VEGFR2/KDR/Flk-1: Novus Biologicals

AF644

VEGFR2/KDR/Flk-1 Antibody [Unconjugated]

(3 Reviews)

100 μg

AF644

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

VEGFR2/KDR/Flk-1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using 15 µg/mL Goat Anti-Mouse VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="VEGFR2/KDR/Flk-1 was detected in immersion fixed frozen sections of mouse embryo (14 d.p.c.) using 15 µg/mL Goat Anti-Mouse VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to mesenchymal cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

VEGFR2 was detected in acetone fixed cryosections of mouse kidney tissue using Goat Anti-Mouse VEGFR2/KDR/Flk-1 Polyclonal Antibody (Catalog # AF644) for 50 minutes at room temperature. Tissues were stained with rabbit anti-goat secondary antibody and HRP polymer-conjugated anti-rabbit IgG followed by AEC+Substrate Chromogen (red) followed by counterstaining with hematoxylin (blue). Experiments were carried out and the image was provided by Dr. Grietje Molema, University Medical Center Groningen, The Netherlands.

443-KD) inhibits Recombinant Mouse VEGF₁₆₄(Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse VEGF₁₆₄(5 ng/mL) activity elicited by Recombinant Mouse VEGFR2/KDR/Flk-1 Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_lightbox">

Recombinant Mouse VEGFR2/KDR/Flk-1 Fc Chimera (Catalog # <a class=

443-KD) inhibits Recombinant Mouse VEGF₁₆₄(Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse VEGF₁₆₄(5 ng/mL) activity elicited by Recombinant Mouse VEGFR2/KDR/Flk-1 Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF644). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_thumb" />

BM-PC express Notch pathway ligands/receptors and show increased expression of notch downstream targets during endothelial differentiation.A. Expression of Notch receptors and ligands in BM-PC was detected by RT-PCR. B. Expression of Notch downstream targets (Hes 1, Hey 1 and 2) was detected at different time points during BM-PC endothelial differentiation by quantitative real-time PCR. C. Representative images (×200) of BM-PC at day 20 of culture showing positivity for endothelial lineage specific markers, acetylated LDL, CD31 , Flk-1 and vWF with DAPI nuclear counterstaining in blue. D. Quantification of BM-PC positive cells for acetylated LDL, CD31 , Flk-1 and VWF after 20 days of culture. Each experiment was performed in triplicate and the mean presented (n = 3). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0003752), licensed under a CC-BY license. Not internally tested by R&D Systems.

Vascular alterations after intraocular VEGF-A injection. a Morphology of IB4-stained P6 wild-type retinal vessels at 4 h after administration of human VEGF-A165 (0.5 µl at a concentration of 5 μg μl−1). Note blunt appearance of the vessel front after VEGF-A injection but not for vehicle (PBS) control. Scale bar, 200 µm. b Quantitation of sprouts and filopodia at the front of the P6 vessel plexus after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test. c PDGFR beta + (green) pericytes are unaffected by short-term VEGF-A administration, whereas VEGFR2 immunosignals (white) are increased in IB4+ (red) ECs (arrowheads). Images shown correspond to insets in a. Scale bar, 100 µm. d Quantitation of VEGFR2 immunosignals intensity in the peripheral plexus of P6 retinas after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test. e Confocal images showing increased Esm1 immunostaining (white) in IB4+ (red) ECs in the peripheral plexus (arrowheads) after VEGF-A injection in P6 pups. Scale bar, 200 µm. f VEGF-A165 injection-mediated increase of Esm1 immunosignals (normalized to IB4+ EC area) in the peripheral capillary plexus but not at the edge of the angiogenic front in comparison to PBS-injected controls at P6. Error bars, s.e.m. p-values, Student’s t-test. NS, not statistically significant. g Short-term VEGF-A165 administration leads to clustering of Erg1+ (green) and IB4+ (red) ECs, as indicated, in thick sprout-like structures of P6 retinas. Panels in the center and on the right (scale bar, 20 µm) show higher magnification of the insets on the left (scale bar, 100 µm). Dashed lines in panels on the right outline IB4+ vessels. h Quantitation of EC density in the leading front vessel and emerging sprouts of the P6 angiogenic front after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test Image collected and cropped by CiteAb from the following publication (//pubme

