HAF109). A specific band was detected for PKC gamma at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKC gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5130) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PKC gamma at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5130-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38728-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38569-25ul | |||
![Western Blot: PKC gamma Antibody [NBP1-32535] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Western-Blot-NBP1-32535-img0007.jpg "Western Blot: PKC gamma Antibody [NBP1-32535] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![PKC gamma antibody detects PKC gamma protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: PKC gamma protein stained by PKC gamma antibody (NBP1-32535) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-32535_rabbit-polyclonal-pkc-gamma-antibody-immunohistochemistry-paraffin-141120251840542.jpg)
![Immunohistochemistry-Paraffin: PKC gamma Antibody [NBP1-32535] - Paraffin-embedded Hun7 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Immunohistochemistry-Paraffin-NBP1-32535-img0002.jpg)
![Immunohistochemistry-Paraffin: PKC gamma Antibody [NBP1-32535] - Paraffin-embedded rat brain. PKC gamma antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Immunohistochemistry-Paraffin-NBP1-32535-img0004.jpg)
![Immunohistochemistry-Paraffin: PKC gamma Antibody [NBP1-32535] - Paraffin-embedded mouse brain. PKC gamma antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Immunohistochemistry-Paraffin-NBP1-32535-img0005.jpg)
![Western Blot: PKC gamma Antibody [NBP1-32535] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody (NBP1-32535) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32535_rabbit-polyclonal-pkc-gamma-antibody-91020242015722.jpg)
![Western Blot: PKC gamma Antibody [NBP1-32535] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody (NBP1-32535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32535_rabbit-polyclonal-pkc-gamma-antibody-91020242001029.jpg)
![Western Blot: PKC gamma Antibody [NBP1-32535] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody (NBP1-32535) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32535_rabbit-polyclonal-pkc-gamma-antibody-910202420115010.jpg)
![Western Blot: PKC gamma Antibody [NBP1-58916] - Human neuroblastoma cells untreated or treated with various drugs. Image from verified customer review.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Western-Blot-NBP1-58916-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: PKC gamma Antibody [NBP1-58916] - Human neuroblastoma cells untreated or treated with various drugs. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: PKC gamma Antibody [NBP1-58916] - Quiescent human colon carcinoma cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP1-58916-img0005.jpg)
![Western Blot: PKC gamma Antibody [NBP1-58916] - Human Placenta lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Western-Blot-NBP1-58916-img0002.jpg)
![Western Blot: PKC gamma Antibody (HL2226) - Azide and BSA Free [NBP3-25606] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2226] (NBP3-25606) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25606_rabbit-pkc-gamma-mab-hl2226-azide-and-bsa-free-2120249371514.jpg "Western Blot: PKC gamma Antibody (HL2226) - Azide and BSA Free [NBP3-25606] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2226] (NBP3-25606) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: PKC gamma Antibody (HL2226) - Azide and BSA Free [NBP3-25606] - PKC proteins (10 ng) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2226] (NBP3-25606) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25606_rabbit-pkc-gamma-mab-hl2226-azide-and-bsa-free-2120249432884.jpg)
![Immunohistochemistry-Paraffin: PKC gamma Antibody (HL2226) - Azide and BSA Free [NBP3-25606] - PKC gamma antibody [HL2226] detects PKC gamma protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PKC gamma stained by PKC gamma antibody [HL2226] (NBP3-25606) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25606_rabbit-pkc-gamma-mab-hl2226-azide-and-bsa-free-212024941435.jpg)
![Western Blot: PKC gamma Antibody (HL2226) - Azide and BSA Free [NBP3-25606] - Human brain (5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC gamma antibody [HL2226] (NBP3-25606) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25606_rabbit-pkc-gamma-mab-hl2226-azide-and-bsa-free-212024937154.jpg)
![Immunohistochemistry-Paraffin: PKC gamma Antibody (HL2226) - Azide and BSA Free [NBP3-25606] - PKC gamma antibody [HL2226] detects PKC gamma protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. PKC gamma stained by PKC gamma antibody [HL2226] (NBP3-25606) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25606_rabbit-pkc-gamma-mab-hl2226-azide-and-bsa-free-2120249371578.jpg)
![Western Blot: PKC gamma Antibody (A12-H) [NBP1-30124] - Left Lane: Marker, Right Lane: Mouse brain crude lysate (200ug of protein loaded).](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-A12-H-Western-Blot-NBP1-30124-img0001.jpg "Western Blot: PKC gamma Antibody (A12-H) [NBP1-30124] - Left Lane: Marker, Right Lane: Mouse brain crude lysate (200ug of protein loaded).")
![Immunocytochemistry/Immunofluorescence: PKC gamma Antibody (A12-H) [NBP1-30124] - PKC-gamma expression in HEK293 cells.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-A12-H-Immunocytochemistry-Immunofluorescence-NBP1-30124-img0003.jpg)
![Immunohistochemistry: PKC gamma Antibody - BSA Free [NBP3-48269] - Immunohistochemistry of paraffin-embedded rat brain tissue slide using (PKC gamma Antibody) at dilution of 1:200](http://images.novusbio.com/fullsize/nbp3-48269_rabbit-pkc-gamma-pab-bsa-free-28420251304188.jpg "Immunohistochemistry: PKC gamma Antibody - BSA Free [NBP3-48269] - Immunohistochemistry of paraffin-embedded rat brain tissue slide using (PKC gamma Antibody) at dilution of 1:200")
![Western Blot: PKC gamma Antibody - BSA Free [NBP3-48269] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (PKC gamma antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-48269_rabbit-pkc-gamma-pab-bsa-free-284202512582687.jpg)
![Immunohistochemistry-Paraffin: PKC gamma Antibody [NBP2-38728] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-38728-img0003.jpg "Immunohistochemistry-Paraffin: PKC gamma Antibody [NBP2-38728] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.")
![Immunohistochemistry: PKC gamma Antibody [NBP2-38569] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Immunohistochemistry-NBP2-38569-img0001.jpg "Immunohistochemistry: PKC gamma Antibody [NBP2-38569] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.")
![Western Blot: PKC gamma Antibody (5F2X3) [NBP3-16611] - Western blot analysis of extracts of various cell lines, using PKC gamma Rabbit mAb (NBP3-16611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-5F2X3-Western-Blot-NBP3-16611-img0001.jpg "Western Blot: PKC gamma Antibody (5F2X3) [NBP3-16611] - Western blot analysis of extracts of various cell lines, using PKC gamma Rabbit mAb (NBP3-16611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: PKC gamma Antibody [NBP1-58917] - 293T cells lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-Western-Blot-NBP1-58917-img0002.jpg "Western Blot: PKC gamma Antibody [NBP1-58917] - 293T cells lysate, concentration 0.2-1 ug/ml.")
![Western Blot: PKC gamma Overexpression Lysate (Adult Normal) [NBL1-14775] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PKC gamma.](http://images.novusbio.com/fullsize/PKC-gamma-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14775-img0002.jpg "Western Blot: PKC gamma Overexpression Lysate (Adult Normal) [NBL1-14775] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PKC gamma.")
