| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43955 | |||
| BSA Free | NBP2-30673-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21933-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55731-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90016-25ul | |||
![Western Blot: BAF180/PB1 Antibody [NB100-79833] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Western-Blot-NB100-79833-img0012.jpg "Western Blot: BAF180/PB1 Antibody [NB100-79833] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NB100-79833] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PBRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunohistochemistry-Paraffin-NB100-79833-img0014.jpg)
![Western Blot: BAF180/PB1 Antibody [NB100-79833] - Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBRM1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Western-Blot-NB100-79833-img0011.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NB100-79833] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PBRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunohistochemistry-Paraffin-NB100-79833-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BAF180/PB1 Antibody [NB100-79833] - Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBRM1 antibody NB100-79833 used for IP at 3 ug per reaction. PBRM1 was also immunoprecipitated by rabbit anti-PBRM1 antibody NB100-79832. For blotting immunoprecipitated PBRM1, NB100-79833 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunoprecipitation-NB100-79833-img0010.jpg)
![Western Blot: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Whole cell lysate (50 ug) from Ba/F3 and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG.Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-BL-39-2C3-Western-Blot-NBP2-76400-img0016.jpg "Western Blot: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Whole cell lysate (50 ug) from Ba/F3 and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG.Chemiluminescence with an exposure time of 10 seconds.")
![Simple Western: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Detection of mouse PBRM1 by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (NBP2-76400) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally..](http://images.novusbio.com/fullsize/nbp2-76400_rabbit-monoclonal-baf180-pb1-antibody-bl-39-2c3-simple-western-3032023104450.jpg)
![Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-BL-39-2C3-Immunocytochemistry-Immunofluorescence-NBP2-76400-img0014.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-BL-39-2C3-Immunohistochemistry-Paraffin-NBP2-76400-img0011.jpg)
![Western Blot: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti- PBRM1 recombinant monoclonal antibody [BL-39-2C3] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-BL-39-2C3-Western-Blot-NBP2-76400-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-BL-39-2C3-Immunocytochemistry-Immunofluorescence-NBP2-76400-img0013.jpg)
![Immunoprecipitation: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Detection of human PBRM1 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal [BL-39-2C3] (NBP2-76400).](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-BL-39-2C3-Immunoprecipitation-NBP2-76400-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Detection of human PBRM1 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal [BL-3-2F2] (NBP2-76400) and rabbit anti-PBRM1 (NB100-1559 and NB100-1761).](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-BL-39-2C3-Immunoprecipitation-NBP2-76400-img0008.jpg)
![Flow Cytometry: BAF180/PB1 Antibody (BL-39-2C3) [NBP2-76400] - Antibody: Rabbit anti-PBRM1 recombinantmonoclonal [BL-39-2C3] or isotypecontrol (unshaded). Secondary: DyLight® 488-conjugatedgoat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76400_rabbit-monoclonal-baf180-pb1-antibody-bl-39-2c3-flow-cytometry-2910202491150..png)
![Western Blot: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Western blot analysis of extracts of various cell lines, using BAF180/PB1 Rabbit mAb (NBP3-15258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-9A8C4-Western-Blot-NBP3-15258-img0004.jpg "Western Blot: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Western blot analysis of extracts of various cell lines, using BAF180/PB1 Rabbit mAb (NBP3-15258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Immunohistochemistry of paraffin-embedded mouse kidney using BAF180/PB1 Rabbit mAb (NBP3-15258) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-9A8C4-Immunohistochemistry-Paraffin-NBP3-15258-img0003.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Immunohistochemistry of paraffin-embedded rat ovary using BAF180/PB1 Rabbit mAb (NBP3-15258) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-9A8C4-Immunohistochemistry-Paraffin-NBP3-15258-img0001.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Immunohistochemistry of paraffin-embedded human appendix using BAF180/PB1 Rabbit mAb (NBP3-15258) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-9A8C4-Immunohistochemistry-Paraffin-NBP3-15258-img0002.jpg)
![Immunohistochemistry: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Immunohistochemistry analysis of paraffin-embedded Human colon using BAF180/PB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15258_rabbit-baf180-pb1-mab-9a8c4-72202512443311.jpg)
![Immunohistochemistry: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using BAF180/PB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15258_rabbit-baf180-pb1-mab-9a8c4-72202512435353.jpg)
