Hu,
Mu,
Rt,
Ca,
Ch,
ChHa,
BvSpecies Glossary
Reactivity: Hu, Mu, Rt, Ca, Ch, ChHa, Bv
WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Host: Goat Polyclonal
Alt Catalog# IMG-3575
Sample Size Available
Sizes: Sample Size Available
![Western Blot: VPS35 Antibody [NB100-1397] - Staining of HepG2 (A) and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NB100-1397-img0009.jpg "Western Blot: VPS35 Antibody [NB100-1397] - Staining of HepG2 (A) and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NB100-1397] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1397-img0011.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Negative Control showing staining of paraffin embedded Human Prostate, with no primary antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-Paraffin-NB100-1397-img0005.jpg)
![Western Blot: VPS35 Antibody [NB100-1397] - 10 ug cell lysate per lane of various cell lines as indicated, immunoblotted with vps35 antibody at 1:2000 at 4C for 20 hrs. Secondary antibody donkey anti-goat HRP at 1:5000 for 1 hr at room temp. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NB100-1397-img0006.jpg)
![Western Blot: VPS35 Antibody [NB100-1397] - HEK293T cell lysate. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NB100-1397-img0007.jpg)
![Western Blot: VPS35 Antibody [NB100-1397] - Staining of Human (A) Mouse (B) and Rat (C) Brain lysate (35 ug protein in RIPA buffer). antibody at 0.03 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NB100-1397-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NB100-1397] - Confocal microscopy image of human RPE-1 cells fixed with PFA, permeablilized with 0.3% Triton X-100,then immunostained with vps35 antibody at 1:2000 overnight in 1% BSA at 4 degrees. ICC image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunocytochemistry-NB100-1397-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NB100-1397] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1397-img0010.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NB100-1397] - Staining of paraffin embedded Human Prostate. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-Paraffin-NB100-1397-img0004.jpg)
![Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VPS35 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NBP2-13517-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-VPS35 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NBP2-13517] - Staining of human cell line U-2 OS shows localization to endosomes & lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13517-img0008.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining in human skin and skeletal muscle tissues . Corresponding VPS35 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP2-13517-img0016.jpg)
![Western Blot: VPS35 Antibody [NBP2-13517] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NBP2-13517-img0007.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining of human skin shows moderate cytoplasmic positivity in keratinocytes.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP2-13517-img0010.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP2-13517-img0011.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining of human testis shows moderate cytoplasmic and membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP2-13517-img0012.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining of human skeletal muscle shows very weak positivity in myocytes.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP2-13517-img0014.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-13517] - Staining of human substantia nigra shows moderate cytoplasmic positivity in neurons and fibers.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP2-13517-img0015.jpg)
![Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in human colon.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(2D3)-Western-Blot-H00055737-M02-img0002.jpg "Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in human colon.")
