] - Staining of human testis shows low expression as expected.](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-Immunohistochemistry-Paraffin-NBP1-87791-img0009.jpg)
![Immunohistochemistry: MKK3/MKK6 Antibody (SR1645) [NBP3-21693] - Immunohistochemical analysis of paraffin-embedded mouse spleen, using NBP3-21693.](http://images.novusbio.com/fullsize/nbp3-21693_rabbit-mkk3-mkk6-mab-sr1645-57202315521824.jpg "Immunohistochemistry: MKK3/MKK6 Antibody (SR1645) [NBP3-21693] - Immunohistochemical analysis of paraffin-embedded mouse spleen, using NBP3-21693.")
![Immunocytochemistry/Immunofluorescence: MKK3/MKK6 Antibody (SR1645) [NBP3-21693] - Immunofluorescent analysis of Hela cells, using NBP3-21693.](http://images.novusbio.com/fullsize/nbp3-21693_rabbit-mkk3-mkk6-mab-sr1645-5720231721515.jpg)
![Western Blot: MKK3/MKK6 Antibody (SR1645) [NBP3-21693] - Western blot analysis using NBP3-21693 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21693_rabbit-mkk3-mkk6-mab-sr1645-57202316494280.jpg)
![Western Blot: MKK6/MEK6 [p Ser207] Antibody [NB100-82050] - Analysis of extracts from HL60 cells untreated or treated with PMA using MKK6(Phospho-Ser207) antibody](http://images.novusbio.com/fullsize/MKK6-MEK6-[p-Ser207]-Antibody-Western-Blot-NB100-82050-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: MKK6/MEK6 [p Ser207] Antibody [NB100-82050] - Analysis of extracts from HL60 cells untreated or treated with PMA using MKK6(Phospho-Ser207) antibody")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MKK6/MEK6 [p Ser207] Antibody [NB100-82050] - Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using NB100-82050(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-82050_rabbit-polyclonal-mkk6-mek6-p-ser207-antibody-103202523464556.jpg)
![Immunocytochemistry/Immunofluorescence: MKK6/MEK6 [p Ser207] Antibody [NB100-82050] - Staining of methanol-fixed Hela cells showing cytoplasmic staining using NB100-82050](http://images.novusbio.com/fullsize/nb100-82050_rabbit-polyclonal-mkk6-mek6-p-ser207-antibody-1132025155591.jpg)
![Western Blot: MKK6/MEK6 Antibody (2F2) [H00005608-M02] - MAP2K6 monoclonal antibody (M02), clone 2F2 Analysis of MAP2K6 expression in COLO 320 HSR.](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-2F2-Western-Blot-H00005608-M02-img0009.jpg "Western Blot: MKK6/MEK6 Antibody (2F2) [H00005608-M02] - MAP2K6 monoclonal antibody (M02), clone 2F2 Analysis of MAP2K6 expression in COLO 320 HSR.")
![Immunocytochemistry/Immunofluorescence: MKK6/MEK6 Antibody (2F2) [H00005608-M02] - Analysis of monoclonal antibody to MAP2K6 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-2F2-Immunocytochemistry-Immunofluorescence-H00005608-M02-img0007.jpg)
![Proximity Ligation Assay: MKK6/MEK6 Antibody (2F2) [H00005608-M02] - Analysis of protein-protein interactions between MAP2K3 and MAP2K6. HeLa cells were stained with anti-MAP2K3 rabbit purified polyclonal 1:1200 and anti-MAP2K6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-2F2-Proximity-Ligation-Assay-H00005608-M02-img0005.jpg)
![Sandwich ELISA: MKK6/MEK6 Antibody (2F2) [H00005608-M02] - Detection limit for recombinant GST tagged MAP2K6 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MKK6-MEK6-Antibody-2F2-Sandwich-ELISA-H00005608-M02-img0008.jpg)
![Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946] - Western blot analysis of extracts of various cell lines, using MKK3/MKK6 Rabbit mAb (NBP3-15946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MKK3-MKK6-Antibody-8R1J5-Western-Blot-NBP3-15946-img0001.jpg "Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946] - Western blot analysis of extracts of various cell lines, using MKK3/MKK6 Rabbit mAb (NBP3-15946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946] - Western blot analysis of extracts of various cell lines, using MKK3/MKK6 Rabbit mAb (NBP3-15946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/MKK3-MKK6-Antibody-8R1J5-Western-Blot-NBP3-15946-img0002.jpg)
![Western Blot: MEK6 Overexpression Lysate (Adult Normal) [NBL1-12851] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MEK6.](http://images.novusbio.com/fullsize/MEK6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12851-img0002.jpg "Western Blot: MEK6 Overexpression Lysate (Adult Normal) [NBL1-12851] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MEK6.")
![SDS-Page: Recombinant Human MKK6/MEK6 His Protein [NBP2-51949] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Human-MKK6-MEK6-His-Protein-SDS-Page-NBP2-51949-img0002.jpg "SDS-Page: Recombinant Human MKK6/MEK6 His Protein [NBP2-51949] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![SDS-Page: Recombinant Human MKK6/MEK6 His Protein [NBP3-11963] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant-Human-MKK6-MEK6-His-Protein-SDS-Page-NBP3-11963-img0001.jpg "SDS-Page: Recombinant Human MKK6/MEK6 His Protein [NBP3-11963] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain")
