| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-00441SS | |||
| Azide and BSA Free | NBP2-80838 | |||
MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A).View our protocol for Staining Membrane-associated Proteins." alt="Mouse bone marrow cells were stained with either (A) Rat Anti-Mouse Ly-6g (GR-1) Monoclonal Antibody (Catalog # MAB10371) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A).View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10371-SP | |||
| Carrier Free | FAB10371V-100UG | |||
| Carrier Free | FAB10371U-100UG | |||
| Carrier Free | FAB10371T-100UG | |||
| Carrier Free | FAB10371S-100UG | |||
| Carrier Free | FAB10371R-100UG | |||
| Carrier Free | FAB10371N-100UG | |||
| Carrier Free | FAB10371G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11978-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-53131SS | |||
| Azide and BSA Free | NBP2-80756 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-06691-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11784-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81053-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26160-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11979-0.025mg | |||
![Flow Cytometry: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - 1 ug/1^6 BALB/c bone marrow cells were stained with either rat IgG2b: FITC (as an isotype control) or rat anti-mouse Ly-6G: FITC. Large cells were then gated and analyzed on a flow cytometer.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Flow-Cytometry-NBP2-00441-img0002.jpg)
![Flow Cytometry: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - Analysis using the Allophycocyanin conjugate of NBP2-00441. Staining of C57BL/6 bone marrow cells with 0.125 ug of Rat IgG2b kappa Isotype Control APC (blue histogram) or 0.125 ug of Anti-Mouse Ly-6G (Gr-1) APC (purple histogram).](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Flow-Cytometry-NBP2-00441-img0008.jpg)
![Immunohistochemistry-Paraffin: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - Analysis of a FFPE tissue section of mouse bone marrow using 1:100 dilution of Lot E04586-1632 of Ly-6G antibody (clone RB6-8C5). The antibody generated specific staining in a subset of cells in the tested section.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Immunohistochemistry-Paraffin-NBP2-00441-img0007.jpg)
![Immunohistochemistry-Paraffin: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - Analysis of a FFPE tissue section of mouse bone marrow using 1:200 dilution of Lot A-1 of Ly-6G antibody (clone RB6-8C5). The antibody generated specific staining in a subset of cells in the tested section. The neutrophils (identifiable from typical nuclear morphology) showed stronger signal than the neighboring cells.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Immunohistochemistry-Paraffin-NBP2-00441-img0006.jpg)
![Flow Cytometry: Ly-6G/Ly-6C Antibody (RB6-8C5) [NBP2-00441] - Staining of mouse bone marrow with Anti-Mouse Ly-6G (Gr-1) FITC and Anti-Human/Mouse CD45R (B220) PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Flow-Cytometry-NBP2-00441-img0001.jpg)
![Immunohistochemistry: Ly-6G/Ly-6C Antibody (RB6-8C5) - BSA Free [NBP2-00441] - Silencing lnc-Lfar1 alleviates CCl4-induced proinflammatory activation of macrophages.Mice were treated with oil in combination with injection of lenti-NC (NC, n = 10), CCl4 in combination with injection of lenti-NC (NC + CCl4, n = 10), oil in combination with injection of lenti-lnc-Lfar1-shRNA (lnc-Lfar1-shRNA, n = 10), & CCl4 in combination with injection of lenti-lnc-Lfar1-shRNA (lnc-Lfar1-shRNA + CCl4, n = 10). a Immunohistochemistry analysis was performed to detect the expression of F4/80 & LY6C; scale bar = 400 μm for 10 × and 100 μm for 40×. b The protein level of F4/80, CD11b & LY6C was determined by western blot. GAPDH was used as an internal control. c The mRNA level of F4/80, Ly6c, Ccr2, Cd20, Il-6, iNos, Ccl5, Cxcl5, Cxcl9 & Cxcl10 was determined by qRT-PCR. *p < 0.05 vs NC, #p < 0.05 vs NC + CCl4. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32071306), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-00441_rat-monoclonal-ly-6g-ly-6c-antibody-rb6-8c5-31020241612214.jpg)
![Immunohistochemistry: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387] - Histopathology of lung sections collected at 24 h pbi from mice co-infected with mouse adapted influenza A/Puerto Rico/8/34 (H1N1) (PR8) and type 2 pneumococcal strain D39 variants. Serial lung sections were subjected to immunohistochemistry (IHC) for pneumococcus using Rabbit Anti-Streptococcus pneumoniae Polyclonal Antibody (Catalog # NB100-64502) at 4 ug/mL or neutrophils using Rat Anti-Ly-6G/Ly-6C Monoclonal Antibody (Catalog # NB600-1387) at 2 ug/mL. Representative images at 4x magnification with 60x magnification inset are shown. Images courtesy of Dr. Amanda P. Smith, UTHSC, TN //doi.org/10.1101/659557](http://images.novusbio.com/fullsize/Ly-6G6C-Antibody-NIMP-R14-Immunohistochemistry-NB600-1387-img0006.jpg "Immunohistochemistry: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387] - Histopathology of lung sections collected at 24 h pbi from mice co-infected with mouse adapted influenza A/Puerto Rico/8/34 (H1N1) (PR8) and type 2 pneumococcal strain D39 variants. Serial lung sections were subjected to immunohistochemistry (IHC) for pneumococcus using Rabbit Anti-Streptococcus pneumoniae Polyclonal Antibody (Catalog # NB100-64502) at 4 ug/mL or neutrophils using Rat Anti-Ly-6G/Ly-6C Monoclonal Antibody (Catalog # NB600-1387) at 2 ug/mL. Representative images at 4x magnification with 60x magnification inset are shown. Images courtesy of Dr. Amanda P. Smith, UTHSC, TN //doi.org/10.1101/659557")