Inactivation of Flt1 in PDGFR beta + cells. a Experimental scheme of tamoxifen administration for the generation of Flt1iPC mutants. b P6 control, Flt1iPC/+ and Flt1iPC retinas stained with isolectin B4 (IB4). Dashed circles indicate vessel-covered (yellow) and peripheral avascular (white) areas in the overview pictures (top). Scale bar, 500 µm. c Quantitation of body weight and radial outgrowth of the retinal vasculature in control, Flt1iPC/+ and Flt1iPC P6 pups. Error bars, s.e.m. p-values, one-way ANOVA. NS, not statistically significant. d Confocal images of the IB4-stained P6 control, Flt1iPC/+ and Flt1iPC retinal angiogenic front illustrating differences in sprout number and morphology. Scale bar, 100 µm. e Quantitation of sprouts and filopodia in P6 control, Flt1iPC/+ and Flt1iPC retinas. Error bars, s.e.m. p-values, one-way ANOVA and Tukey’s multiple comparison test. NS, not statistically significant. f Confocal images of IB4 (red), Erg1 (green) and VEGFR2 (white) stained P6 retinas highlighting the accumulation of EC nuclei and enhanced VEGFR2 immunosignals (arrowheads) in Flt1iPC sprouts. Vessels are outlined by dashed lines on the right panel. Scale bar, 100 µm. g Quantitation of EC proliferation (EdU+ Erg1+) at the angiogenic front, EC density in sprouts and leading front vessel and VEGFR2 immunosignals intensity in the angiogenic front of control and Flt1iPC P6 retinas. Error bars, s.e.m. p-values, Student’s t-test. h Esm1 (white) expression (arrowheads) in the angiogenic front (IB4+, red, first two columns) and detection of desmin+ pericytes (green, third column) in P6 control and Flt1iPC retinas. Scale bar, 100 µm. i Quantitation of Esm1+ proportion relative to vascular area (IB4+) in the angiogenic front of control and Flt1iPC P6 retinas. Error bars, s.e.m. p-values, Student’s t-test. j Confocal images of P6 retinas stained for NG2 (green) and IB4 (red) showing no significant changes in pericyte coverage in the front (first two columns) or the remodeling

AF644) at 1.7ug/mL with 1 hour incubation at room temperature followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and fibroblasts. " class="big_lightbox">

Formalin-fixed paraffin-embedded tissue sections of mouse kidney were probed for VEGFR2 mRNA (ACD RNAScope Probe, catalog #414818, Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-mouse VEGFR2 polyclonal antibody (R&D Systems catalog # <a class=

AF644) at 1.7ug/mL with 1 hour incubation at room temperature followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glomeruli and fibroblasts. " class="big_thumb" />

Mouse pancreas cryosections stained with VEGFR2 (yellow), labelling blood vessels. Image from a verified customer review.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF644U-100UG
Alexa Fluor 405	AF644V-100UG
Alexa Fluor 488	AF644G-100UG
Alexa Fluor 532	AF644X-100UG
Alexa Fluor 594	AF644T-100UG
Alexa Fluor 647	AF644R-100UG
Alexa Fluor 700	AF644N-100UG
Alexa Fluor 750	AF644S-100UG
Alexa Fluor Plus 405	AF644AFP405-100UG
Alexa Fluor Plus 488	AF644AFP488-100UG
Alexa Fluor Plus 555	AF644AFP555-100UG
Alexa Fluor Plus 594	AF644AFP594-100UG
Alexa Fluor Plus 647	AF644AFP647-100UG
Alexa Fluor Plus 680	AF644AFP680-100UG
Allophycocyanin	AF644A
Biotin	BAF644
CoraFluor 1	AF644CL1
DyLight 350	AF644D
DyLight 405	AF644E
DyLight 488	AF644K
DyLight 550	AF644L
DyLight 594	AF644M
DyLight 650	AF644W
DyLight 680	AF644Y
DyLight 755	AF644Z
FITC	AF644F
HRP	AF644H
Janelia Fluor 525	AF644JF525
Janelia Fluor 549	AF644I
Janelia Fluor 646	AF644J
Janelia Fluor 669	AF644JF669
PE	AF644P
PerCP	AF644C
mFluor Violet 450 SE	AF644MFV450
mFluor Violet 500 SE	AF644MFV500
mFluor Violet 610 SE	AF644MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF644-SP

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AF357

VEGFR2/KDR/Flk-1 Antibody [Unconjugated]

(4 Reviews)

(63 Publications)

100 μg

AF357

HAF017). Specific bands were detected for VEGFR2/KDR/Flk-1 at approximately 200-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). Specific bands were detected for VEGFR2/KDR/Flk-1 at approximately 200-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for VEGFR2/KDR/Flk-1 at approximately 200-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # <a class=

CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

357-KD) inhibits Recombinant Human VEGF₁₆₅(Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF₁₆₅(5 ng/mL) activity elicited by Recombinant Human VEGFR2/KDR/Flk-1 Fc Chi-mera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_lightbox">

