Hu,
Mu,
Rt,
ChHaSpecies Glossary
Reactivity: Hu, Mu, Rt, ChHa
WB, ICC/IF, IHC, IHC-P, PEP-ELISA, ICC
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA, ICC
Host: Goat Polyclonal
Alt Catalog# IMG-3575
Sample Size Available
Sizes: Sample Size Available
![Western Blot: VPS35 Antibody [NB100-1397] - Staining of Human Brain (Cerebellum) (A) and Rat Brain (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NB100-1397-img0016.jpg "Western Blot: VPS35 Antibody [NB100-1397] - Staining of Human Brain (Cerebellum) (A) and Rat Brain (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence")
![Immunocytochemistry: VPS35 Antibody [NB100-1397] - Staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunocytochemistry-NB100-1397-img0010.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-Paraffin-NB100-1397-img0018.jpg)
![Immunocytochemistry: VPS35 Antibody [NB100-1397] - Staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunocytochemistry-NB100-1397-img0009.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-Paraffin-NB100-1397-img0017.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - A. 50 ug rat brain lysate/extract 7.5% SDS-PAGE VPS35 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NBP1-31069-img0016.jpg "Western Blot: VPS35 Antibody [NBP1-31069] - A. 50 ug rat brain lysate/extract 7.5% SDS-PAGE VPS35 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NBP1-31069] - VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS35 protein stained by VPS35 antibody [C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31069-img0011.jpg)
![Immunohistochemistry: VPS35 Antibody [NBP1-31069] - VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term dilution: 1:250.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-NBP1-31069-img0006.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/VPS35 Antibody-Western Blot-NBP1-31069-img0004.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - Sample(30 ug of whole cell lysate)A:H1299B:HeLa S3 C:Hep G2 D:MOLT4 E:Raji 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/VPS35 Antibody-Western Blot-NBP1-31069-img0005.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - A. 30 ug A549 whole cell extract B. 30 ug H1299 whole cell extract C. 30 ug HCT116 whole cell extract 7.5% SDS-PAGE VPS35 antibody [C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NBP1-31069-img0013.jpg)
![Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NBP2-13517-img0007.jpg "Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NBP2-13517] - Staining of human cell line U-2 OS shows localization to endosomes & lysosomes.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13517-img0008.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP2-13517-img0009.jpg)
![Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NBP2-13517-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NBP2-13517] - Staining of human cell line U-2 OS shows positivity in vesicles.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13517-img0003.jpg)
![Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in HeLa.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(2D3)-Western-Blot-H00055737-M02-img0001.jpg "Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in HeLa.")
![Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in human colon.](http://images.novusbio.com/fullsize2/VPS35 Antibody (2D3)-Western Blot-H00055737-M02-img0002.jpg)
![Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/VPS35 Antibody-Western Blot-NBP2-20876-img0002.jpg "Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-20876] - Immunohistochemical analysis of paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/VPS35 Antibody-Immunohistochemistry-Paraffin-NBP2-20876-img0003.jpg)
![Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/VPS35 Antibody-Western Blot-NBP2-20876-img0001.jpg)
![Immunoprecipitation: VPS35 Antibody [NBP2-20876] - Immunoprecipitation of VPS35 protein from HeLa whole cell extracts using 5 ug of VPS35 antibody. Western blot analysis was performed using VPS35 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunoprecipitation-NBP2-20876-img0006.jpg)
![SDS-Page: Recombinant Human VPS35 Protein [H00055737-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize2/Recombinant-Human-VPS35-Protein-SDS-Page-H00055737-P01-img0001.jpg "SDS-Page: Recombinant Human VPS35 Protein [H00055737-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: VPS35 Overexpression Lysate (Adult Normal) [NBL1-17746] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VPS35.](http://images.novusbio.com/fullsize/VPS35 Overexpression Lysate (Adult Normal)-Western Blot-NBL1-17746-img0002.jpg "Western Blot: VPS35 Overexpression Lysate (Adult Normal) [NBL1-17746] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VPS35.")