![Immunohistochemistry-Frozen: Ly-6G/Ly-6C Antibody (NIMP-R14) [NB600-1387] - Staining for Ly-6G/Ly-6C FITC of frozen section of mouse spleen.](http://images.novusbio.com/fullsize/Ly-6G6C-Antibody-NIMP-R14-Immunohistochemistry-Frozen-NB600-1387-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Ly-6G/Ly-6C Antibody (RB6-8C5) - Chimeric [NBP3-11978] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of RB6-8C5 (NBP3-11978) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11978, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11978-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ly-6G/Ly-6C Antibody (RB6-8C5) - Chimeric [NBP3-11978] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of RB6-8C5 (NBP3-11978) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11978, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: Ly-6G/Ly-6C Antibody (RB6-8C5) - Chimeric [NBP3-11978] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of RB6-8C5 (NBP3-11978, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Ly-6G-Ly-6C-Antibody-RB6-8C5-Chimeric-Flow-Cytometry-NBP3-11978-img0002.jpg)
![Flow Cytometry: Gr-1/Ly-6G Antibody (1A8) [NBP2-53131] - Cells were pre-gated with live/dead and FSC-A/W to exclude dead cells and cell doublets. The single cell suspension was isolated from a spleen of an IL1-R KO (knockout) mouse and stained with the FITC-Conjugated CD45R antibody (1:100) for 30 minutes. Cells were analyzed by flow cytometer. This KO validation image was submitted via customer review. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Gr-1-Ly-6G-Antibody-1A8-Flow-Cytometry-NBP2-53131-img0002.jpg "<b>Genetic Strategies Validation. </b>Flow Cytometry: Gr-1/Ly-6G Antibody (1A8) [NBP2-53131] - Cells were pre-gated with live/dead and FSC-A/W to exclude dead cells and cell doublets. The single cell suspension was isolated from a spleen of an IL1-R KO (knockout) mouse and stained with the FITC-Conjugated CD45R antibody (1:100) for 30 minutes. Cells were analyzed by flow cytometer. This KO validation image was submitted via customer review. Image from the FITC version of this antibody.")
![<b>Genetic Strategies Validation. </b>Flow Cytometry: Gr-1/Ly-6G Antibody (1A8) [NBP2-53131] - Detection of Ly-6G in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse Ly-6G Monoclonal Antibody (Catalog # NBP2-53131, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).](http://images.novusbio.com/fullsize/Gr-1-Ly-6G-Antibody-1A8-Flow-Cytometry-NBP2-53131-img0001.jpg)
![Immunohistochemistry-Paraffin: Ly-6G Antibody (RM0064-6D17) [NBP2-11784] - Staining of paraffin sections of mouse kidney and spleen tissues from LPS exposed animals.](http://images.novusbio.com/fullsize/Ly-6G-Antibody-RM0064-6D17-Immunohistochemistry-Paraffin-NBP2-11784-img0001.jpg "Immunohistochemistry-Paraffin: Ly-6G Antibody (RM0064-6D17) [NBP2-11784] - Staining of paraffin sections of mouse kidney and spleen tissues from LPS exposed animals.")
![ELISA: Gr-1/Ly-6G Antibody (8I8) [NBP3-26160] - The Binding Activity of Gr-1/Ly-6G with Gr-1/Ly-6G Antibody.<br /> Activity: Measured by its binding ability in a functional ELISA. Immobilized cynomolgus monkey Gr-1/Ly-6G at 2 ug/mL can bind Gr-1/Ly-6G Antibody, the EC50 is 3.963-7.154 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26160_human-gr-1-ly-6g-mab-8i8-262202412272341.jpg "ELISA: Gr-1/Ly-6G Antibody (8I8) [NBP3-26160] - The Binding Activity of Gr-1/Ly-6G with Gr-1/Ly-6G Antibody.<br /> Activity: Measured by its binding ability in a functional ELISA. Immobilized cynomolgus monkey Gr-1/Ly-6G at 2 ug/mL can bind Gr-1/Ly-6G Antibody, the EC50 is 3.963-7.154 ng/mL.")
![ELISA: Gr-1/Ly-6G Antibody (8I8) [NBP3-26160] - The Binding Activity of Gr-1/Ly-6G with Gr-1/Ly-6G Antibody.<br />Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Gr-1/Ly-6G at 2 ug/mL can bind Gr-1/Ly-6G Antibody, the EC50 is 2.816-3.741 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26160_human-gr-1-ly-6g-mab-8i8-262202412272343.jpg)
![Flow Cytometry: Gr-1/Ly-6G Antibody (8I8) [NBP3-26160] - Untransfected HEK293T cells (green line) and transfected Human LY6G6D HEK293T stable cells (red line) were stained with anti-LY6G6D antibody (Gr-1/Ly-6G Antibody (8I8)) (2ug/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.](http://images.novusbio.com/fullsize/nbp3-26160_human-gr-1-ly-6g-mab-8i8-282202414183625.jpg)
![Western Blot: Ly-6G Overexpression Lysate (Adult Normal) [NBP2-06539] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ly-6G.](http://images.novusbio.com/fullsize/Ly-6G-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06539-img0001.jpg "Western Blot: Ly-6G Overexpression Lysate (Adult Normal) [NBP2-06539] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ly-6G.")
![Western Blot: Gr-1/Ly-6G Antibody [NBP3-09524] - Western blot analysis of Gr-1/Ly-6G in Fetal Lung lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/Gr-1-Ly-6G-Antibody-Western-Blot-NBP3-09524-img0001.jpg "Western Blot: Gr-1/Ly-6G Antibody [NBP3-09524] - Western blot analysis of Gr-1/Ly-6G in Fetal Lung lysates. Antibody dilution at 1.0ug/ml")