Recombi-nant Human VEGFR2/KDR/Flk-1 Fc Chi-mera (Catalog # <a class=

357-KD) inhibits Recombinant Human VEGF₁₆₅(Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF₁₆₅(5 ng/mL) activity elicited by Recombinant Human VEGFR2/KDR/Flk-1 Fc Chi-mera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_thumb" />

<b>Biological Strategies Validation. </b>BMP7v exerts antiangiogenic effects and sensitizes chemoresistant CSCs to standard therapy. a Azan-Mallory staining on paraffin-embedded sections of xenografts derived from the injection of CRC sphere cells and treated for 4 weeks (6–9 weeks) with PBS (vehicle) or BMP7v. Data are representative of three independent experiments using different CRC sphere cell lines (CSC#2, 7, and 18). b Percentage of necrosis evaluated on paraffin-embedded sections of xenografts treated as in a. Data are shown as mean ± SD of three independent experiments. c Immunohistochemical analysis of CD31 and VEGFR2 (red staining) on paraffin-embedded sections of xenografts generated by the injection of CRC sphere cell lines and treated with PBS (vehicle), BMP4, or BMP7v. Green arrowheads indicate microvessels expressing CD31 or VEGFR2. Images are representative of three independent experiments using cells as in a. Nuclei were revealed by hematoxylin staining (blue). The scale bar represents 20 µm. d Number of microvessels positive for CD31 (left panel) and VEGFR2 (right panel) expression, evaluated on paraffin-embedded sections of xenografts treated as in c. Data are shown as mean ± SD of cells. MVD = microvessel density. e Fold change of viable cells in 35 CR-CSC lines treated with oxaliplatin/5-FU for 24 h. Dotted line indicates the threshold between chemoresistant (red) and sensitive CR-CSCs (green). f Cell viability percentage in chemoresistant CR-CSCs (R1-R4) pretreated with BMP7 for 3 days and with oxaliplatin/5-FU (oxa/5-FU) for additional 24 h as indicated. Data are shown as mean ± SD of three different experiments performed in the indicated R-CSCs. g Colony forming efficiency of CR-CSCs treated as in f and evaluated at 21 days. Representative soft-agar analyses are reported in the lower part of the graph. Bars show the mean ± SD of seven different CRC sphere cell lines (CSC#1–3, 5, 7, 10, and 18). h Tumor size of subcutaneous growth of the indicated CR-CSCs. Mice were treated for 4 we

Exogenous VEGF165 suppresses RASSF1A expression in normal epithelial and endothelial cells. Metastatic colon cancer cells (mCRC, (A) T84 and (B) Colo 205) were stimulated with VEGF165 as indicated before and cell metabolism was performed by MTT assay for 6 h (black dash), 24 h (green) and 48 h (pink). Immortalized benign protate ((C) RWPE1), human bronchial epithelial cells ((D) HBEpc), immortalized human breast epithelial cells ((E) MCF-10A), endothelial cells (F, EC) were stimulated with or without VEGF165 (12.5 and 25 ng/mL) for 24 h. Cell lysates were monitored by Western blot for p-VEGFR1, p-VEGFR2, RASSF1A, and GAPDH as indicated. Immunoblot was quantified by scanning densitometry and normalized against GAPDH expression for RWPE1 (G), HBEpc (H), MCF 10A (I) and EC (lower panel of (F)). Results are from three independent experiments and statistical significance was determined using one way-ANOVA followed Bonferroni test. (* p < 0.05, ** p < 0.01, *** p < 0.001). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30744076), licensed under a CC-BY license. Not internally tested by R&D Systems.

VEGF-C induces LEC tube formation.A, Prostatic LEC tube length at 4.5 hours post VEGF-C treatment was quantified using ImageJ. VEGF-C significantly increased the number of tubes formed compared to vehicle control. Data expressed as mean±s.e.m., n = 3, ***P<0.001 using One-way ANOVA, Bonferroni post-analysis. B, Western blotting analysis of VEGFR-2 and VEGFR-3 expression in lung, neonatal dermis and prostate LECs. beta -tubulin was used as a loading control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22745786), licensed under a CC-BY license. Not internally tested by R&D Systems.

