FAB554P) and either (A) Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Catalog # MAB7211) or (B) Rat IgG
2BIsotype Control (Catalog #
MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog #
F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005)." class="big_lightbox">
FAB554P) and either (A) Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Catalog # MAB7211) or (B) Rat IgG
2BIsotype Control (Catalog #
MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog #
F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005)." alt="Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog #
FAB554P) and either (A) Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Catalog # MAB7211) or (B) Rat IgG
2BIsotype Control (Catalog #
MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog #
F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005)." class="big_thumb" />
FAB554P) and either (A) Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Catalog # MAB7211) or (B) Rat IgG2BIsotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)." alt="Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Catalog # MAB7211) or (B) Rat IgG2BIsotype Control (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)." class="big_thumb" />
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Immunohistochemistry-Paraffin-NBP3-13294-img0003.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0001.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0004.jpg)
![Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon cancer. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Immunohistochemistry-Paraffin-NBP3-13294-img0002.jpg)
![ELISA: IL-17/IL-17A ELISA Kit [HRP] [NBP1-92705] - Standard curve](http://images.novusbio.com/fullsize/IL-17-IL-17A-ELISA-Kit-[HRP]-ELISA-NBP1-92705-img0001.jpg "ELISA: IL-17/IL-17A ELISA Kit [HRP] [NBP1-92705] - Standard curve")
![Western Blot: IL-17/IL-17A Antibody (408H6.01/mAb25) [DDX0336P-100] - 1-Human IL17 is a N -glycosylated dimer.2-Human IL17 digested by endoglycosidase -F.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-408H6-01-mAb25-Western-Blot-DDX0336P-100-img0001.jpg "Western Blot: IL-17/IL-17A Antibody (408H6.01/mAb25) [DDX0336P-100] - 1-Human IL17 is a N -glycosylated dimer.2-Human IL17 digested by endoglycosidase -F.")
![Flow Cytometry: IL-17/IL-17A Antibody (408H6.01/mAb25) [DDX0336P-100]](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-408H6-01-mAb25-Flow-Cytometry-DDX0336P-100-img0002.jpg)
![N/A IL-17/IL-17A [HRP]](http://images.novusbio.com/fullsize/elisa/mhs170_mouse-il-17-quantikine-hs-elisa-kit-25320211288.jpg "N/A IL-17/IL-17A [HRP]")
![N/A IL-17/IL-17A [HRP]](http://images.novusbio.com/fullsize/elisa/mhs170_mouse-il-17-quantikine-hs-elisa-kit-2532021121352.jpg)
![N/A IL-17/IL-17A [HRP]](http://images.novusbio.com/fullsize/elisa/qk317_human-il-17-quantikine-quickit-elisa-kit-209202193534.jpg "N/A IL-17/IL-17A [HRP]")
![N/A IL-17/IL-17A [HRP]](http://images.novusbio.com/fullsize/elisa/qk317_human-il-17-quantikine-quickit-elisa-kit-209202193632.jpg)
