| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77000-0.025mg | |||
HAF007). A specific band for APAF-1 was detected at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 2 x 105cells (lane 1) and 7 x 104cells (lane 2). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human APAF-1 Monoclonal Antibody (Catalog # MAB868) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for APAF-1 was detected at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB868-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61868-0.025ml | |||
![Western Blot: APAF-1 Antibody [NBP1-77000] - Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/APAF-1-Antibody-Western-Blot-NBP1-77000-img0006.jpg "Western Blot: APAF-1 Antibody [NBP1-77000] - Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP1-77000] - Staining of Heart tissue at 1 ug/ml.](http://images.novusbio.com/fullsize/APAF-1-Antibody-Immunohistochemistry-Paraffin-NBP1-77000-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: APAF-1 Antibody - BSA Free [NBP1-77000] - Immunofluorescence of APAF-1 in K562 cells with APAF-1 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77000_rabbit-polyclonal-apaf-1-antibody-2711202419251515.jpg)
![Western Blot: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis using APAF1 mAb against human APAF1 (AA: 1138-1237) recombinant protein. (Expected MW is 37 kDa)](http://images.novusbio.com/fullsize/APAF-1-Antibody-2H9A1-Western-Blot-NBP2-61868-img0004.jpg "Western Blot: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis using APAF1 mAb against human APAF1 (AA: 1138-1237) recombinant protein. (Expected MW is 37 kDa)")
![Immunocytochemistry/Immunofluorescence: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis of Hela cells using APAF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/APAF-1-Antibody-2H9A1-Immunocytochemistry-Immunofluorescence-NBP2-61868-img0003.jpg)
![Flow Cytometry: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis of Hela cells using APAF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/APAF-1-Antibody-2H9A1-Flow-Cytometry-NBP2-61868-img0002.jpg)
![Western Blot: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis using APAF1 mAb against HEK293 (1) and APAF1 (AA: 1138-1237)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/APAF-1-Antibody-2H9A1-Western-Blot-NBP2-61868-img0005.jpg)
![ELISA: APAF-1 Antibody (2H9A1) [NBP2-61868] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/APAF-1-Antibody-2H9A1-ELISA-NBP2-61868-img0001.jpg)
![Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP2-15420] - Paraffin-embedded mouse duodenum. APAF1 antibody [C3], C-term diluted at 1:400.](http://images.novusbio.com/fullsize/APAF-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15420-img0004.jpg "Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP2-15420] - Paraffin-embedded mouse duodenum. APAF1 antibody [C3], C-term diluted at 1:400.")
![Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP2-15420] - Paraffin-embedded breast ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/APAF-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15420-img0002.jpg)
![Immunohistochemistry-Paraffin: APAF-1 Antibody [NBP2-15420] - Paraffin-embedded rat liver. APAF1 antibody [C3], C-term diluted at 1:400.](http://images.novusbio.com/fullsize/APAF-1-Antibody-Immunohistochemistry-Paraffin-NBP2-15420-img0003.jpg)
![Western Blot: APAF-1 Antibody (2E12) [NBP2-80092] - Analysis using anti-Apaf-1 (human), MAb (2E12) on several human cell lines and one monkey cell line (COS).](http://images.novusbio.com/fullsize/APAF-1-Antibody-2E12-Western-Blot-NBP2-80092-img0001.jpg "Western Blot: APAF-1 Antibody (2E12) [NBP2-80092] - Analysis using anti-Apaf-1 (human), MAb (2E12) on several human cell lines and one monkey cell line (COS).")
