| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-11197-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33157 | |||
| IHC-Prediluted | NBP2-44428 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44429 | |||
| Azide and BSA Free | NBP2-34757-0.1mg | |||
human intestinal organoid culture protocol. (A) The organoids were fixed and stained with a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 10x magnification. (B) The organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 20x magnification." alt="Adult stem cells isolated from human descending colon were cultured following the steps detailed in the human intestinal organoid culture protocol. (A) The organoids were fixed and stained with a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 10x magnification. (B) The organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (Catalog # MAB90981; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748; red) and DAPI (Catalog # 5748; blue). The image shown was taken at 20x magnification." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47685-0.1mg | |||
| IHC-Prediluted | NBP2-48391 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-23576SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21168-25ul | |||
| Azide and BSA Free | NBP3-44318 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48390 | |||
| Azide and BSA Free | NBP3-11491 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08239 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50521-0.025ml | |||
![Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody [996/1] NB120-11197AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/MUC2-Antibody-996-1-Flow-Cytometry-NB120-11197-img0006.jpg)
![Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on HeLa cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were fixed with 4% PFA and then permeabli](http://images.novusbio.com/fullsize/MUC2-Antibody-996-1-Flow-Cytometry-NB120-11197-img0003.jpg)
![Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on NTERA-2 cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were fixed with 4% PFA and then permea](http://images.novusbio.com/fullsize/MUC2-Antibody-996-1-Flow-Cytometry-NB120-11197-img0004.jpg)
![Flow (Intracellular): MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody (996/1) NB120-11197AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/MUC2-Antibody-996-1-Flow-Intracellular-NB120-11197-img0005.jpg)
![Immunohistochemistry-Paraffin: Mouse Monoclonal MUC2 Antibody (996/1) [NB120-11197] - Staining of mouse colon tissue using MUC2 Antibody. Colon, MUC2 -Green, DAPI-Blue. Antibody dilution - 1:100. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb120-11197_mouse-monoclonal-muc2-antibody-996-1-immunohistochemistry-paraffin-1652024102057..jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-paraffin normal human intestine (20X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/MUC2-Antibody-CCP58-Immunohistochemistry-Paraffin-NBP2-25221-img0003.jpg "Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-paraffin normal human intestine (20X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.")
![Western Blot: MUC2 Antibody (CCP58) [NBP2-25221] - MDA MB 231 cells and HCC827 human non-small cell lung cancer cell line lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/MUC2-Antibody-CCP58-Western-Blot-NBP2-25221-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC2 Antibody (CCP58).](http://images.novusbio.com/fullsize/MUC2-Antibody-CCP58-Immunohistochemistry-Paraffin-NBP2-25221-img0005.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-fixed, paraffin-embedded normal human intestine (10X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/MUC2-Antibody-CCP58-Immunohistochemistry-Paraffin-NBP2-25221-img0001.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (SPM296) [NBP2-32984] - Analysis of paraffin-embedded human colon tissue using MUC2 antibody. EDTA was used for antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize/MUC2-Antibody-SPM296-Immunohistochemistry-Paraffin-NBP2-32984-img0003.jpg "Immunohistochemistry-Paraffin: MUC2 Antibody (SPM296) [NBP2-32984] - Analysis of paraffin-embedded human colon tissue using MUC2 antibody. EDTA was used for antigen retrieval. Image from verified customer review.")