357-KD) was serially diluted and captured by Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3573) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

357-KD) was serially diluted and captured by Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3573) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human VEGFR2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF357U-100UG
Alexa Fluor 405	AF357V-100UG
Alexa Fluor 488	AF357G-100UG
Alexa Fluor 532	AF357X-100UG
Alexa Fluor 594	AF357T-100UG
Alexa Fluor 647	AF357R-100UG
Alexa Fluor 700	AF357N-100UG
Alexa Fluor 750	AF357S-100UG
Alexa Fluor Plus 405	AF357AFP405-100UG
Alexa Fluor Plus 488	AF357AFP488-100UG
Alexa Fluor Plus 555	AF357AFP555-100UG
Alexa Fluor Plus 594	AF357AFP594-100UG
Alexa Fluor Plus 647	AF357AFP647-100UG
Alexa Fluor Plus 680	AF357AFP680-100UG
Allophycocyanin	AF357A
Biotin	BAF357
CoraFluor 1	AF357CL1
DyLight 350	AF357D
DyLight 405	AF357E
DyLight 488	AF357K
DyLight 550	AF357L
DyLight 594	AF357M
DyLight 650	AF357W
DyLight 680	AF357Y
DyLight 755	AF357Z
FITC	AF357F
HRP	AF357H
Janelia Fluor 525	AF357JF525
Janelia Fluor 549	AF357I
Janelia Fluor 646	AF357J
Janelia Fluor 669	AF357JF669
PE	AF357P
PerCP	AF357C
mFluor Violet 450 SE	AF357MFV450
mFluor Violet 500 SE	AF357MFV500
mFluor Violet 610 SE	AF357MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF357-SP

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357-KD

Recombinant Human VEGFR2/KDR Fc Chimera Protein

(41 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	357-KD-050/CF

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DVR200

Human VEGFR2/KDR Quantikine ELISA Kit

(38 Publications)

1 Kit

DVR200

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
HRP	SVR200
HRP	PDVR200

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MAB3572

VEGFR2/KDR/Flk-1 Antibody (89106) [Unconjugated]

(35 Publications)

100 μg

MAB3572

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox">

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." alt="HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3572, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

357-KD) inhibits Recombinant Human VEGF₁₆₅(Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF₁₆₅(10 ng/mL) activity elicited by Recombinant Human VEGFR2/KDR Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human VEGFR2/KDR Monoclonal Antibody (Catalog # MAB3572). The ND₅₀ is typically 10-50 ng/mL." class="big_lightbox">

Recombinant Human VEGFR2/KDR Fc Chimera (Catalog # <a class=

357-KD) inhibits Recombinant Human VEGF₁₆₅(Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF₁₆₅(10 ng/mL) activity elicited by Recombinant Human VEGFR2/KDR Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human VEGFR2/KDR Monoclonal Antibody (Catalog # MAB3572). The ND₅₀ is typically 10-50 ng/mL." class="big_thumb" />

Induction of endothelial markers in circulating CD14+ cells. CD14+ monocytes were isolated from adult PB, cultured in EGM-2 MV with or without MSCs, and then analyzed with flow cytometry. Dot plots from freshly isolated CD14+ cells (A) and after 4 days of co-culture with adipose-derived MSCs (green) (B) are shown. Flow-cytometry histograms in panels A and B show the expression of KDR and Tie2 in gated CD14+ cells. Isotype-matched controls are given. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24731246), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3572U-100UG
Alexa Fluor 405	FAB3572V-100UG
Alexa Fluor 488	FAB3572G-100UG
Alexa Fluor 532	FAB3572X
Alexa Fluor 594	FAB3572T-100UG
Alexa Fluor 647	FAB3572R-100UG
Alexa Fluor 700	FAB357N-025
Alexa Fluor 700	FAB357N-100
Alexa Fluor 750	FAB3572S-100UG
Alexa Fluor Plus 405	FAB3572AFP405-100UG
Alexa Fluor Plus 488	FAB3572AFP488-100UG
Alexa Fluor Plus 555	FAB3572AFP555-100UG
Alexa Fluor Plus 594	FAB3572AFP594-100UG
Alexa Fluor Plus 647	FAB3572AFP647-100UG
Alexa Fluor Plus 680	FAB3572AFP680-100UG
Allophycocyanin	FAB357A-025
Allophycocyanin	FAB357A-100
Allophycocyanin/Cy7	FAB3572APCCY7
Biotin	FAB3572B
CoraFluor 1	FAB3572CL1
DyLight 350	FAB3572D
DyLight 405	FAB3572E
DyLight 488	FAB3572K
DyLight 550	FAB3572L
DyLight 594	FAB3572M
DyLight 650	FAB3572W
DyLight 680	FAB3572Y
DyLight 755	FAB3572Z
Fluorescein	FAB357F-100
Fluorescein	FAB357F-025
HRP	FAB3572H
Janelia Fluor 525	FAB3572JF525
Janelia Fluor 549	FAB3572I
Janelia Fluor 646	FAB3572J
Janelia Fluor 669	FAB3572JF669
PE/Atto594	FAB3572PEATT594
PE/Cy5.5	FAB3572PECY55
PE/Cy7	FAB3572PECY7
Phycoerythrin	FAB357P-025
Phycoerythrin	FAB357P-100
mFluor Violet 450 SE	FAB3572MFV450
mFluor Violet 500 SE	FAB3572MFV500
mFluor Violet 610 SE	FAB3572MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3572-SP
Carrier Free	FAB3572R-100UG
Carrier Free	FAB3572S-100UG
Carrier Free	FAB3572T-100UG
Carrier Free	FAB3572U-100UG
Carrier Free	FAB3572V-100UG
Carrier Free	MAB3572-500
Carrier Free	FAB357P-025
Carrier Free	FAB357F-100
Carrier Free	FAB357P-100
Carrier Free	FAB357A-025
Carrier Free	FAB357A-100
Carrier Free	FAB357N-025
Carrier Free	FAB357N-100
Carrier Free	FAB357F-025
Carrier Free	FAB3572G-100UG