![Immunohistochemistry-Paraffin: APAF-1 Antibody (2E12) [NBP2-80092] - Immunohistochemical staining of Apaf-1 using anti-Apaf-1 (human), mAb (2E12) in formalin-fixed and paraffin-embedded (FFPE) human kidney tissue (10 ug/mL).](http://images.novusbio.com/fullsize/APAF-1-Antibody-2E12-Immunohistochemistry-Paraffin-NBP2-80092-img0002.jpg)
![ELISA: Human APAF-1 ELISA Kit (Colorimetric) [NBP2-68232] - Samples were spiked with high concentrations of Human APAF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68232_human-apaf-1-elisa-kit-colorimetric-132202416211374.jpg "ELISA: Human APAF-1 ELISA Kit (Colorimetric) [NBP2-68232] - Samples were spiked with high concentrations of Human APAF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human APAF-1 ELISA Kit (Colorimetric) [NBP2-68232] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68232_human-apaf-1-elisa-kit-colorimetric-432024857422.jpg)
![Western Blot: APAF-1 Antibody (13F11) - BSA Free [NBP2-80093] - Analysis using Apaf-1 (mouse/rat), MAb (13F11) (NBP2-80093) on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1 KO mice.](http://images.novusbio.com/fullsize/APAF-1-Antibody-13F11-BSA-Free-Western-Blot-NBP2-80093-img0001.jpg "Western Blot: APAF-1 Antibody (13F11) - BSA Free [NBP2-80093] - Analysis using Apaf-1 (mouse/rat), MAb (13F11) (NBP2-80093) on purified cells from mouse tissues. The ~130kDa band corresponds to endogenous Apaf-1, which is absent in cells from Apaf-1 KO mice.")
![Immunoprecipitation: APAF-1 Antibody (13F11) - BSA Free [NBP2-80093] - On mouse thymocytes using APAF-1 (mouse/rat), MAb (13F11) (NBP2-80093).](http://images.novusbio.com/fullsize/APAF-1-Antibody-13F11-BSA-Free-Immunoprecipitation-NBP2-80093-img0002.jpg)
![Western Blot: APAF-1 Antibody (18H2) - BSA Free [NBP2-80094] - Analysis using APAF-1, mAb (18H2) (NBP2-80094) on lysates from mouse cell lines.](http://images.novusbio.com/fullsize/APAF-1-Antibody-18H2-BSA-Free-Western-Blot-NBP2-80094-img0001.jpg "Western Blot: APAF-1 Antibody (18H2) - BSA Free [NBP2-80094] - Analysis using APAF-1, mAb (18H2) (NBP2-80094) on lysates from mouse cell lines.")
![Immunoprecipitation: APAF-1 Antibody (18H2) - BSA Free [NBP2-80094] - On mouse thymocytes using APAF-1, mAb (18H2) (NBP2-80094).](http://images.novusbio.com/fullsize/APAF-1-Antibody-18H2-BSA-Free-Immunoprecipitation-NBP2-80094-img0002.jpg)
![SDS-Page: Recombinant Human APAF-1 Protein [H00000317-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-APAF-1-Protein-SDS-Page-H00000317-Q01-img0001.jpg "SDS-Page: Recombinant Human APAF-1 Protein [H00000317-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Mouse APAF-1 ELISA Kit (Colorimetric) [NBP2-68233] - Samples were spiked with high concentrations of Mouse APAF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68233_mouse-apaf-1-elisa-kit-colorimetric-132202416215341.jpg "ELISA: Mouse APAF-1 ELISA Kit (Colorimetric) [NBP2-68233] - Samples were spiked with high concentrations of Mouse APAF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse APAF-1 ELISA Kit (Colorimetric) [NBP2-68233] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68233_mouse-apaf-1-elisa-kit-colorimetric-4320249507.jpg)
![ELISA: Human APAF-1 ELISA Kit (Chemiluminescence) [NBP2-68231] - Samples were spiked with high concentrations of Human APAF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68231_human-apaf-1-elisa-kit-chemiluminescence-132202416204099.jpg "ELISA: Human APAF-1 ELISA Kit (Chemiluminescence) [NBP2-68231] - Samples were spiked with high concentrations of Human APAF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human APAF-1 ELISA Kit (Chemiluminescence) [NBP2-68231] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68231_human-apaf-1-elisa-kit-chemiluminescence-4320248582549.jpg)
![Western Blot: APAF1 Peptide [NBP1-77000PEP] - Apaf1 peptide is used for blocking the activity of Anti-Apaf1. Incubating the peptide with equal volume of antibody for 30 min at 37C usually completely blocks the antibody activity in Western blotting.](http://images.novusbio.com/fullsize/APAF1-Peptide-Western-Blot-NBP1-77000PEP-img0001.jpg "Western Blot: APAF1 Peptide [NBP1-77000PEP] - Apaf1 peptide is used for blocking the activity of Anti-Apaf1. Incubating the peptide with equal volume of antibody for 30 min at 37C usually completely blocks the antibody activity in Western blotting.")
