| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87679-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43897 | |||
| BSA Free | NBP2-52872-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14104 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26501-50ul | |||
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0027.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Aquaporin-4 Antibody [NBP1-87679] - Analysis in human brain tissue.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP1-87679-img0017.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody [NBP1-87679] - Staining of mouse cerebellum shows positivity in astrocytes, mainly in the molecular layer.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0029.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human cerebellum shows moderate to strong membranous positivity in astrocytes, mainly in the molecular layer.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0030.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0031.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human liver shows no positivity in hepatocytes, as expected.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0032.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human stomach shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87679-img0033.jpg)
![Immunohistochemistry-Frozen: Aquaporin-4 Antibody [NBP1-87679] - Mouse trigeminal ganglion stained with Aquaporin-4 antibody at 1:5000. Image submitted by a verified customer review](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Frozen-NBP1-87679-img0036.jpg)
![Induction of atrogin 1 mRNA and protein expression by rotator cuff tear (RCT). (a) Expression of transcripts encoding the E3 ubiquitin-protein ligases atrogin 1, MuRF1, and UBR2 in injured muscle after RCT (n = 12 per group). (b) Atrogin 1 protein expression in muscle isolated at different stages after RCT (n = 7 per group). Densitometric analyses of the western blots are shown on the right. Data represent means +/- standard errors of the means. *P < 0.05. (c) Immunofluorescence (IF) microscopy analyses of muscle isolated at different stages after RCT. The red signal represents atrogin 1 protein. The negative control (secondary antibody only) for the IF showed no specific signal (data not shown). (d) Loss of AQP4 protein by overexpression of atrogin 1. C2C12 cells were treated with an adenovirus expressing atrogin 1 [Ad-atrogin 1; multiplicity of infection (MOI) = 50, 100, 200] for 48 h, and western blot analysis was performed with each designated antibody. (e) Monomeric ubiquitin protein expression in muscle cells isolated at different stages after RCT. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32843684), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87679_rabbit-polyclonal-aquaporin-4-antibody-western-blot-13220261319313.jpg)
![Increased levels of HMGB1 protein in muscle injured by rotator cuff tear (RCT). (a) Expression level of HMGB1 protein in muscle isolated at different stages after RCT (n = 7 per group). Densitometric analyses of the western blots are shown on the right. Data represent means +/- standard errors of the means. *P < 0.05. (b) Immunofluorescence (IF) microscopy analyses of muscle cells isolated at different stages after RCT. The green signal represents HMGB1 protein. The negative control (secondary antibody only) for the IF showed no specific signal (data not shown). (c) Expression of atrogin 1 mRNA in C2C12 myotubes treated with 200 ng/mL recombinant mouse HMGB1 for the indicated time periods. (d) Atrogin 1, ubiquitin, and AQP4 protein levels in C2C12 myotubes treated with 200 ng/mL HMGB1 and an adenovirus expressing atrogin 1 [Ad-atrogin 1; multiplicity of infection (MOI) = 100] for 48 h. (e) Ubiquitination of AQP4 caused by HMGB1 and atrogin 1. C2C12 myotubes were treated with 200 ng/mL HMGB1 and Ad-atrogin 1 (MOI = 100) with or without MG132. After 48 h of incubation, cell lysates were prepared for a ubiquitination assay using anti-AQP4 and anti-ubiquitin antibodies. (f) Expression of atrogin 1 mRNA in C2C12 myotubes exposed to 5 μM SB203580 (a p38 MAPK inhibitor), LY294002 (a PI3K inhibitor), BAY 11-7082 (an NF-kappa B inhibitor), PD98059 (an MEK inhibitor), or SP600125 (a JNK inhibitor). Cells were exposed to each inhibitor for 1 h prior to treatment with 200 ng/mL HMGB1 for 24 h. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32843684), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87679_rabbit-polyclonal-aquaporin-4-antibody-western-blot-132202613153820.jpg)
![Western Blot: Aquaporin-4 Antibody [NBP3-12902] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Aquaporin-4 antibody (NBP3-12902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP3-12902-img0004.jpg "Western Blot: Aquaporin-4 Antibody [NBP3-12902] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Aquaporin-4 antibody (NBP3-12902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein at astrocyte cells by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aquaporin-4 protein stained by Aquaporin-4 antibody (NBP3-12902) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (NBP2-43561) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12902-img0002.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Aquaporin-4 stained by Aquaporin-4 antibody (NBP3-12902) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP3-12902-img0005.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Aquaporin-4 antibody (NBP3-12902) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP3-12902-img0001.jpg)
![Immunohistochemistry-Frozen: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Aquaporin-4 protein stained by Aquaporin-4 antibody (NBP3-12902) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Frozen-NBP3-12902-img0003.jpg)