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MAB3571

VEGFR2/KDR/Flk-1 Antibody (89115) [Unconjugated]

(1 Review)

(23 Publications)

100 μg

MAB3571

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 25 µg/mL Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 25 µg/mL Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human kidney array using Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of HUVECs. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

VEGFR2/KDR/Flk-1 was detected in immersion fixed human umbilical vein endothelial cells (HUVECs; left panel) but is not detected in Hela human cervical epithelial carcinoma cell line (right panel) using Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of HUVECs. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3571U-100UG
Alexa Fluor 405	FAB3571V-100UG
Alexa Fluor 488	FAB3571G-100UG
Alexa Fluor 532	FAB3571X-100UG
Alexa Fluor 594	FAB3571T-100UG
Alexa Fluor 647	FAB3571R-100UG
Alexa Fluor 700	FAB3571N-100UG
Alexa Fluor 750	FAB3571S-100UG
Alexa Fluor Plus 405	FAB3571AFP405-100UG
Alexa Fluor Plus 488	FAB3571AFP488-100UG
Alexa Fluor Plus 555	FAB3571AFP555-100UG
Alexa Fluor Plus 594	FAB3571AFP594-100UG
Alexa Fluor Plus 647	FAB3571AFP647-100UG
Alexa Fluor Plus 680	FAB3571AFP680-100UG
Allophycocyanin	FAB3571A
Biotin	FAB3571B
CoraFluor 1	FAB3571CL1
DyLight 350	FAB3571D
DyLight 405	FAB3571E
DyLight 488	FAB3571K
DyLight 550	FAB3571L
DyLight 594	FAB3571M
DyLight 650	FAB3571W
DyLight 680	FAB3571Y
DyLight 755	FAB3571Z
FITC	FAB3571F
HRP	FAB3571H
Janelia Fluor 525	FAB3571JF525
Janelia Fluor 549	FAB3571I
Janelia Fluor 646	FAB3571J
Janelia Fluor 669	FAB3571JF669
PE	FAB3571P
PerCP	FAB3571C
mFluor Violet 450 SE	FAB3571MFV450
mFluor Violet 500 SE	FAB3571MFV500
mFluor Violet 610 SE	FAB3571MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3571-SP
Carrier Free	MAB3571-500

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NB100-530

VEGFR2/KDR/Flk-1 Antibody - BSA Free

(4 Publications)

0.1 ml

NB100-530

Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - V6.5 mouse embryonic stem cells (cat# NBP1-41162) stained with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).

Top: relative expression of CD36, GLUT2, and VEGFR2 in duodenum; n = 6–8. All values are expressed as model-based means, with error bars representing the confidence intervals (95% CI). *P value < 0.05. Bottom: Western blots of CD36 (75 kDa), GLUT2 (55 kDa), and VEGFR2 (105 kDa). Animals without a detectable band were excluded from the analysis. HIIT, high-intensity interval training; MICT, moderate-intensity continuous training; CON, control group.