![Western Blot: VPS35 Antibody (2D3) [H00055737-M02] - VPS35 monoclonal antibody (M02), clone 2D3. Analysis of VPS35 expression in HeLa.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(2D3)-Western-Blot-H00055737-M02-img0001.jpg)
![ELISA: VPS35 Antibody (2D3) [H00055737-M02] - Detection limit for recombinant GST tagged VPS35 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(2D3)-ELISA-H00055737-M02-img0003.jpg)
![Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(7E4)-Western-Blot-NBP2-78823-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: VPS35 Antibody (7E4) [NBP2-78823] - Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(7E4)-Immunocytochemistry-Immunofluorescence-NBP2-78823-img0001.jpg)
![Western Blot: VPS35 Antibody (7E4) [NBP2-78823] - Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 91.7 kDa.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(7E4)-Western-Blot-NBP2-78823-img0006.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823). 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4C. NBP2-78823 clone 7E4 depletes virtually all of the VPS35 from the A549 cell extract..](http://images.novusbio.com/fullsize2/VPS35-Antibody-(7E4)-Immunoprecipitation-NBP2-78823-img0002.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 7E4 (NBP2-78823). Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823).](http://images.novusbio.com/fullsize2/VPS35-Antibody-(7E4)-Immunoprecipitation-NBP2-78823-img0003.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823). Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of A549 lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(7E4)-Immunoprecipitation-NBP2-78823-img0004.jpg)
![Immunoprecipitation: VPS35 Antibody (7E4) [NBP2-78823] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78823). Three amounts of NBP2-78823 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(7E4)-Immunoprecipitation-NBP2-78823-img0005.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NBP1-31069-img0018.jpg "Western Blot: VPS35 Antibody [NBP1-31069] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: VPS35 Antibody [NBP1-31069] - VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS35 protein stained by VPS35 antibody [C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31069-img0011.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP1-31069] - VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term dilution: 1:250.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunohistochemistry-Paraffin-NBP1-31069-img0017.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/VPS35 Antibody-Western Blot-NBP1-31069-img0004.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - Sample(30 ug of whole cell lysate)A:H1299B:HeLa S3 C:Hep G2 D:MOLT4 E:Raji 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/VPS35 Antibody-Western Blot-NBP1-31069-img0005.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - A. 30 ug A549 whole cell extract B. 30 ug H1299 whole cell extract C. 30 ug HCT116 whole cell extract 7.5% SDS-PAGE VPS35 antibody [C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NBP1-31069-img0013.jpg)
![Western Blot: VPS35 Antibody [NBP1-31069] - A. 50 ug rat brain lysate/extract 7.5% SDS-PAGE VPS35 antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NBP1-31069-img0016.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(8A3)-Western-Blot-NBP2-78825-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: VPS35 Antibody (8A3) [NBP2-78825] - Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells. Courtesy of: Dario Alessi Lab, University of Dundee.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(8A3)-Immunocytochemistry-Immunofluorescence-NBP2-78825-img0007.jpg)
![Western Blot: VPS35 Antibody (8A3) [NBP2-78825] - Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(8A3)-Western-Blot-NBP2-78825-img0003.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825). Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(8A3)-Immunoprecipitation-NBP2-78825-img0001.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825). Three amounts of NBP2-78825 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of A549 lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(8A3)-Immunoprecipitation-NBP2-78825-img0002.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3 (NBP2-78825). Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825).](http://images.novusbio.com/fullsize2/VPS35-Antibody-(8A3)-Immunoprecipitation-NBP2-78825-img0005.jpg)
![Immunoprecipitation: VPS35 Antibody (8A3) [NBP2-78825] - Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78825). 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degrees C. NBP2-78825 clone 8A3 depletes VPS35 from the A549 cell extract..](http://images.novusbio.com/fullsize2/VPS35-Antibody-(8A3)-Immunoprecipitation-NBP2-78825-img0006.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(HL1017)-Western-Blot-NBP3-13680-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody detects VPS35 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS35 stained by VPS35 antibody (NBP3-13680) diluted at 1:500.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(HL1017)-Immunocytochemistry-Immunofluorescence-NBP3-13680-img0004.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (HL1017) [NBP3-13680] - VPS35 antibody [HL1017] detects VPS35 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. VPS35 stained by VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize2/VPS35-Antibody-(HL1017)-Immunohistochemistry-Paraffin-NBP3-13680-img0005.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(HL1017)-Western-Blot-NBP3-13680-img0001.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(HL1017)-Western-Blot-NBP3-13680-img0002.jpg)
