| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56352SS | |||
MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with human 5-HT7 and eGFP was stained with (A) Mouse Anti-Human 5-HT7 Monoclonal Antibody (Catalog # MAB10325) or (B) isotype control antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22298-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-68957-25ul | |||
![Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.](http://images.novusbio.com/fullsize/5-HT7-Antibody-Western-Blot-NB100-56352-img0003.jpg "Western Blot: 5-HT7 Antibody [NB100-56352] - Analysis of 5-HT7R in A) human brain, B) mouse brain, C) rat brain, and D) human SK-N-SH neuroblastoma cell lysate using this antibody.")
![Simple Western: 5-HT7 Antibody [NB100-56352] - Time-dependent effects of subacute administration of effective dose of escitalopram (5 mg/kg/day) for 3 & 7 days on expression of 5-HT7R in the thalamic plasma membrane fraction (Panel (A)). Ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT7R in the thalamic plasma membrane fraction. Panel (B) indicates the pseudo-gel images using capillary immunoblotting. ** p < 0.01 vs. control by one-way analysis of variance with Tukey’s post-hoc test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33572981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56352_rabbit-polyclonal-5-ht7-antibody-310202415384177.jpg)
![Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598] - Sections of rat brain. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 Aum.](http://images.novusbio.com/fullsize/5-HT7-Antibody-Immunohistochemistry-Paraffin-NBP1-46598-img0002.jpg "Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598] - Sections of rat brain. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 Aum.")
![Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP1-46598] - Sections of rat brain. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm.](http://images.novusbio.com/fullsize/5-HT7-Antibody-Immunohistochemistry-Paraffin-NBP1-46598-img0001.jpg)
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # ![Western Blot: 5-HT7 Antibody (4E6D2) [NBP3-15870] - Analysis of extracts of various cell lines, using 5-HT7 Rabbit mAb (NBP3-15870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/5-HT7-Antibody-4E6D2-Western-Blot-NBP3-15870-img0003.jpg "Western Blot: 5-HT7 Antibody (4E6D2) [NBP3-15870] - Analysis of extracts of various cell lines, using 5-HT7 Rabbit mAb (NBP3-15870) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: 5-HT7 Antibody (4E6D2) [5-HT7] - Confocal imaging of paraffin-embedded Rat brain using 5-HT7 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) .DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15870_rabbit-5-ht7-mab-4e6d2-5220251726041.jpg)
![Immunocytochemistry/ Immunofluorescence: 5-HT7 Antibody (4E6D2) [5-HT7] - Confocal imaging of paraffin-embedded Mouse brain using 5-HT7 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) .DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15870_rabbit-5-ht7-mab-4e6d2-52202517284954.jpg)
![Western Blot: 5-HT7 Antibody [NBP1-44051] - (1ug/ml) Analysis of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/5-HT7-Antibody-Western-Blot-NBP1-44051-img0003.jpg "Western Blot: 5-HT7 Antibody [NBP1-44051] - (1ug/ml) Analysis of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP3-14527] - Immunohistochemistry of formalin-fixed, paraffin-embedded human cerebellum after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/5-HT7-Antibody-Immunohistochemistry-Paraffin-NBP3-14527-img0001.jpg "Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP3-14527] - Immunohistochemistry of formalin-fixed, paraffin-embedded human cerebellum after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: 5-HT7 Antibody [NBP3-14527] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, glioblastoma, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/5-HT7 Antibody-Immunohistochemistry-Paraffin-NBP3-14527-img0002.jpg)
![Western Blot: 5-HT7 Antibody (SR1954) [NBP3-22298] - Western blot analysis using NBP3-22298 in U-87MG cell lysate.](http://images.novusbio.com/fullsize/nbp3-22298_rabbit-5-ht7-mab-sr1954-57202315545147.jpg "Western Blot: 5-HT7 Antibody (SR1954) [NBP3-22298] - Western blot analysis using NBP3-22298 in U-87MG cell lysate.")
![Immunohistochemistry: 5-HT7 Antibody [NBP2-68957] - Immunohistochemical staining of human hypothalamus shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/5-HT7-Antibody-Immunohistochemistry-NBP2-68957-img0001.jpg "Immunohistochemistry: 5-HT7 Antibody [NBP2-68957] - Immunohistochemical staining of human hypothalamus shows strong cytoplasmic positivity in neuronal cells.")
![Western Blot: 5HT7 Receptor Overexpression Lysate (Native) [NBP2-06701] - 5HT7 Receptor Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 5-HTR7.](http://images.novusbio.com/fullsize/5HT7-Receptor-Overexpression-Lysate-Native-Western-Blot-NBP2-06701-img0002.jpg "Western Blot: 5HT7 Receptor Overexpression Lysate (Native) [NBP2-06701] - 5HT7 Receptor Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 5-HTR7.")