Western Blot: VEGFR2/KDR/Flk-1 Antibody [NB100-530] - Top: relative expression of CD36, GLUT2, & VEGFR2 in duodenum; n = 6–8. All values are expressed as model-based means, with error bars representing the confidence intervals (95% CI). *P value < 0.05. Bottom: Western blots of CD36 (75 kDa), GLUT2 (55 kDa), & VEGFR2 (105 kDa). Animals without a detectable band were excluded from the analysis. HIIT, high-intensity interval training; MICT, moderate-intensity continuous training; CON, control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28183816), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemical analyses of VEGFR2, Ang-2, vWF and CD34 in the peri-infarct area of the ischemic brain. a Representative merged images counterstained with DAPI (nuclear staining) (blue). Ang-2, VEGFR2, vWF and CD34 antibodies are represented by the green color (arrows). b Relative fluorescence intensity showed that Ang-2, VEGFR2, and CD34 were increased in the exercise groups. After exercise, vWF was decreased. No significant differences were observed between groups (p > 0.05). Sham: (n = 4); MCAO: (n = 4); MCAO-30m: (n = 4); MCAO-60m: (n = 4). Bar = 50 um. Error bars represent S.E.M Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30941660), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-530AF350
Alexa Fluor 405	NB100-530AF405
Alexa Fluor 488	NB100-530AF488
Alexa Fluor 532	NB100-530AF532
Alexa Fluor 594	NB100-530AF594
Alexa Fluor 647	NB100-530AF647
Alexa Fluor 700	NB100-530AF700
Alexa Fluor 750	NB100-530AF750
Alexa Fluor Plus 405	NB100-530AFP405
Alexa Fluor Plus 488	NB100-530AFP488
Alexa Fluor Plus 555	NB100-530AFP555
Alexa Fluor Plus 594	NB100-530AFP594
Alexa Fluor Plus 647	NB100-530AFP647
Alexa Fluor Plus 680	NB100-530AFP680
Allophycocyanin	NB100-530APC
Biotin	NB100-530B
CoraFluor 1	NB100-530CL1
DyLight 350	NB100-530UV
DyLight 405	NB100-530V
DyLight 488	NB100-530G
DyLight 550	NB100-530R
DyLight 594	NB100-530DL594
DyLight 650	NB100-530C
DyLight 680	NB100-530FR
DyLight 755	NB100-530IR
FITC	NB100-530F
HRP	NB100-530H
Janelia Fluor 525	NB100-530JF525
Janelia Fluor 549	NB100-530JF549
Janelia Fluor 646	NB100-530JF646
Janelia Fluor 669	NB100-530JF669
PE	NB100-530PE
PerCP	NB100-530PCP
mFluor Violet 450 SE	NB100-530MFV450
mFluor Violet 500 SE	NB100-530MFV500
mFluor Violet 610 SE	NB100-530MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-530SS

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AF1766

VEGFR2/KDR/Flk-1 [p Tyr1214] Antibody [Unconjugated]

(14 Publications)

50 μg

AF1766

HAF008). A specific band was detected for VEGFR2/KDR/Flk-1 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western Blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1766) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). A specific band was detected for VEGFR2/KDR/Flk-1 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1766) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for VEGFR2/KDR/Flk-1 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>VEGFR2/KDR/Flk-1 phosphorylated at Y1214 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human Phospho-VEGFR2/KDR/Flk-1 (Y1214) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1766) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; <a class=

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

FoxM1 and RASSF1A expression is inversely co-related in colon cancer tissues. (A) Tissue extranction from both normal and colon cancer patient for monitoring the translational level of FoxM1 and RASSF1A by immunoblot. Quantification of RASSF1A (B) and FoxM1 (C) by scanning densitometry. GAPDH was used as a loading control. (D) T84 and Colo 205 cells were treated with or without AGP IC50 (45 µM) for 48 h. Cell lysates were analyzed by Western blot for FoxM1 and GAPDH expression. (E) Quantitative estimations of FoxM1 levels determined by densitometry measurements of western blots from three independent experiments after normalization with GAPDH (p < 0.001). T84 and Colo 205 cells were treated with or without AGP as indicated before and the transcriptional level were determined by qRT-PCR for (F) FoxM1 and (G) PTTG1. Bar graphs show quantitative results normalized to GAPDH mRNA levels. Results are from three independent experiments and statistical significance was determined using one way-ANOVA followed Bonferroni test. (* p < 0.05, ** p < 0.01, *** p < 0.001). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30744076), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1766-SP

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NBP2-36429

VEGFR2/KDR/Flk-1 Antibody (2C6) - BSA Free

(2 Publications)

0.1 mg

NBP2-36429

Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.

Flow (Cell Surface): VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - HepG2 cells were stained (surface) with antibody (orange) or isotype control, mouse IgG1 (blue). Positive staining observed using FITC conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis

Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of human normal kidney with antibody at 5 ug/ml concentration. The cuboidal epithelial cells of of various tubules/ducts showed a specific cytoplasmic immunopositivity of VEGF R2/KDR/Flk-1 protein.

Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of Human SCC of Esophagus with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm while the tumor stroma was negative for VEGF R2/KDR/Flk-1 immunopositivity.

Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of Human Hepatocellular Carcinoma with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.

Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Aanalysis of FFPE transverse section of normal human skeletal muscle with antibody at 5 ug/ml concentration. The myocytes showed a moderate diffused to granular staining in the cytoplasm.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-36429AF350
Alexa Fluor 405	NBP2-36429AF405
Alexa Fluor 488	NBP2-36429AF488
Alexa Fluor 532	NBP2-36429AF532
Alexa Fluor 594	NBP2-36429AF594
Alexa Fluor 647	NBP2-36429AF647
Alexa Fluor 700	NBP2-36429AF700
Alexa Fluor 750	NBP2-36429AF750
Alexa Fluor Plus 405	NBP2-36429AFP405
Alexa Fluor Plus 488	NBP2-36429AFP488
Alexa Fluor Plus 555	NBP2-36429AFP555
Alexa Fluor Plus 594	NBP2-36429AFP594
Alexa Fluor Plus 647	NBP2-36429AFP647
Alexa Fluor Plus 680	NBP2-36429AFP680
Allophycocyanin	NBP2-36429APC
Biotin	NBP2-36429B
CoraFluor 1	NBP2-36429CL1
DyLight 350	NBP2-36429UV
DyLight 405	NBP2-36429V
DyLight 488	NBP2-36429G
DyLight 550	NBP2-36429R
DyLight 594	NBP2-36429DL594
DyLight 650	NBP2-36429C
DyLight 680	NBP2-36429FR
DyLight 755	NBP2-36429IR
FITC	NBP2-36429F
HRP	NBP2-36429H
Janelia Fluor 525	NBP2-36429JF525
Janelia Fluor 549	NBP2-36429JF549
Janelia Fluor 646	NBP2-36429JF646
Janelia Fluor 669	NBP2-36429JF669
PE	NBP2-36429PE
PerCP	NBP2-36429PCP
mFluor Violet 450 SE	NBP2-36429MFV450
mFluor Violet 500 SE	NBP2-36429MFV500
mFluor Violet 610 SE	NBP2-36429MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-36429-0.025mg
Azide and BSA Free	NBP2-81009

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NBP2-36428

VEGFR2/KDR/Flk-1 Antibody (1B6) - BSA Free

(1 Review)

0.1 mg

NBP2-36428

Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of FFPE transverse section of normal human skeletal muscle with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The myocytes showed a moderate diffused to granular staining in the cytoplasm.

Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - HepG2 cells were stained (surface) with VEGF R2/KDR/Flk-1 antibody (clone: 2B5.1B6, orange) or isotype control (mouse IgG1, blue) and positive staining observed using FITC conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis

Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Detection in HUVEC cells at 1:50

Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of FFPE tissue section of human normal kidney with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The cuboidal epithelial cells of of various tubules/ducts showed a specific cytoplasmic immunopositivity of protein.

Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of FFPE tissue section of human liver cancer with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The cancer cells showed a specific and strong staining in the cytoplasm.

Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (1B6) [NBP2-36428] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with mouse monoclonal VEGF R2/KDR/Flk-1 antibody (clone 1B6) at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-36428AF350
Alexa Fluor 405	NBP2-36428AF405
Alexa Fluor 488	NBP2-36428AF488
Alexa Fluor 532	NBP2-36428AF532
Alexa Fluor 594	NBP2-36428AF594
Alexa Fluor 647	NBP2-36428AF647
Alexa Fluor 700	NBP2-36428AF700
Alexa Fluor 750	NBP2-36428AF750
Alexa Fluor Plus 405	NBP2-36428AFP405
Alexa Fluor Plus 488	NBP2-36428AFP488
Alexa Fluor Plus 555	NBP2-36428AFP555
Alexa Fluor Plus 594	NBP2-36428AFP594
Alexa Fluor Plus 647	NBP2-36428AFP647
Alexa Fluor Plus 680	NBP2-36428AFP680
Allophycocyanin	NBP2-36428APC
Allophycocyanin/Cy7	NBP2-36428APCCY7
Biotin	NBP2-36428B
CoraFluor 1	NBP2-36428CL1
DyLight 350	NBP2-36428UV
DyLight 405	NBP2-36428V
DyLight 488	NBP2-36428G
DyLight 550	NBP2-36428R
DyLight 594	NBP2-36428DL594
DyLight 650	NBP2-36428C
DyLight 680	NBP2-36428FR
DyLight 755	NBP2-36428IR
FITC	NBP2-36428F
HRP	NBP2-36428H
Janelia Fluor 525	NBP2-36428JF525
Janelia Fluor 549	NBP2-36428JF549
Janelia Fluor 646	NBP2-36428JF646
Janelia Fluor 669	NBP2-36428JF669
PE	NBP2-36428PE
PE/Atto594	NBP2-36428PEATT594
PE/Cy5.5	NBP2-36428PECY55
PE/Cy7	NBP2-36428PECY7
PerCP	NBP2-36428PCP
mFluor Violet 450 SE	NBP2-36428MFV450
mFluor Violet 500 SE	NBP2-36428MFV500
mFluor Violet 610 SE	NBP2-36428MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-36428SS
Azide and BSA Free	NBP2-81008