![Western Blot: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Western blot analysis of various lysates, using Aquaporin-4Rabbit mAb at 1:900 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-912202414154378.jpg "Western Blot: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Western blot analysis of various lysates, using Aquaporin-4Rabbit mAb at 1:900 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-31120251744416.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-31120251704787.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-3112025173144.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Human lung cancer tissue using Aquaporin-4 (AQP4) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-immunohistochemistry-paraffin-1352025122932..png)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining in human cerebral cortex and liver tissues. Corresponding AQP4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Western-Blot-NBP2-52872-img0013.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0008.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human cerebellum shows strong positivity in neuropil, mainly in the molecular layer.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0009.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human stomach shows moderate to strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0010.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-CL0178-Immunohistochemistry-Paraffin-NBP2-52872-img0011.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Formalin-fixed, paraffin-embedded human small intestine stained with Aquaporin-4 antibody (AQP4/3324). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-AQP4-3324-Immunohistochemistry-Paraffin-NBP3-13952-img0003.jpg "Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Formalin-fixed, paraffin-embedded human small intestine stained with Aquaporin-4 antibody (AQP4/3324). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Formalin-fixed, paraffin-embedded human brain stained with Aquaporin-4 antibody (AQP4/3324). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-AQP4-3324-Immunohistochemistry-Paraffin-NBP3-13952-img0001.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Formalin-fixed, paraffin-embedded human small intestine stained with Aquaporin-4 antibody (AQP4/3324). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-AQP4-3324-Immunohistochemistry-Paraffin-NBP3-13952-img0002.jpg)
![Protein Array: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Aquaporin-4 Antibody (AQP4/3324).](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-AQP4-3324-Protein-Array-NBP3-13952-img0005.jpg)
![Western Blot: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Western blot analysis of Aquaporin-4 on different lysates with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 35 kDa Observed band size: 28/52 kDa Exposure time: Lane 1: 31 seconds; Lane 2-3: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32046) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32046_rabbit-aquaporin-4-mab-je59-76-278202416331236.jpg "Western Blot: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Western blot analysis of Aquaporin-4 on different lysates with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 35 kDa Observed band size: 28/52 kDa Exposure time: Lane 1: 31 seconds; Lane 2-3: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32046) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Aquaporin-4 Antibody (JE59-76) [NBP3-32046] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Aquaporin-4 antibody (NBP3-32046) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32046) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32046_rabbit-aquaporin-4-mab-je59-76-29820241492659.jpg)
![Western Blot: Aquaporin-4 Antibody [NBP2-87018] - WB Suggested Anti-AQP4 Antibody. Titration: 1.0 ug/ml. Positive Control: MDA-MB-435S Whole CellAQP4 is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB435 cells](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP2-87018-img0001.jpg "Western Blot: Aquaporin-4 Antibody [NBP2-87018] - WB Suggested Anti-AQP4 Antibody. Titration: 1.0 ug/ml. Positive Control: MDA-MB-435S Whole CellAQP4 is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB435 cells")
![Western Blot: Aquaporin-4 Antibody [NBP2-87018] - Host: Mouse. Target Name: AQP4. Sample Tissue: Mouse Heart. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Western-Blot-NBP2-87018-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free [NBP3-43560] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with NBP3-43560 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom: NBP3-43560, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43560_rabbit-aquaporin-4-mab-d15107-chimeric-azide-bsa-free-74202522141214.png "Immunocytochemistry /Immunofluorescence: Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free [NBP3-43560] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with NBP3-43560 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom: NBP3-43560, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.")
![Western Blot: Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free [NBP3-43560] - Unboiled (A) and boiled (B) mouse skeletal muscle tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with NBP3-43560 at 1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-43560_rabbit-aquaporin-4-mab-d15107-chimeric-azide-bsa-free-74202522113012.png)
![Immunohistochemistry: Aquaporin-4 Antibody (22F10) [NBP3-26501] - Image of Aquaporin-4 Antibody (22F10) diluted at 1:50 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.33% DAB.](http://images.novusbio.com/fullsize/nbp3-26501_rabbit-aquaporin-4-mab-22f10-2722024845326.jpg "Immunohistochemistry: Aquaporin-4 Antibody (22F10) [NBP3-26501] - Image of Aquaporin-4 Antibody (22F10) diluted at 1:50 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.33% DAB.")