| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF-421-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76337-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 405 | NBP1-42772AFP405 | |||
| Alexa Fluor Plus 488 | NBP1-42772AFP488 | |||
| Alexa Fluor Plus 555 | NBP1-42772AFP555 | |||
| Alexa Fluor Plus 594 | NBP1-42772AFP594 | |||
| Alexa Fluor Plus 647 | NBP1-42772AFP647 | |||
| Alexa Fluor Plus 680 | NBP1-42772AFP680 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| PE | NBP2-27252 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25229 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 5194-IL-025/CF | |||
![Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Analysis of 1 ng of recombinant human IL-17 with IL-17 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-76337-img0013.jpg "Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Analysis of 1 ng of recombinant human IL-17 with IL-17 antibody at 1 ug/ml.")
![Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Loading: 10 ug of lysates per lane. Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 min Lane 1: 1 ug/mL Lane 2: 2 ug/mL](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-76337-img0012.jpg)
![Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Colon Tissue. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IL-17/IL-17A antibody at 2 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-2711202420414565.gif)
![Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Heart Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart issue labeling IL-17/IL-17A with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-271120241928814.jpg)
![Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Human Tonsil Tissue.Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-17/IL-17A antibody at 5 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-2711202420312943.gif)
![Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse Thymus Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17/IL-17A with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-2711202419293859.gif)
![Immunohistochemistry: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Human Lymph Node.Immunohistochemical analysis of paraffin-embedded human lymph node tissue using anti-IL-17/IL-17A antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-2711202420171759.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody - BSA Free [NBP1-76337] - Validation of IL-17/IL-17A in Mouse A-20 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse A-20 Cells labeling IL-17/IL-17A with at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76337_rabbit-polyclonal-il-17-il-17a-antibody-271120242042535.gif)
![N/A IL-17/IL-17A [HRP]](http://images.novusbio.com/fullsize2/IL17_HS170_ELISA_1173.jpg "N/A IL-17/IL-17A [HRP]")
![N/A IL-17/IL-17A [HRP]](http://images.novusbio.com/fullsize2/IL17_HS170_ELISA_1174.jpg)
![N/A IL-17A/F Heterodimer [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL17A_F_Heterodimer_DY5390_ELISA_2326.jpg "N/A IL-17A/F Heterodimer [Biotin]")
![N/A IL-17A/F Heterodimer [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL17A_F_Heterodimer_DY5194_ELISA_2086.jpg "N/A IL-17A/F Heterodimer [Biotin]")
![Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-42772-img0011.jpg "Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.")
![Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the Biotin conjugate of NBP1-42772. Detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-42772-img0006.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the HRP conjugate of NBP1-42772. Detection of Lane 1: Recombinant Rat IL-17A. Load: 50 ng per lane. Used at a dilution of 1:1,000 o/n at 4C.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-42772-img0007.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of mouse recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP1-42772-img0010.jpg)
![N/A IL-17/IL-17A [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL17_DY5848_ELISA_2138.jpg "N/A IL-17/IL-17A [Biotin]")
![N/A IL-17A/F Heterodimer [HRP]](http://images.novusbio.com/fullsize2/DATA_IL17A_F_Heterodimer_M17AF0_ELISA_925.jpg "N/A IL-17A/F Heterodimer [HRP]")
![N/A IL-17A/F Heterodimer [HRP]](http://images.novusbio.com/fullsize2/IL-17A_F_Heterodimer_M17AF0_ELISA_228.jpg)
