HAF017). A specific band was detected for p53R2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Human p53R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p53R2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3788-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76781-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90185-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-00079-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87368-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90186-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79410-5Plates | |||
![Western Blot: p53R2 Antibody [NBP1-76781] - Analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/p53R2-Antibody-Western-Blot-NBP1-76781-img0004.jpg "Western Blot: p53R2 Antibody [NBP1-76781] - Analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/Immunofluorescence: p53R2 Antibody [NBP1-76781] - Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/p53R2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76781-img0003.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody [NBP1-76781] - Human lung tissue with p53R2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/p53R2-Antibody-Immunohistochemistry-Paraffin-NBP1-76781-img0002.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - p53R2 antibody [HL1400] detects p53R2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. p53R2 stained by p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25588_rabbit-p53r2-mab-hl1400-azide-and-bsa-free-2120249393598.jpg "Immunohistochemistry-Paraffin: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - p53R2 antibody [HL1400] detects p53R2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. p53R2 stained by p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25588_rabbit-p53r2-mab-hl1400-azide-and-bsa-free-2120249393576.jpg)
![Western Blot: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25588_rabbit-p53r2-mab-hl1400-azide-and-bsa-free-212024939353.jpg)
![Western Blot: p53R2 Antibody (HL1400) - Azide and BSA Free [NBP3-25588] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with p53R2 antibody [HL1400] (NBP3-25588) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25588_rabbit-p53r2-mab-hl1400-azide-and-bsa-free-2120249432893.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human skeletal muscle with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0003.jpg "Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human skeletal muscle with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human tonsil with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0001.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody (001) [NBP2-90185] - Staining of human RRM2B in human squamous cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/p53R2-Antibody-001-Immunohistochemistry-Paraffin-NBP2-90185-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: p53R2 Antibody (001) [NBP2-90185] - Staining of RRM2B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90185 antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-90185_rabbit-monoclonal-p53r2-antibody-001-235202514492316.png)
![Western Blot: p53R2 Antibody [NBP2-19668] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/p53R2-Antibody-Western-Blot-NBP2-19668-img0002.jpg "Western Blot: p53R2 Antibody [NBP2-19668] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: p53R2 Antibody [NBP2-19668] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/p53R2-Antibody-Western-Blot-NBP2-19668-img0001.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody [NBP2-19668] - p53R2 antibody [N1C3] detects p53R2 protein at cytoplasm in mouse lung by immunohistochemical analysis. <br>Sample: Paraffin-embedded mouse lung. <br>p53R2 antibody [N1C3] (NBP2-19668) diluted at 1:500.<br><br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19668_rabbit-polyclonal-p53r2-antibody-910202420131211.jpg)
![Western Blot: p53R2 Antibody [NBP3-00079] - Anti-p53R2 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 50 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/p53R2-Antibody-Western-Blot-NBP3-00079-img0002.jpg "Western Blot: p53R2 Antibody [NBP3-00079] - Anti-p53R2 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 50 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: p53R2 Antibody [NBP3-00079] - Immunochemical staining of human RRM2B in human prostatic carcinomal with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/p53R2-Antibody-Immunohistochemistry-Paraffin-NBP3-00079-img0003.jpg)
![Immunohistochemistry-Paraffin: p53R2 Antibody [NBP3-00079] - Immunochemical staining of human p53R2 in human tonsil with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).](http://images.novusbio.com/fullsize/p53R2-Antibody-Immunohistochemistry-Paraffin-NBP3-00079-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: p53R2 Antibody [NBP1-87368] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/p53R2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87368-img0006.jpg "Immunocytochemistry/Immunofluorescence: p53R2 Antibody [NBP1-87368] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: p53R2 Antibody [NBP1-87368] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/p53R2-Antibody-Immunohistochemistry-Paraffin-NBP1-87368-img0005.jpg)
![Western Blot: p53R2 Overexpression Lysate (Adult Normal) [NBP2-11462] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for p53R2.](http://images.novusbio.com/fullsize/p53R2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11462-img0001.jpg "Western Blot: p53R2 Overexpression Lysate (Adult Normal) [NBP2-11462] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for p53R2.")
![Western Blot: p53R2 Antibody (074) [NBP2-90186] - Lane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:37 kDa](http://images.novusbio.com/fullsize/p53R2-Antibody-074-Western-Blot-NBP2-90186-img0001.jpg "Western Blot: p53R2 Antibody (074) [NBP2-90186] - Lane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:37 kDa")
![Sandwich ELISA: p53R2 Antibody Pair [HRP] [NBP2-79410] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/p53R2-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79410-img0001.jpg "Sandwich ELISA: p53R2 Antibody Pair [HRP] [NBP2-79410] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
