| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42191-0.025mg | |||
2034-NR) was serially diluted and captured by Mouse Anti-Human NrCAM Monoclonal Antibody (Catalog # MAB20341) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human NrCAM Isoform 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2034) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human NrCAM Fc Chimera (Catalog # 2034-NR) was serially diluted and captured by Mouse Anti-Human NrCAM Monoclonal Antibody (Catalog # MAB20341) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human NrCAM Isoform 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2034) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2034-SP | |||
HAF008). A specific band was detected for NrCAM at approximately 104-145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse/Rat NrCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8538) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for NrCAM at approximately 104-145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2034-SP | |||
2034-NR) was serially diluted and captured by Mouse Anti-Human NrCAM Monoclonal Antibody (Catalog # MAB20341) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human NrCAM Isoform 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2034) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human NrCAM Fc Chimera (Catalog # 2034-NR) was serially diluted and captured by Mouse Anti-Human NrCAM Monoclonal Antibody (Catalog # MAB20341) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human NrCAM Isoform 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2034) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB20341-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37379-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-11826-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88365-25ul | |||
![Western Blot: NrCAM Antibody (S364-51) [NBP2-42191] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51 (NBP2-42191). Load: 10 ug. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody (NBP2-42191) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa.](http://images.novusbio.com/fullsize/NrCAM-Antibody-S364-51-Western-Blot-NBP2-42191-img0003.jpg "Western Blot: NrCAM Antibody (S364-51) [NBP2-42191] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51 (NBP2-42191). Load: 10 ug. Primary Antibody: Mouse Anti-NrCam Monoclonal Antibody (NBP2-42191) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~160 kDa.")
![Immunocytochemistry/Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NrCAM Monoclonal Antibody, Clone S364-51 (NBP2-42191). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody (NBP2-42191) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) NrCAM Antibody (D) Composite.](http://images.novusbio.com/fullsize/NrCAM-Antibody-S364-51-Immunocytochemistry-Immunofluorescence-NBP2-42191-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/NrCAM-Antibody-S364-51-Immunocytochemistry-Immunofluorescence-NBP2-42191-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NrCAM Antibody (S364-51) [NBP2-42191] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-NrCAM Monoclonal Antibody, Clone S364-51 (NBP2-42191). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NrCAM Monoclonal Antibody (NBP2-42191) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) NrCAM Antibody. (D) Composite.](http://images.novusbio.com/fullsize/NrCAM-Antibody-S364-51-Immunocytochemistry-Immunofluorescence-NBP2-42191-img0005.jpg)
![Western Blot: NrCAM Antibody (7D8C5) [NBP2-37379] - Western blot analysis using NRCAM mAb against HEK293 (1) and NRCAM (AA: 1192-1255)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NrCAM-Antibody-7D8C5-Western-Blot-NBP2-37379-img0003.jpg "Western Blot: NrCAM Antibody (7D8C5) [NBP2-37379] - Western blot analysis using NRCAM mAb against HEK293 (1) and NRCAM (AA: 1192-1255)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NrCAM Antibody (7D8C5) [NBP2-37379] - Immunofluorescence analysis of HepG2 cells using NRCAM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NrCAM-Antibody-7D8C5-Immunocytochemistry-Immunofluorescence-NBP2-37379-img0004.jpg)
![Western Blot: NrCAM Antibody (7D8C5) [NBP2-37379] - Western blot analysis using NRCAM mAb against human NRCAM recombinant protein. (Expected MW is 32.7 kDa)](http://images.novusbio.com/fullsize/NrCAM-Antibody-7D8C5-Western-Blot-NBP2-37379-img0002.jpg)
![ELISA: NrCAM Antibody (7D8C5) [NBP2-37379] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NrCAM-Antibody-7D8C5-ELISA-NBP2-37379-img0001.jpg)
![Immunohistochemistry-Paraffin: NrCAM Antibody (MM0495-8E25) [NBP2-11826] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-11826. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.](http://images.novusbio.com/fullsize/NrCAM-Antibody-MM0495-8E25-Immunohistochemistry-Paraffin-NBP2-11826-img0001.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: NrCAM Antibody (MM0495-8E25) [NBP2-11826] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-11826. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.")
![Immunohistochemistry-Paraffin: NrCAM Antibody [NBP1-88365] - Staining of human pancreas shows membranous positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/NrCAM-Antibody-Immunohistochemistry-Paraffin-NBP1-88365-img0011.jpg "Immunohistochemistry-Paraffin: NrCAM Antibody [NBP1-88365] - Staining of human pancreas shows membranous positivity in exocrine glandular cells.")
![Immunohistochemistry-Paraffin: NrCAM Antibody [NBP1-88365] - Staining of human cerebellum shows positivity in neuropil.](http://images.novusbio.com/fullsize/NrCAM-Antibody-Immunohistochemistry-Paraffin-NBP1-88365-img0009.jpg)
![Immunohistochemistry-Paraffin: NrCAM Antibody [NBP1-88365] - Staining of human cerebral cortex shows positivity in neuropil.](http://images.novusbio.com/fullsize/NrCAM-Antibody-Immunohistochemistry-Paraffin-NBP1-88365-img0010.jpg)
![Western Blot: NrCAM Antibody [NBP1-59501] - HepG2 cell lysate, Antibody Titration: 2.5ug/ml](http://images.novusbio.com/fullsize/NrCAM-Antibody-Western-Blot-NBP1-59501-img0001.jpg "Western Blot: NrCAM Antibody [NBP1-59501] - HepG2 cell lysate, Antibody Titration: 2.5ug/ml")
![Immunohistochemistry-Paraffin: NrCAM Antibody [NBP1-59501] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/NrCAM-Antibody-Immunohistochemistry-Paraffin-NBP1-59501-img0003.jpg)
![Western Blot: NrCAM Antibody [NBP1-59490] - Jurkat cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/NrCAM-Antibody-Western-Blot-NBP1-59490-img0002.jpg "Western Blot: NrCAM Antibody [NBP1-59490] - Jurkat cell lysate, concentration 0.2-1 ug/ml.")