![Western Blot: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis of IL-17A in 1) human, 2) mouse and 3) rat full-length recombinant IL-17A protein using NBP2-27338 at 1 ug/ml.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-4H1524-Western-Blot-NBP2-27338-img0004.jpg "Western Blot: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis of IL-17A in 1) human, 2) mouse and 3) rat full-length recombinant IL-17A protein using NBP2-27338 at 1 ug/ml.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis using the PE conjugate of NBP2-25229. IL-17A positive cells in PMA/IM stimulated fresh PBMC: PBMC stimulated for 6h with PMA (25ng/ml) & IM (250ng/ml), in the presence of BFA. Cells were surface stained with CD4 APC, fixed & permeabilised for intracellular staining with PE conjugated](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-4H1524-Flow-Cytometry-NBP2-27338-img0007.jpg)
![Block/Neutralize: IL-17/IL-17A Antibody (4H1524) [NBP2-27338] - Analysis using Azide Free version of NBP2-27338. Neutralization by Human IL-17A Antibody. Secretion of IL-6 by 3T3 cells stimulated with recombinant human IL-17A (20ng/ml) is neutralized by increasing concentrations of IL-17A monoclonal antibody. Antibody](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-4H1524-Block-Neutralize-NBP2-27338-img0006.jpg)
![Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] - IL-17/IL-17A Antibody (TC11-18H10) [NBP1-72027] - Treatment with HBOT or PFD decreased IL-17A expression in the AD lesions. IHC for IL-17A on mouse skin. Scale bar is 100 um. The number of IL-17A-expressing cells was counted in five high-power fields (HPF) chosen at random in each slide by three different pathologists. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0109297) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-TC11-18H10-Immunohistochemistry-Paraffin-NBP1-72027-img0003.jpg "Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] - IL-17/IL-17A Antibody (TC11-18H10) [NBP1-72027] - Treatment with HBOT or PFD decreased IL-17A expression in the AD lesions. IHC for IL-17A on mouse skin. Scale bar is 100 um. The number of IL-17A-expressing cells was counted in five high-power fields (HPF) chosen at random in each slide by three different pathologists. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0109297) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody (TC11-18H10) [NBP1-72027] - Analysis of anti-IL-17 antibody of mouse spleen at concentration 10 ug/ml.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-TC11-18H10-Immunohistochemistry-Paraffin-NBP1-72027-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-17/IL-17A Antibody (TC11-18H10) - Azide and BSA Free [NBP1-72027] - TG2-dependent chemokine production in keratinocytes is critical for IL-17-producing CCR6+ gamma δT-cell & neutrophil dermal infiltration.a, b Effect of DMSO- or IMQ-treated WT- or TG2−/−-CM on CCR6+ gamma δT-cell (a) & CD11b+ Ly-6G+ cell (b) migration according to Transwell migration assays. Flow cytometric analysis of the percentage of cells migrating toward the CM in the presence or absence of anti-CXCL1 (a) & anti-CCL20 (b) antibodies (n = 3/group). c, d WT & TG2−/− mouse-derived CCR6+ gamma δT-cell (c) & CD11b+ Ly-6G+ cell (d) migration in response to recombinant mouse CCL20 & CXCL1, respectively. Data represent the mean ± SEM (n = 3/group). *p < 0.05; **p < 0.01. e, f Cells were isolated from the back skin of WT & TG2−/− mice treated with IMQ for four consecutive days & analyzed by flow cytometry. Representative flow cytometric profiles of CCR6+ gamma δTCRlow cells (e) & CD11bhigh Ly-6G+ cells (f). The percentage of cells is shown. Data represent the mean ± SEM (n = 9/group). g Skin sections were immunostained with CCR6 (green)- & IL-17 (red)-specific antibodies. Nuclei were stained with DAPI (blue). Scale bar, 100 μm. h–j Back skin was separated into epidermis & dermis & analyzed by RT-PCR for IL-17-producing gamma δT-cell markers (h) (Ccr6 & Il23r), IL17 & related cytokines (i) (Il17a, Il17f, & Il22), & myeloid cell-derived cytokines (j) (Il12a & Il23a). Data represent the mean ± SEM (n = 11/group). *p < 0.05; **p < 0.01 vs. WT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32355189), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-72027_rat-il-17-il-17a-mab-tc11-18h10-azide-and-bsa-free-310202416171471.jpg)