![Western Blot: VPS35 Antibody (HL1017) [NBP3-13680] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (NBP3-13680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(HL1017)-Western-Blot-NBP3-13680-img0003.jpg)
![Western Blot: VPS35 Antibody [NBP2-20876] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Western-Blot-NBP2-20876-img0007.jpg "Western Blot: VPS35 Antibody [NBP2-20876] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: VPS35 Antibody [NBP2-20876] - A549 xenograft, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize2/VPS35 Antibody-Immunohistochemistry-Paraffin-NBP2-20876-img0003.jpg)
![Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/VPS35 Antibody-Western Blot-NBP2-20876-img0001.jpg)
![Western Blot: VPS35 Antibody [NBP2-20876] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/VPS35 Antibody-Western Blot-NBP2-20876-img0002.jpg)
![Immunoprecipitation: VPS35 Antibody [NBP2-20876] - Immunoprecipitation of VPS35 protein from HeLa whole cell extracts using 5 ug of VPS35 antibody. Western blot analysis was performed using VPS35 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize2/VPS35-Antibody-Immunoprecipitation-NBP2-20876-img0006.jpg)
![Western Blot: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution.Positive control:Lane 1: PC-3M Lane 2: U937Lane 3: SH-SY-5Y](http://images.novusbio.com/fullsize2/VPS35-Antibody-(JB33-82)-Western-Blot-NBP2-75710-img0006.jpg "Western Blot: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of VPS35 on different lysates using anti-VPS35 antibody at 1/500 dilution.Positive control:Lane 1: PC-3M Lane 2: U937Lane 3: SH-SY-5Y")
![Immunohistochemistry-Paraffin: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of paraffin-embedded mouse colon tissue using anti-VPS35 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(JB33-82)-Immunohistochemistry-Paraffin-NBP2-75710-img0005.jpg)
![Flow Cytometry: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of 293T cells with VPS35 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(JB33-82)-Flow-Cytometry-NBP2-75710-img0001.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of paraffin-embedded human colon cancer tissue using anti-VPS35 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(JB33-82)-Immunohistochemistry-Paraffin-NBP2-75710-img0002.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of paraffin-embedded human kidney tissue using anti-VPS35 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(JB33-82)-Immunohistochemistry-Paraffin-NBP2-75710-img0003.jpg)
![Immunohistochemistry-Paraffin: VPS35 Antibody (JB33-82) [NBP2-75710] - Analysis of paraffin-embedded human placenta tissue using anti-VPS35 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(JB33-82)-Immunohistochemistry-Paraffin-NBP2-75710-img0004.jpg)
![Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(11H10)-Western-Blot-NBP2-78827-img0004.jpg "Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.")
![<b>Genetic Strategies Validation. </b>Western Blot: VPS35 Antibody (11H10) [NBP2-78827] - Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 ug each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827) at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(11H10)-Western-Blot-NBP2-78827-img0003.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827). 500 uL cell culture supernatants were incubated with 10 uL of Protein A/G resin beads for 1 hour at 4 degrees C. NBP2-78827 clone 11H10 depletes VPS35 from the A549 cell extract.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(11H10)-Immunoprecipitation-NBP2-78827-img0001.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Co-Immunoprecipitation analysis of known interactors of VPS35 using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10 (NBP2-78827). Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827).](http://images.novusbio.com/fullsize2/VPS35-Antibody-(11H10)-Immunoprecipitation-NBP2-78827-img0002.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827). Three amounts of NBP2-78827 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of A549 lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(11H10)-Immunoprecipitation-NBP2-78827-img0005.jpg)
![Immunoprecipitation: VPS35 Antibody (11H10) [NBP2-78827] - Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody (NBP2-78827). Three amounts of NBP2-78827 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degrees C and next incubated with 250ug of MEF lysate for 2 hours at 4 degrees C.](http://images.novusbio.com/fullsize2/VPS35-Antibody-(11H10)-Immunoprecipitation-NBP2-78827-img0006.jpg)
![SDS-Page: Recombinant Human VPS35 Protein [H00055737-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize2/Recombinant-Human-VPS35-Protein-SDS-Page-H00055737-P01-img0001.jpg "SDS-Page: Recombinant Human VPS35 Protein [H00055737-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: VPS35 Antibody (8D3L3) [NBP3-16803] - Western blot analysis of extracts of various cell lines, using VPS35 Rabbit mAb (NBP3-16803) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize2/VPS35 Antibody (8D3L3)-Western Blot-NBP3-16803-img0001.jpg "Western Blot: VPS35 Antibody (8D3L3) [NBP3-16803] - Western blot analysis of extracts of various cell lines, using VPS35 Rabbit mAb (NBP3-16803) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")