HAF016). A specific band was detected for Girdin at approximately 200 to 220 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." alt="Western blot shows lysates of H69AR human small cell lung cancer cell line, MRC-5 human embryonic lung fibroblast cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Girdin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5345) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Girdin at approximately 200 to 220 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5345-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76253-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38387-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90135-25ul | |||
HAF016). This experiment was conducted under reducing conditions and using the 66-440kDa separation system." class="big_thumb" />
![Western Blot: Girdin Antibody [NBP1-76253] - Rat brain tissue lysate with Girdin antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/Girdin-Antibody-Western-Blot-NBP1-76253-img0001.jpg "Western Blot: Girdin Antibody [NBP1-76253] - Rat brain tissue lysate with Girdin antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: Girdin Antibody - BSA Free [NBP1-76253] - Immunofluorescence of Girdin in human brain tissue with Girdin antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76253_rabbit-polyclonal-girdin-antibody-2711202419514043.jpg)
![Western Blot: Girdin Antibody (M012) [NBP3-23351] - Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23351_mouse-girdin-mab-m012-199202318202922.jpg "<b>Biological Strategies Validation. </b>Western Blot: Girdin Antibody (M012) [NBP3-23351] - Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Girdin Antibody (M012) [NBP3-23351] - Immunocytochemical labeling of Girdin in aldehyde fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Girdin (NBP3-23351). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23351_mouse-girdin-mab-m012-1992023199284.jpg)
![Immunohistochemistry-Paraffin: Girdin Antibody [NBP2-38387] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Girdin-Antibody-Immunohistochemistry-Paraffin-NBP2-38387-img0003.jpg "Immunohistochemistry-Paraffin: Girdin Antibody [NBP2-38387] - Staining of human testis shows high expression.")
![Immunohistochemistry-Paraffin: Girdin Antibody [NBP2-38387] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Girdin-Antibody-Immunohistochemistry-Paraffin-NBP2-38387-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Girdin Antibody [NBP2-38387] - Staining in human testis and pancreas tissues using anti-CCDC88A antibody. Corresponding CCDC88A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Girdin-Antibody-Immunohistochemistry-Paraffin-NBP2-38387-img0004.jpg)
![Western Blot: Girdin Antibody [NBP1-71857] - Detection of Human CCDC88A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC88A antibody NBP1-71857 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Girdin-Antibody-Western-Blot-NBP1-71857-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Girdin Antibody [NBP1-71857] - Detection of Human CCDC88A by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC88A antibody NBP1-71857 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Girdin Antibody [NBP1-71857] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CCDC88A antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCDC88A was also immunoprecipitated by rabbit anti-CCDC88A antibody NBP1-71856 , which recognizes an upstream epitope. For blotting immunoprecipitated CCDC88A, NBP1-71857 was used at 1 ug/ml.](http://images.novusbio.com/fullsize/Girdin-Antibody-Western-Blot-NBP1-71857-img0001.jpg)
![Western Blot: Girdin [p Ser1674] Antibody [NBP3-23205] - Western blot image of human A431 cell lysates treated with Calyculin A (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Ser-1674), phospho-specific (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23205_rabbit-girdin-p-ser1674-pab-199202317594420.jpg "<b>Biological Strategies Validation. </b>Western Blot: Girdin [p Ser1674] Antibody [NBP3-23205] - Western blot image of human A431 cell lysates treated with Calyculin A (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Ser-1674), phospho-specific (lanes 3 & 4).")
![Western Blot: Girdin [p Ser1416] Antibody [NBP3-23336] - Western blot image of human A431 cell lysates treated with Calyculin A (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Ser-1416), phospho-specific (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23336_rabbit-girdin-p-ser1416-pab-19920231820298.jpg "<b>Biological Strategies Validation. </b>Western Blot: Girdin [p Ser1416] Antibody [NBP3-23336] - Western blot image of human A431 cell lysates treated with Calyculin A (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Ser-1416), phospho-specific (lanes 3 & 4).")
![Western Blot: Girdin [p Tyr1764] Antibody [NBP3-23352] - Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23352_rabbit-girdin-p-tyr1764-pab-19920231856955.jpg "<b>Biological Strategies Validation. </b>Western Blot: Girdin [p Tyr1764] Antibody [NBP3-23352] - Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).")
![Immunoprecipitation: Girdin Antibody [NBP1-71856] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CCDC88A antibody used for IP at 6 mcg/mg lysate. CCDC88A was also immunoprecipitated by rabbit anti-CCDC88A antibody NBP1-71857 , which recognizes a downstream epitope. For blotting immunoprecipitated CCDC88A, NBP1-71857 was used at 1 ug/ml.](http://images.novusbio.com/fullsize/Girdin-Antibody-Western-Blot-NBP1-71856-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: Girdin Antibody [NBP1-71856] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-CCDC88A antibody used for IP at 6 mcg/mg lysate. CCDC88A was also immunoprecipitated by rabbit anti-CCDC88A antibody NBP1-71857 , which recognizes a downstream epitope. For blotting immunoprecipitated CCDC88A, NBP1-71857 was used at 1 ug/ml.")
