CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="PITX2 was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Sheep Anti-Human PITX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7388) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7388-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55572-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57409-25ul | |||
![Immunocytochemistry/Immunofluorescence: PITX2 Antibody (2G6) [H00005308-M01] - Analysis of monoclonal antibody to PITX2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PITX2-Antibody-2G6-Immunocytochemistry-Immunofluorescence-H00005308-M01-img0003.jpg "Immunocytochemistry/Immunofluorescence: PITX2 Antibody (2G6) [H00005308-M01] - Analysis of monoclonal antibody to PITX2 on HeLa cell . Antibody concentration 10 ug/ml.")
![ELISA: PITX2 Antibody (2G6) [H00005308-M01] - Detection limit for recombinant GST tagged PITX2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PITX2-Antibody-2G6-ELISA-H00005308-M01-img0005.jpg)
![Western Blot:PITX2 Antibody (OTI1H10)[NBP3-45000]](http://images.novusbio.com/fullsize/nbp3-45000_mouse-pitx2-mab-oti1h10-western-blot-18120261611162.jpg "Western Blot:PITX2 Antibody (OTI1H10)[NBP3-45000]")
![Immunocytochemistry/Immunofluorescence: PITX2 Antibody [NBP2-55572] - Staining of human cell line U-2 OS shows localization to nucleus.](http://images.novusbio.com/fullsize/PITX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55572-img0001.jpg "Immunocytochemistry/Immunofluorescence: PITX2 Antibody [NBP2-55572] - Staining of human cell line U-2 OS shows localization to nucleus.")
![Western Blot: PITX2 Antibody [H00005308-D01P] - Analysis of PITX2 expression in human liver.](http://images.novusbio.com/fullsize/PITX2-Antibody-Western-Blot-H00005308-D01P-img0004.jpg "Western Blot: PITX2 Antibody [H00005308-D01P] - Analysis of PITX2 expression in human liver.")
![Western Blot: PITX2 Antibody [H00005308-D01P] - Analysis of PITX2 expression in transfected 293T cell line by PITX2 polyclonal antibody.Lane 1: PITX2 transfected lysate(30.30 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PITX2-Antibody-Western-Blot-H00005308-D01P-img0005.jpg)
![Proximity Ligation Assay: PITX2 Antibody [H00005308-D01P] - Analysis of protein-protein interactions between PITX2 and CTNNB1. HeLa cells were stained with anti-PITX2 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PITX2-Antibody-Proximity-Ligation-Assay-H00005308-D01P-img0003.jpg)
![Western Blot: PITX2 Antibody (3D2) [H00005308-M02] - PITX2 monoclonal antibody (M02), clone 3D2 Analysis of PITX2 expression in K-562.](http://images.novusbio.com/fullsize/PITX2-Antibody-3D2-Western-Blot-H00005308-M02-img0004.jpg "Western Blot: PITX2 Antibody (3D2) [H00005308-M02] - PITX2 monoclonal antibody (M02), clone 3D2 Analysis of PITX2 expression in K-562.")
![Western Blot: PITX2 Antibody (3D2) [H00005308-M02] - PITX2 monoclonal antibody (M02), clone 3D2 Analysis of PITX2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/PITX2-Antibody-3D2-Western-Blot-H00005308-M02-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PITX2 Antibody [NBP2-57409] - Staining of human cell line CACO-2 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PITX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57409-img0001.jpg "Immunocytochemistry/Immunofluorescence: PITX2 Antibody [NBP2-57409] - Staining of human cell line CACO-2 shows localization to nucleus. Antibody staining is shown in green.")
![Western Blot: PITX2 Overexpression Lysate (Adult Normal) [NBL1-14448] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PITX2.](http://images.novusbio.com/fullsize/PITX2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14448-img0002.jpg "Western Blot: PITX2 Overexpression Lysate (Adult Normal) [NBL1-14448] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PITX2.")
![Western Blot: PITX2 Overexpression Lysate (Adult Normal) [NBL1-14446] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PITX2.](http://images.novusbio.com/fullsize/PITX2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14446-img0002.jpg "Western Blot: PITX2 Overexpression Lysate (Adult Normal) [NBL1-14446] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PITX2.")
![Western Blot: PITX2 Antibody (6X1W3) [NBP3-33337] - Western blot analysis of lysates from HeLa cells, using PITX2(PITX2) Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-33337_rabbit-pitx2-mab-6x1w3-1012202411592882.jpg "Western Blot: PITX2 Antibody (6X1W3) [NBP3-33337] - Western blot analysis of lysates from HeLa cells, using PITX2(PITX2) Rabbit mAb at1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.")
![Western Blot: PITX2 Overexpression Lysate (Adult Normal) [NBL1-14447] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PITX2.](http://images.novusbio.com/fullsize/PITX2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14447-img0002.jpg "Western Blot: PITX2 Overexpression Lysate (Adult Normal) [NBL1-14447] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PITX2.")