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MVR200B

Mouse VEGFR2/Flk-1 Quantikine ELISA Kit

(14 Publications)

1 Kit

MVR200B

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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DYC1766-2

Human Phospho-VEGFR2/KDR DuoSet IC ELISA, 2 Plate

(13 Publications)

1 Kit (for 2 Plates)

DYC1766-2

293-VE) for five minutes to induce tyrosine phosphorylation of VEGF R2. Dilutions of HUVEC lysates were analyzed by this ELISA (Catalog # DYC1766) and IP-Western blot (inset). IPs were performed using an anti-VEGF R2 monoclonal antibody and anti-mouse IgG agarose. Immunoblots were incubated with a biotinylated anti-phospho-tyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-VEGF R2 (p-VEGF R2). Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection. Blots were stripped and total VEGF R2 (VEGF R2) was detected using a biotinylated polyclonal anti-VEGF R2 antibody (Catalog # BAF357). " class="big_lightbox">

<b>Biological Strategies Validation. </b>Human umbilical vein endothelial cells (HUVEC) were treated with 100 ng/mL recombinant human VEGF165 (Catalog # <a class=

293-VE) for five minutes to induce tyrosine phosphorylation of VEGF R2. Dilutions of HUVEC lysates were analyzed by this ELISA (Catalog # DYC1766) and IP-Western blot (inset). IPs were performed using an anti-VEGF R2 monoclonal antibody and anti-mouse IgG agarose. Immunoblots were incubated with a biotinylated anti-phospho-tyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-VEGF R2 (p-VEGF R2). Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection. Blots were stripped and total VEGF R2 (VEGF R2) was detected using a biotinylated polyclonal anti-VEGF R2 antibody (Catalog # BAF357). " alt="Human umbilical vein endothelial cells (HUVEC) were treated with 100 ng/mL recombinant human VEGF165 (Catalog # 293-VE) for five minutes to induce tyrosine phosphorylation of VEGF R2. Dilutions of HUVEC lysates were analyzed by this ELISA (Catalog # DYC1766) and IP-Western blot (inset). IPs were performed using an anti-VEGF R2 monoclonal antibody and anti-mouse IgG agarose. Immunoblots were incubated with a biotinylated anti-phospho-tyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-VEGF R2 (p-VEGF R2). Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection. Blots were stripped and total VEGF R2 (VEGF R2) was detected using a biotinylated polyclonal anti-VEGF R2 antibody (Catalog # BAF357). " class="big_thumb" />

<b>Biological Strategies Validation. </b>HUVECs were untreated or treated with 100 ng/mL recombinant human VEGF165 for five minutes. ELISA and IP-Western blot (inset) analyses were performed using 100 μg and 1000 μg of lysate, respectively. IP-Western blots were performed as described in Figure 1.

357-KD), VEGF R1 (Catalog # 321-FL) or VEGF R3 (Catalog # 349-F4) were added to 100 μg lysate and analyzed by this ELISA. Competition was observed only with recombinant VEGF R2. " class="big_lightbox">

<b>Biological Strategies Validation. </b>HUVECs were treated with 100 ng/mL of human recombinant VEGF165 for five minutes. The indicated amounts of extracellular domains of recombinant VEGF R2 (Catalog # <a class=

357-KD), VEGF R1 (Catalog # 321-FL) or VEGF R3 (Catalog # 349-F4) were added to 100 μg lysate and analyzed by this ELISA. Competition was observed only with recombinant VEGF R2. " class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Biotin	DYC1766E
Biotin	DYC1766-5

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Products Related to Gene-ID: 3791

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Recombinant Human VEGFR2/KDR Fc Chimera Protein

Human VEGFR2/KDR Quantikine ELISA Kit

VEGFR2/KDR/Flk-1 Antibody (89106) [Unconjugated]

VEGFR2/KDR/Flk-1 Antibody (89115) [Unconjugated]

VEGFR2/KDR/Flk-1 Antibody - BSA Free

VEGFR2/KDR/Flk-1 [p Tyr1214] Antibody [Unconjugated]

VEGFR2/KDR/Flk-1 Antibody (2C6) - BSA Free

VEGFR2/KDR/Flk-1 Antibody (1B6) - BSA Free

Mouse VEGFR2/Flk-1 Quantikine ELISA Kit

Human Phospho-VEGFR2/KDR DuoSet IC ELISA, 2 Plate