![Immunohistochemistry-Paraffin: MUC2 Antibody (SPM296) [NBP2-32984] - Formalin-fixed, paraffin-embedded human colon Carcinoma stained with MUC2 Monoclonal Antibody (SPM296).](http://images.novusbio.com/fullsize/MUC2-Antibody-SPM296-Immunohistochemistry-Paraffin-NBP2-32984-img0002.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (MLP/842) [NBP2-44431] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC2 Antibody (MLP/842).](http://images.novusbio.com/fullsize/MUC2-Antibody-MLP-842-Immunohistochemistry-Paraffin-NBP2-44431-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (MLP/842) [NBP2-44431] - Human Colon Carcinoma stained with MUC2 Monoclonal Antibody (MLP/842).](http://images.novusbio.com/fullsize/MUC2-Antibody-MLP-842-Immunohistochemistry-Paraffin-NBP2-44431-img0001.jpg)
![Western Blot: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of MUC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHSY-5Y cell lysateLane 2: SKBR3 cell lysate](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Western-Blot-NBP2-66961-img0011.jpg "Western Blot: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of MUC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHSY-5Y cell lysateLane 2: SKBR3 cell lysate")
![Immunocytochemistry/Immunofluorescence: MUC2 Antibody (JA10-92) [NBP2-66961] - Staining MUC2 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunocytochemistry-Immunofluorescence-NBP2-66961-img0010.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0014.jpg)
![Flow Cytometry: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of Hela cells with MUC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Flow-Cytometry-NBP2-66961-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MUC2 Antibody (JA10-92) [NBP2-66961] - Staining MUC2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunocytochemistry-Immunofluorescence-NBP2-66961-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MUC2 Antibody (JA10-92) [NBP2-66961] - Staining MUC2 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunocytochemistry-Immunofluorescence-NBP2-66961-img0003.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded human breast cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded human colon cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0005.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded human tonsil tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0006.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded rat brain tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0007.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded rat intestine tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0008.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-MUC2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0009.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Immunofluorescence staining of paraffin- embedded human colon using anti-MUC2 rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/100 dilution for 10 hours at 4C and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: MUC2 Antibody (JA10-92) [NBP2-66961] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MUC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MUC2-Antibody-JA10-92-Immunohistochemistry-Paraffin-NBP2-66961-img0013.jpg)
![Western Blot: MUC2 Antibody (4L5Y8) [NBP3-16481] - Western blot analysis of extracts of various cell lines, using MUC2 Rabbit mAb (NBP3-16481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MUC2-Antibody-4L5Y8-Western-Blot-NBP3-16481-img0004.jpg "Western Blot: MUC2 Antibody (4L5Y8) [NBP3-16481] - Western blot analysis of extracts of various cell lines, using MUC2 Rabbit mAb (NBP3-16481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: MUC2 Antibody (4L5Y8) [NBP3-16481] - Western blot analysis of extracts of Rat large intestine, using MUC2 Rabbit mAb (NBP3-16481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/MUC2-Antibody-4L5Y8-Western-Blot-NBP3-16481-img0005.jpg)
![Immunohistochemistry: MUC2 Antibody (4L5Y8) [NBP3-16481] - Immunohistochemistry analysis of paraffin-embedded Human colon using MUC2 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16481_rabbit-muc2-mab-4l5y8-52202511383779.jpg)
![Immunohistochemistry: MUC2 Antibody (4L5Y8) [NBP3-16481] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MUC2 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16481_rabbit-muc2-mab-4l5y8-5220251136510.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (SPM513) [NBP2-44430] - Formalin-fixed, paraffin-embedded human colon stained with MUC2 Mouse Monoclonal Antibody (SPM513).](http://images.novusbio.com/fullsize/MUC2-Antibody-SPM513-Immunohistochemistry-Paraffin-NBP2-44430-img0002.jpg "Immunohistochemistry-Paraffin: MUC2 Antibody (SPM513) [NBP2-44430] - Formalin-fixed, paraffin-embedded human colon stained with MUC2 Mouse Monoclonal Antibody (SPM513).")
![ELISA: Human MUC2 ELISA Kit (Colorimetric) [NBP2-76700] - Samples were spiked with high concentrations of Human MUC2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76700_human-muc2-elisa-kit-colorimetric-13220241685338.jpg "ELISA: Human MUC2 ELISA Kit (Colorimetric) [NBP2-76700] - Samples were spiked with high concentrations of Human MUC2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human MUC2 ELISA Kit (Colorimetric) [NBP2-76700] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76700_human-muc2-elisa-kit-colorimetric-432024917349.jpg)
![Immunohistochemistry-Paraffin: MUC2 Antibody (rMLP/842) [NBP3-07237] - Formalin-fixed, paraffin-embedded human stomach stained with MUC2 Recombinant Mouse Monoclonal Antibody (rMLP/842).](http://images.novusbio.com/fullsize/MUC2-Antibody-rMLP-842-Immunohistochemistry-Paraffin-NBP3-07237-img0001.jpg "Immunohistochemistry-Paraffin: MUC2 Antibody (rMLP/842) [NBP3-07237] - Formalin-fixed, paraffin-embedded human stomach stained with MUC2 Recombinant Mouse Monoclonal Antibody (rMLP/842).")