![Immunohistochemistry: BAF180/PB1 Antibody (9A8C4) [NBP3-15258] - Immunohistochemistry analysis of paraffin-embedded Human spleen using BAF180/PB1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15258_rabbit-baf180-pb1-mab-9a8c4-7220251245372.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining in human endometrium and skeletal muscle tissues. Corresponding PBRM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-CL0331-Immunohistochemistry-Paraffin-NBP2-30673-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining in human endometrium and skeletal muscle tissues. Corresponding PBRM1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-PBRM1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-CL0331-Western-Blot-NBP2-30673-img0013.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-CL0331-Immunohistochemistry-Paraffin-NBP2-30673-img0016.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human kidney cancer shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-CL0331-Immunohistochemistry-Paraffin-NBP2-30673-img0017.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human endometrium shows strong nuclear positivity in glandular and stromal cells](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-CL0331-Immunohistochemistry-Paraffin-NBP2-30673-img0018.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody (CL0331) [NBP2-30673] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-CL0331-Immunohistochemistry-Paraffin-NBP2-30673-img0020.jpg)
![Western Blot: BAF180/PB1 Antibody [NBP1-32025] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE PB1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Western-Blot-NBP1-32025-img0003.jpg "Western Blot: BAF180/PB1 Antibody [NBP1-32025] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE PB1 antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody [NBP1-32025] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32025-img0002.jpg)
![Immunohistochemistry-Paraffin: BAF180/PB1 Antibody [NBP1-32025] - Paraffin-embedded mouse duodenum. PB1 antibody [N1N2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunohistochemistry-Paraffin-NBP1-32025-img0004.jpg)
![Immunohistochemistry: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-BAF180/PB1 antibody (NBP3-32067) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32067) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32067_rabbit-baf180-pb1-mab-psh02-34-29820241416183.jpg "Immunohistochemistry: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-BAF180/PB1 antibody (NBP3-32067) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32067) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067] - Immunofluorescence analysis of paraffin-embedded human breast tissue labeling BAF180/PB1 with Rabbit anti-BAF180/PB1 antibody (NBP3-32067) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32067, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32067_rabbit-baf180-pb1-mab-psh02-34-298202414304111.jpg)
![Western Blot: BAF180/PB1 Antibody (PSH02-34) [NBP3-32067] - Western blot analysis of BAF180/PB1 on different lysates with Rabbit anti-BAF180/PB1 antibody (NBP3-32067) at 1/2,000 dilution.Lane 1: 293T cell lysateLane 2: HeLa cell lysateLane 3: SK-MEL-28 cell lysateLane 4: Jurkat cell lysateLane 5: NIH/3T3 cell lysateLane 6: C6 cell lysateLane 7: COS-1 cell lysateLysates/proteins at 15 µg/Lane.Predicted band size: 193 kDaObserved band size: 193 kDaExposure time: 1 minute 2 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32067_rabbit-baf180-pb1-mab-psh02-34-3920248485010.jpg)
![Immunoprecipitation: BAF180/PB1 Antibody [NB100-79832] - Detection of Human PB1/BAF180 by Western Blot of Immunoprecipitate. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PB1/BAF180 antibody NB100-79832 used for IP at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time 10 seconds.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunoprecipitation-NB100-79832-img0002.jpg "Immunoprecipitation: BAF180/PB1 Antibody [NB100-79832] - Detection of Human PB1/BAF180 by Western Blot of Immunoprecipitate. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PB1/BAF180 antibody NB100-79832 used for IP at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time 10 seconds.")
![BAF180/PB1 Antibody [NB100-79832] - Detection of human BAF180/PB1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PBRM1 antibody NB100-79832 (lot NB100-79832-3) used for IP at 6 ug per reaction. PBRM1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79832-2) and another rabbit anti-PBRM1 antibody. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-NB100-79832-img0004.jpg)
![Western Blot: BAF180/PB1 Antibody (SR1306) [NBP3-21933] - Western blot analysis using NBP3-21933 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21933_rabbit-baf180-pb1-mab-sr1306-57202315584842.jpg "Western Blot: BAF180/PB1 Antibody (SR1306) [NBP3-21933] - Western blot analysis using NBP3-21933 in HeLa cell lysate.")
![Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody [NBP2-55731] - Staining of human cell line RT4 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BAF180-PB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55731-img0001.jpg "Immunocytochemistry/Immunofluorescence: BAF180/PB1 Antibody [NBP2-55731] - Staining of human cell line RT4 shows localization to nucleoplasm. Antibody staining is shown in green.")
